Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   BLL42_RS12075 Genome accession   NZ_CP017886
Coordinates   2494986..2497061 (-) Length   691 a.a.
NCBI ID   WP_071552301.1    Uniprot ID   A0A1J0EKN8
Organism   Pseudomonas frederiksbergensis strain ERDD5:01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2489986..2502061
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BLL42_RS12060 (BLL42_11795) - 2491608..2493203 (-) 1596 WP_071552298.1 AAA family ATPase -
  BLL42_RS12065 (BLL42_11800) aroB 2493214..2494314 (-) 1101 WP_071552299.1 3-dehydroquinate synthase -
  BLL42_RS12070 (BLL42_11805) aroK 2494463..2494981 (-) 519 WP_071552300.1 shikimate kinase AroK -
  BLL42_RS12075 (BLL42_11810) pilQ 2494986..2497061 (-) 2076 WP_071552301.1 type IV pilus secretin PilQ family protein Machinery gene
  BLL42_RS12080 (BLL42_11815) - 2497075..2497602 (-) 528 WP_071552302.1 pilus assembly protein PilP -
  BLL42_RS12085 (BLL42_11820) pilO 2497599..2498222 (-) 624 WP_071552303.1 type 4a pilus biogenesis protein PilO -
  BLL42_RS12090 (BLL42_11825) - 2498219..2498785 (-) 567 WP_071552304.1 PilN domain-containing protein -
  BLL42_RS12095 (BLL42_11830) pilM 2498785..2499849 (-) 1065 WP_071552305.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 691 a.a.        Molecular weight: 74882.16 Da        Isoelectric Point: 6.3696

>NTDB_id=203569 BLL42_RS12075 WP_071552301.1 2494986..2497061(-) (pilQ) [Pseudomonas frederiksbergensis strain ERDD5:01]
MNRIFSTLGFSLWMALLSPMVQAVNLKALDVAALPGDRVELKLAFDGPPPAPHGYTTDQPARIALDLPGVSSQLASKTRD
LGGGNARSATVVETKDRARLIINLTQLTPYSTRVEGNTLFVVVGQAAAARKPRPAAPRVASALPAPAKTYAPVSKAIRGV
DFQRGEQGEGNVVIDLSDPGISPDIQEHDGKIILNFAKTQLPERLRVRLDVKDFATPVQFVNASASSDKASISIEPSGTY
DYSTYQTDNKLTVSVRPMTVDDLQKRNAERFAYSGEKLSLNFQNIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQNVP
WDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESRKQIAELAPLRRELLQVNYAKAADIAKLFQSVTSAEAKV
DERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSVSNKGNWNASGVSSG
SSTTVGTPGSTSTNSPFVDMGAAGNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPYQ
EASSSGATSVAFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFSNT
QSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSH

Nucleotide


Download         Length: 2076 bp        

>NTDB_id=203569 BLL42_RS12075 WP_071552301.1 2494986..2497061(-) (pilQ) [Pseudomonas frederiksbergensis strain ERDD5:01]
ATGAACAGGATCTTCTCAACCCTCGGTTTTTCGCTATGGATGGCGCTGCTTTCTCCGATGGTCCAGGCAGTCAATCTCAA
AGCGCTGGATGTCGCCGCGCTGCCTGGTGATCGCGTCGAGTTGAAACTGGCTTTCGACGGGCCGCCGCCCGCGCCCCATG
GCTACACTACCGATCAGCCGGCCCGAATCGCCCTCGATTTGCCGGGCGTGAGCAGCCAACTGGCAAGCAAGACGCGGGAC
CTGGGAGGCGGCAACGCTCGCAGCGCGACGGTGGTCGAGACCAAGGACCGCGCCCGTTTGATCATCAACCTGACGCAGTT
GACGCCTTACAGCACACGAGTTGAGGGCAACACGCTGTTCGTGGTGGTTGGGCAGGCCGCCGCCGCTCGGAAACCCCGGC
CCGCTGCACCGCGAGTGGCCAGCGCCTTGCCAGCGCCAGCCAAGACTTATGCTCCCGTGAGCAAGGCCATTCGCGGCGTG
GACTTTCAGCGCGGTGAGCAGGGTGAAGGCAATGTCGTTATTGATTTATCGGACCCAGGTATCAGTCCGGACATCCAGGA
GCATGACGGCAAGATCATCCTCAACTTCGCCAAAACCCAGTTGCCTGAACGGTTGCGGGTTCGTCTGGACGTCAAGGATT
TCGCCACGCCGGTGCAGTTCGTCAACGCCAGTGCGTCTTCCGATAAAGCCAGCATCAGTATCGAGCCCAGTGGCACATAC
GACTATTCGACCTACCAGACTGACAACAAACTGACCGTCAGTGTCCGCCCGATGACCGTCGACGATCTGCAGAAGCGTAA
CGCAGAGCGCTTCGCCTACAGCGGCGAGAAGCTGTCGTTGAATTTTCAGAACATCGATGTGCGCTCGGTACTGCAACTGA
TCGCCGATTTCACCAACCTCAATCTGGTGGCCAGCGACACGGTGCAGGGCGGCATTACCTTGCGCCTGCAAAACGTGCCG
TGGGATCAGGCACTGGATCTGGTGCTCAAGACCAAAGGGTTGGACAAGCGCAAGATCGGCAACGTCTTGCTGGTAGCGCC
GGCAGATGAGATTGCCGCCCGTGAGCGCCAGGAGCTGGAGTCACGCAAACAGATCGCCGAGCTGGCGCCACTGCGCCGTG
AGCTGTTGCAGGTCAACTACGCCAAGGCGGCGGATATCGCCAAGCTGTTCCAGTCGGTGACCAGTGCCGAGGCGAAAGTC
GACGAGCGTGGTTCGATCACTGTCGACGAGCGAACCAACAACATTATTGCTTACCAGACCCAGGACCGTCTGGATGAACT
ACGGCGAATCGTCGCTCAACTGGATATTCCGGTGCGTCAGGTGATGATCGAGGCGCGGATTGTCGAAGCCAACGTCGACT
ACGATAAAAGCCTCGGCGTGCGTTGGGGCGGTTCGGTGAGTAACAAGGGTAACTGGAACGCTTCCGGGGTCAGCAGCGGC
TCTTCGACCACCGTCGGTACGCCGGGCAGCACCAGTACCAACTCACCGTTCGTCGACATGGGCGCGGCGGGGAATACGTC
GGGGATTGGCATCGCCTTCATCACCGATAACGTGTTGCTGGACCTTGAGCTGACGGCCATGGAGAAGACTGGCAACGGGG
AAATTGTCTCTCAACCCAAAGTGGTCACCTCCGACAAGGAAACCGCGAAGATCCTCAAAGGCACGGAAATTCCCTATCAG
GAAGCCAGCTCCAGCGGCGCGACATCGGTGGCGTTCAAGGAGGCCTCGCTGTCTCTGGAAGTGACACCACAGATCACCCC
GGACAACCGCATCATTATGGAGGTCAAGGTCACCAAGGATGAGCCGGACTATCTGAACAAAGTGCAGGATGTACCGCCGA
TCAAGAAGAATGAGGTTAACGCCAAGGTACTGGTCAACGATGGCGAAACCATCGTCATCGGCGGGGTTTTCTCAAATACT
CAAAGCAAGGTTGTAGATAAGGTGCCATTTCTCGGTGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGACGTGGTGTC
GGAGAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGAGTCATTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(375-440)

Bacterial type II and III secretion system protein (Secretin)

(526-682)

Secretin and TonB N terminus short domain (STN)

(301-347)

AMIN domain (AMIN)

(38-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1J0EKN8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.082

100

0.758
  comQ Acinetobacter baylyi ADP1

40.746

100

0.427
  pilQ Acinetobacter baumannii strain A118

38.247

100

0.417
  pilQ Acinetobacter baumannii D1279779

38.247

100

0.417
  pilQ Legionella pneumophila strain Lp02

38.667

97.685

0.378
  pilQ Legionella pneumophila strain ERS1305867

38.542

97.25

0.375

Multiple sequence alignment