Detailed information NTG0202500

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: BKK79_RS13545
• Genome accession: NZ_CP017748
• Coordinates: 3065867..3066760 (-)
• Length: 297 a.a.
• NCBI ID: WP_071037872.1
• Uniprot ID: A0A1D9GYH2
• Organism: Cupriavidus sp. USMAA2-4 isolate pure
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3060867..3071760

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BKK79_RS13515 (BKK79_13515)	argJ	3061316..3062542 (+)	1227	WP_071037871.1	bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ	-
BKK79_RS13520 (BKK79_13520)	-	3062579..3063469 (+)	891	WP_071015574.1	ATP-binding protein	-
BKK79_RS13525 (BKK79_13525)	-	3063469..3063897 (+)	429	WP_071015573.1	NUDIX domain-containing protein	-
BKK79_RS13530 (BKK79_13530)	-	3063938..3064129 (-)	192	WP_071015571.1	DNA gyrase inhibitor YacG	-
BKK79_RS13535 (BKK79_13535)	zapD	3064214..3064972 (-)	759	WP_071016592.1	cell division protein ZapD	-
BKK79_RS13540 (BKK79_13540)	coaE	3065180..3065812 (-)	633	WP_071015570.1	dephospho-CoA kinase	-
BKK79_RS13545 (BKK79_13545)	pilD	3065867..3066760 (-)	894	WP_071037872.1	A24 family peptidase	Machinery gene
BKK79_RS13550 (BKK79_13550)	-	3066764..3068029 (-)	1266	WP_071037873.1	type II secretion system F family protein	-
BKK79_RS13555 (BKK79_13555)	pilF	3068058..3069794 (-)	1737	WP_071015563.1	type IV-A pilus assembly ATPase PilB	Machinery gene
BKK79_RS13565 (BKK79_13565)	ispB	3070164..3071093 (-)	930	WP_071015561.1	octaprenyl diphosphate synthase	-

Sequence

Protein

• Length: 297 a.a.
• Molecular weight: 31687.69 Da
• Isoelectric Point: 7.8053

>NTDB_id=202500 BKK79_RS13545 WP_071037872.1 3065867..3066760(-) (pilD) [Cupriavidus sp. USMAA2-4 isolate pure]
MVSAFSPQGAFTIATLAALPGWFLMLAAGLLGLAVGSFLNVVIHRLPRMMEHDEANYIAELRGEPLPHPSRYNLAVPGSA
CPHCGHTLAPWENIPVLSYLLLRGRCSACHAPIGARYPLVELASGVLSVLAAWHFGPTWQGLAALVLVWMLLALTMIDAD
TQLLPDQLTQPLLWLGLLLNLAGVFASLADAVIGAAAGYLLLWSVYWLFKLVRGKEGMGYGDFKLMAALGAWFGWQALPA
LVLLSSVVGLLFGLVRIALRRQHRDTPFPFGPFIAGAGLLVLFLGAGALPLALGVPG

Nucleotide

• Length: 894 bp

>NTDB_id=202500 BKK79_RS13545 WP_071037872.1 3065867..3066760(-) (pilD) [Cupriavidus sp. USMAA2-4 isolate pure]
ATGGTGTCCGCATTCTCGCCGCAAGGCGCCTTCACCATCGCCACGCTGGCCGCCCTGCCCGGCTGGTTCCTGATGCTGGC
GGCCGGCCTGCTGGGCCTGGCGGTCGGCAGCTTCCTCAACGTGGTGATCCACCGCCTGCCGCGCATGATGGAGCACGACG
AGGCGAACTACATCGCCGAGCTGCGCGGCGAGCCGCTGCCCCATCCGTCGCGCTACAACCTGGCGGTGCCGGGATCGGCA
TGCCCGCACTGCGGGCACACGCTGGCGCCATGGGAGAACATCCCCGTGCTCAGCTACCTGCTGCTGCGCGGGCGCTGTTC
GGCCTGCCACGCACCGATCGGGGCGCGCTATCCGCTGGTGGAACTCGCCAGCGGGGTGCTGAGCGTGCTGGCCGCCTGGC
ACTTCGGGCCGACCTGGCAGGGCCTGGCGGCGCTGGTGCTGGTGTGGATGCTGCTGGCGCTGACCATGATCGACGCCGAT
ACCCAGCTGCTGCCCGACCAGCTGACCCAGCCGCTGCTGTGGCTCGGCCTGCTGCTCAACCTGGCGGGCGTGTTCGCCTC
GCTGGCCGACGCCGTGATCGGCGCGGCAGCCGGCTACCTGCTGCTGTGGAGCGTCTACTGGCTCTTCAAGCTGGTGCGCG
GCAAGGAGGGCATGGGCTACGGCGACTTCAAGCTGATGGCGGCGCTCGGTGCCTGGTTCGGCTGGCAAGCGCTGCCCGCG
CTGGTGCTGCTGTCCTCCGTGGTCGGCCTGCTGTTCGGCCTCGTCCGCATCGCACTGCGCCGGCAGCATCGCGACACGCC
CTTCCCCTTCGGTCCGTTCATCGCCGGAGCGGGCCTGCTGGTACTGTTCCTGGGGGCCGGCGCGCTGCCGCTCGCGCTGG
GCGTGCCGGGCTAG

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (145-254)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (30-135)

3D structure

• AlphaFold DB: A0A1D9GYH2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	54.307	89.899	0.488
pilD	Vibrio campbellii strain DS40M4	53.933	89.899	0.485
pilD	Acinetobacter baumannii D1279779	46.69	96.633	0.451
pilD	Acinetobacter nosocomialis M2	46.341	96.633	0.448
pilD	Neisseria gonorrhoeae MS11	49.027	86.532	0.424