Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   BKK79_RS13555 Genome accession   NZ_CP017748
Coordinates   3068058..3069794 (-) Length   578 a.a.
NCBI ID   WP_071015563.1    Uniprot ID   -
Organism   Cupriavidus sp. USMAA2-4 isolate pure     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3063058..3074794
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BKK79_RS13525 (BKK79_13525) - 3063469..3063897 (+) 429 WP_071015573.1 NUDIX domain-containing protein -
  BKK79_RS13530 (BKK79_13530) - 3063938..3064129 (-) 192 WP_071015571.1 DNA gyrase inhibitor YacG -
  BKK79_RS13535 (BKK79_13535) zapD 3064214..3064972 (-) 759 WP_071016592.1 cell division protein ZapD -
  BKK79_RS13540 (BKK79_13540) coaE 3065180..3065812 (-) 633 WP_071015570.1 dephospho-CoA kinase -
  BKK79_RS13545 (BKK79_13545) pilD 3065867..3066760 (-) 894 WP_071037872.1 A24 family peptidase Machinery gene
  BKK79_RS13550 (BKK79_13550) - 3066764..3068029 (-) 1266 WP_071037873.1 type II secretion system F family protein -
  BKK79_RS13555 (BKK79_13555) pilF 3068058..3069794 (-) 1737 WP_071015563.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BKK79_RS13565 (BKK79_13565) ispB 3070164..3071093 (-) 930 WP_071015561.1 octaprenyl diphosphate synthase -
  BKK79_RS13570 (BKK79_13570) rplU 3071738..3072049 (+) 312 WP_006576525.1 50S ribosomal protein L21 -
  BKK79_RS13575 (BKK79_13575) rpmA 3072110..3072370 (+) 261 WP_071015560.1 50S ribosomal protein L27 -
  BKK79_RS13580 (BKK79_13580) obgE 3072513..3073613 (+) 1101 WP_071015558.1 GTPase ObgE -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 63322.88 Da        Isoelectric Point: 7.8029

>NTDB_id=202501 BKK79_RS13555 WP_071015563.1 3068058..3069794(-) (pilF) [Cupriavidus sp. USMAA2-4 isolate pure]
MTLGLALAQSRRIAPALLAQLEQAAREKKSQLIDEIVGSGTMSARDLATFAADKYQLPLLDLSQFNFATVPPALVGNREF
HAHRLLPLGRRDNRLVVATSDPSNQAGLDAIRQKLNLPVDAVVVEHDKLLKHVRSAGEESSTLRAAGPVAAEHKLIEYDP
VASAGAQRNKAPNDIDDAPVVRFLHKLLTEAFHRGASDLHFEPFETFYRVRFRVDGVLLEVARPPLDIRDKIATRIKVLS
RLDISEKRVPQDGRMKLLIALPKEKDKDGKDSKETVEKAIDFRVSTLPTLFGEKIVMRILDSSTDKLDIDRLGYEPAQKA
LLLETIKRPYGMILVTGPTGSGKTVSLYTFLNLLNQGDINISTAEDPAEIQLPGINQVNVNEKAGLTFAAALRSFLRQDP
DVIMVGEIRDLETADIAIKAAQTGHLVLSTLHTNDAPTTLTRLMNMGVAPFNIASSVLLITAQRLARRLCNCKRPGALAR
DVLLDAGFQESELDGSWQPYHPAGCERCSGSGYKGRCGIYQVMPISEAMQQIILSHGTALQIAEQARRDGVLSLREAGLL
KVKQGVTSLEEVLATTNM

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=202501 BKK79_RS13555 WP_071015563.1 3068058..3069794(-) (pilF) [Cupriavidus sp. USMAA2-4 isolate pure]
ATGACACTAGGTCTCGCTCTCGCGCAAAGCCGCCGCATCGCTCCGGCCCTTCTCGCCCAGCTCGAACAGGCCGCGCGGGA
AAAGAAGTCGCAACTGATCGACGAGATCGTCGGCAGCGGCACCATGAGCGCGCGCGACCTCGCCACCTTCGCCGCCGACA
AGTACCAGCTCCCGCTGCTGGACCTCAGCCAGTTCAACTTCGCCACGGTGCCGCCGGCCCTGGTCGGCAACCGCGAGTTC
CACGCCCACCGGCTGCTGCCGCTGGGCCGGCGCGACAACCGGCTGGTCGTGGCAACCTCCGATCCGTCCAACCAGGCCGG
CCTCGATGCCATCCGCCAGAAACTGAACCTGCCGGTCGACGCGGTCGTGGTCGAGCACGACAAGCTGCTGAAGCACGTGC
GCTCGGCCGGCGAGGAAAGCAGTACGCTGCGCGCCGCCGGCCCGGTGGCGGCCGAGCACAAGCTGATCGAGTACGACCCG
GTCGCCTCGGCGGGAGCCCAGCGCAACAAGGCGCCCAACGACATCGACGACGCACCGGTCGTGCGCTTCCTGCACAAGCT
GCTGACCGAGGCCTTCCACCGGGGCGCATCCGACCTGCACTTCGAGCCGTTCGAGACCTTCTACCGCGTGCGCTTCCGCG
TCGACGGCGTGCTGCTGGAGGTGGCCCGCCCCCCGCTGGACATCCGCGACAAGATCGCCACCCGCATCAAGGTGCTGTCG
CGCCTGGACATCTCCGAGAAACGCGTGCCGCAGGACGGCCGCATGAAGCTGCTGATCGCGCTGCCGAAGGAAAAGGACAA
GGACGGCAAGGACAGCAAGGAGACGGTCGAGAAGGCCATCGATTTCCGTGTGTCGACGCTGCCGACACTGTTCGGCGAGA
AGATCGTCATGCGGATCCTCGACTCCTCGACCGACAAGCTCGACATCGACCGGCTCGGCTACGAGCCCGCGCAGAAGGCG
CTGCTGCTCGAGACCATCAAGCGCCCCTACGGCATGATCCTGGTCACCGGCCCGACCGGCAGCGGCAAGACGGTCTCGCT
CTACACCTTCCTCAACCTGCTCAACCAGGGCGACATCAATATCTCGACGGCCGAGGATCCCGCCGAAATCCAGCTGCCCG
GCATCAACCAGGTCAACGTCAACGAGAAGGCCGGCCTGACCTTCGCCGCCGCGCTGCGCTCCTTCCTGCGCCAGGACCCG
GACGTCATCATGGTGGGCGAGATCCGCGACCTGGAGACCGCGGACATCGCCATCAAGGCCGCCCAGACCGGCCATCTGGT
GCTGTCCACGCTGCATACCAACGATGCGCCGACCACGCTGACGCGGCTGATGAACATGGGCGTGGCGCCGTTCAACATCG
CCTCCAGCGTGCTGCTGATCACCGCCCAGCGGCTGGCGCGCCGGCTGTGCAACTGCAAGCGGCCGGGCGCCCTGGCGCGC
GACGTGCTGCTTGACGCCGGCTTCCAAGAGAGCGAACTCGACGGCAGCTGGCAGCCTTACCACCCGGCCGGCTGCGAGCG
CTGCAGCGGCAGCGGCTACAAGGGGCGTTGCGGCATCTACCAGGTGATGCCCATCTCCGAGGCGATGCAGCAGATCATCC
TGTCTCACGGTACGGCGCTGCAGATCGCCGAGCAGGCGCGCCGCGACGGCGTGCTATCGTTGCGGGAAGCCGGCCTGCTG
AAGGTGAAGCAAGGCGTCACCTCGCTGGAAGAAGTGCTGGCGACGACCAATATGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(39-136)

Type II/IV secretion system protein (T2SSE)

(186-468)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

50.853

100

0.516
  pilB Acinetobacter baumannii D1279779

50.685

100

0.512
  pilB Acinetobacter baylyi ADP1

51.434

96.54

0.497
  pilB Legionella pneumophila strain ERS1305867

49.464

96.886

0.479
  pilB Vibrio cholerae strain A1552

46.367

100

0.464
  pilB Vibrio parahaemolyticus RIMD 2210633

47.145

93.945

0.443
  pilB Vibrio campbellii strain DS40M4

45.173

94.983

0.429
  pilF Thermus thermophilus HB27

38.382

100

0.386
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.188

97.405

0.372

Multiple sequence alignment