Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   Spy49_0095 Genome accession   CP000829
Coordinates   105145..105579 (+) Length   144 a.a.
NCBI ID   ACI60448.1    Uniprot ID   -
Organism   Streptococcus pyogenes NZ131     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 100145..110579
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Spy49_0089 - 101814..102179 (+) 366 ACI60442.1 Putative DNA binding protein -
  Spy49_0090 comGA 102272..103210 (+) 939 ACI60443.1 Putative ABC transporter subunit ComYA Machinery gene
  Spy49_0091 comGB 103305..104180 (+) 876 ACI60444.1 Putative competence protein, ABC transporter subunit Machinery gene
  Spy49_0092 comGC 104182..104508 (+) 327 ACI60445.1 Putative competence protein Machinery gene
  Spy49_0093 comGD 104483..104911 (+) 429 ACI60446.1 Competence protein Machinery gene
  Spy49_0094 comGE 104868..105152 (+) 285 ACI60447.1 hypothetical protein Machinery gene
  Spy49_0095 comGF 105145..105579 (+) 435 ACI60448.1 Putative competence protein Machinery gene
  Spy49_0096 - 105599..105889 (+) 291 ACI60449.1 hypothetical protein -
  Spy49_0097 comGH 105987..106940 (+) 954 ACI60450.1 Adenine-specific methyltransferase Machinery gene
  Spy49_0098 ackA 106999..108195 (+) 1197 ACI60451.1 Acetate kinase -
  Spy49_0099 - 108381..108623 (+) 243 ACI60452.1 hypothetical protein -
  Spy49_0100 proC 108772..109542 (-) 771 ACI60453.1 Pyrroline-5-carboxylate reductase -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16687.32 Da        Isoelectric Point: 10.0822

>NTDB_id=20230 Spy49_0095 ACI60448.1 105145..105579(+) (comGF) [Streptococcus pyogenes NZ131]
MSKQLSNIKAFTLLEALIALLVISGSLLVYQGLTQTLLKRSHYLARHDQDNWLLFSHQLREELSEARFCKVADNKLYVEK
GKKVLAFGQFKSHDFRKSASNGKGYQPMLFGISRSHIHIEQSQICITLKWKSGLERTFYYTFQD

Nucleotide


Download         Length: 435 bp        

>NTDB_id=20230 Spy49_0095 ACI60448.1 105145..105579(+) (comGF) [Streptococcus pyogenes NZ131]
TTGAGTAAACAATTAAGTAACATAAAAGCTTTTACCCTTCTAGAGGCGTTAATAGCCTTACTCGTGATATCAGGGTCTTT
ATTGGTTTATCAAGGTTTGACCCAAACCCTCCTTAAACGTAGCCATTACCTAGCCCGTCATGATCAAGACAATTGGCTCT
TATTTTCTCATCAATTGCGAGAGGAGTTAAGTGAAGCAAGATTTTGCAAAGTAGCTGATAATAAACTATACGTTGAAAAA
GGAAAGAAAGTACTAGCTTTTGGCCAATTTAAAAGTCATGATTTTCGAAAATCAGCTAGTAATGGAAAAGGATATCAACC
CATGTTATTTGGAATATCACGTAGTCATATTCACATAGAGCAGTCACAGATTTGCATTACTTTAAAGTGGAAAAGTGGGT
TAGAAAGGACTTTTTATTATACCTTTCAAGACTAA

Domains

Predicted by InterProScan.

Putative Competence protein ComGF (ComGF)

(41-135)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Streptococcus mutans UA140

49.64

96.528

0.479
  comGF Streptococcus mutans UA159

48.921

96.528

0.472
  comGF Lactococcus lactis subsp. cremoris KW2

45.985

95.139

0.437
  comGF/cglF Streptococcus mitis NCTC 12261

44.776

93.056

0.417
  comGF/cglF Streptococcus pneumoniae Rx1

43.609

92.361

0.403
  comGF/cglF Streptococcus pneumoniae D39

43.609

92.361

0.403
  comGF/cglF Streptococcus pneumoniae R6

43.609

92.361

0.403
  comGF/cglF Streptococcus pneumoniae TIGR4

43.609

92.361

0.403
  comGF/cglF Streptococcus mitis SK321

42.857

92.361

0.396