Detailed information NTG0203570

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: BLL42_RS12095
• Genome accession: NZ_CP017886
• Coordinates: 2498785..2499849 (-)
• Length: 354 a.a.
• NCBI ID: WP_071552305.1
• Uniprot ID: A0A1J0EKL2
• Organism: Pseudomonas frederiksbergensis strain ERDD5:01
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2493785..2504849

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BLL42_RS12070 (BLL42_11805)	aroK	2494463..2494981 (-)	519	WP_071552300.1	shikimate kinase AroK	-
BLL42_RS12075 (BLL42_11810)	pilQ	2494986..2497061 (-)	2076	WP_071552301.1	type IV pilus secretin PilQ family protein	Machinery gene
BLL42_RS12080 (BLL42_11815)	-	2497075..2497602 (-)	528	WP_071552302.1	pilus assembly protein PilP	-
BLL42_RS12085 (BLL42_11820)	pilO	2497599..2498222 (-)	624	WP_071552303.1	type 4a pilus biogenesis protein PilO	-
BLL42_RS12090 (BLL42_11825)	-	2498219..2498785 (-)	567	WP_071552304.1	PilN domain-containing protein	-
BLL42_RS12095 (BLL42_11830)	pilM	2498785..2499849 (-)	1065	WP_071552305.1	pilus assembly protein PilM	Machinery gene
BLL42_RS12100 (BLL42_11835)	-	2500066..2502504 (+)	2439	WP_408004028.1	penicillin-binding protein 1A	-
BLL42_RS31040 (BLL42_11840)	-	2502578..2502787 (-)	210	WP_408004017.1	DUF6124 family protein	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38059.63 Da
• Isoelectric Point: 4.8142

>NTDB_id=203570 BLL42_RS12095 WP_071552305.1 2498785..2499849(-) (pilM) [Pseudomonas frederiksbergensis strain ERDD5:01]
MPGLFNKKSRTLLGIDISSTSVKLLELSRAGNRYRVEAYAVEPLPINAVVEKNIAELEGVGQALSRVLLKAKTSVKSVAV
AVAGSAVITKTIEMDAGLSADDMENQLKIEADQYIPYPLEEVAIDFEVQGVSRRNPERVDVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLTAQLAGVTERSTVAVVDIGATMTTLSVLSNGRIIYTREQLFGGRQLTEEIQRRYGMTV
EQAGLAKKQGGLPDDYVSEVLQPFREALVQQVSRSLQFFFASGQYNAVDYILLAGGTASVAGLDRLIEQRLGTPTQVANP
FADMTLSSKVNASALASDAPALMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=203570 BLL42_RS12095 WP_071552305.1 2498785..2499849(-) (pilM) [Pseudomonas frederiksbergensis strain ERDD5:01]
GTGCCAGGACTCTTCAATAAAAAAAGCCGCACGCTTTTGGGGATCGATATCAGCTCCACCTCGGTGAAGCTGCTTGAGTT
AAGCCGTGCAGGCAATCGATACCGGGTCGAAGCCTATGCGGTAGAGCCGCTGCCGATCAACGCGGTGGTCGAAAAGAATA
TCGCCGAACTGGAAGGTGTCGGACAGGCACTGTCCCGAGTGCTGCTCAAGGCCAAAACCAGCGTCAAAAGTGTCGCGGTG
GCCGTTGCCGGATCAGCGGTCATCACCAAGACCATCGAAATGGACGCCGGACTTTCTGCCGATGACATGGAAAATCAGCT
GAAGATCGAAGCTGACCAATACATTCCCTATCCCCTCGAAGAAGTCGCCATCGATTTTGAAGTGCAGGGCGTCTCCCGGC
GTAATCCCGAGCGTGTGGATGTACTGCTGGCGGCCTGTCGCAAGGAAAACGTCGAAGTTCGCGAAGCCGCGCTGGCTCTT
GCCGGCCTGACCGCACGGGTCGTCGATGTAGAGGCGTACGCGCTGGAGCGTTCATTCGGCTTGCTGACGGCACAGTTGGC
CGGTGTAACCGAGCGTTCGACGGTGGCCGTGGTCGACATCGGCGCCACCATGACCACCTTGAGCGTGTTGTCCAACGGAC
GAATCATTTACACCCGAGAGCAGTTGTTCGGCGGCCGTCAGTTGACGGAGGAAATCCAGCGTCGCTATGGCATGACCGTC
GAACAGGCCGGGCTTGCAAAAAAACAGGGTGGTTTGCCTGACGACTACGTCAGCGAAGTGCTGCAACCGTTCCGCGAGGC
GCTGGTGCAGCAGGTTTCGCGATCGTTGCAGTTCTTTTTCGCCTCGGGTCAGTACAACGCGGTCGACTACATCCTGCTGG
CCGGTGGCACCGCCTCGGTCGCCGGGCTGGATCGGCTGATCGAGCAGCGTCTGGGCACACCGACGCAGGTGGCCAATCCG
TTTGCGGACATGACATTGAGTAGCAAGGTCAACGCCAGTGCCCTGGCCAGTGATGCGCCGGCGCTGATGATTGCCTGTGG
CTTGGCGCTGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: A0A1J0EKL2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	54.416	99.153	0.54
comM	Acinetobacter nosocomialis M2	54.416	99.153	0.54
comM	Acinetobacter baylyi ADP1	54.261	99.435	0.54
pilM	Legionella pneumophila strain ERS1305867	46.045	100	0.46