Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BKP64_RS02380 Genome accession   NZ_CP017715
Coordinates   499085..500800 (-) Length   571 a.a.
NCBI ID   WP_070965515.1    Uniprot ID   A0A1D9GHJ7
Organism   Marinobacter salinus strain Hb8     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 494085..505800
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BKP64_RS02355 (BKP64_02355) tsaA 494469..495212 (-) 744 WP_070965503.1 tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO -
  BKP64_RS02360 (BKP64_02360) - 495223..496263 (-) 1041 WP_099092572.1 hypothetical protein -
  BKP64_RS02365 (BKP64_02365) coaE 496307..496906 (-) 600 WP_070965506.1 dephospho-CoA kinase -
  BKP64_RS02370 (BKP64_02370) pilD 496910..497785 (-) 876 WP_070965509.1 prepilin peptidase Machinery gene
  BKP64_RS02375 (BKP64_02375) pilC 497862..499082 (-) 1221 WP_070965513.1 type II secretion system F family protein Machinery gene
  BKP64_RS02380 (BKP64_02380) pilB 499085..500800 (-) 1716 WP_070965515.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BKP64_RS02385 (BKP64_02385) - 501197..501697 (+) 501 WP_083329275.1 pilin -
  BKP64_RS02390 (BKP64_02390) - 502024..502467 (+) 444 WP_070965522.1 pilin -
  BKP64_RS02395 (BKP64_02395) - 502524..503885 (-) 1362 WP_070965525.1 MBL fold metallo-hydrolase -
  BKP64_RS02400 (BKP64_02400) cysQ 503976..504743 (+) 768 WP_070965528.1 3'(2'),5'-bisphosphate nucleotidase CysQ -
  BKP64_RS02405 (BKP64_02405) rfaH 504740..505219 (+) 480 WP_070965531.1 transcription/translation regulatory transformer protein RfaH -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62736.83 Da        Isoelectric Point: 4.9101

>NTDB_id=202191 BKP64_RS02380 WP_070965515.1 499085..500800(-) (pilB) [Marinobacter salinus strain Hb8]
MSTNKSNITLTGLARRFVDDGLLDEATAKDAFLQASQNRIPLITYLTQNNLANSSKLAFSAAMEFGVSVLDLSSFMPEMM
PEKAVDEKLVRKHNALPLYKRGNRLFIAVSDPTNIQALDEIKFNTGLSTDAILVDDAKLREAIDKYLESQDTSMGDLDDA
DLEGVETEGGEQEDDAAISASEVDDAPIVKYVNKMLLDAIRGGASDVHFEPYEKVYRVRYRTDGILKEVSRPSIKLAPKI
SARVKIMAQLDISERRVPQDGRIKMKLSKTKAIDFRVNTLPTLWGEKIVLRILDPSQAKLGIDVLGYEEDQKKLYMDALE
QPQGMILVTGPTGSGKTVSLYTGLNILNTPGINISTAEDPAEINLEGINQVNVNTRVGLGFAEALRAFLRQDPDVIMVGE
IRDLETANIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPSFNIATSVSLIIAQRLGRRLCSSCKQPAEIPKDVLLEE
GFTQEQIDTGFTLFRPKGCDKCNGGYKGRVGIYEVVKITDELANMIMEEASSIKIAKQAQAEGFRNLRQAALLKVMEGVT
SLEEANRVTKD

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=202191 BKP64_RS02380 WP_070965515.1 499085..500800(-) (pilB) [Marinobacter salinus strain Hb8]
ATGTCTACCAATAAAAGCAATATCACGCTCACAGGCCTGGCACGGCGCTTTGTGGATGACGGACTTCTGGACGAAGCAAC
CGCCAAGGATGCTTTCCTTCAAGCCTCCCAGAACCGAATTCCCCTGATCACTTATCTTACCCAGAACAATCTGGCAAACA
GCTCCAAGCTGGCGTTTTCTGCAGCTATGGAGTTTGGCGTCTCAGTGCTTGATTTATCATCATTTATGCCCGAGATGATG
CCTGAGAAGGCGGTGGACGAAAAGCTGGTTCGCAAGCACAACGCGCTTCCTCTATATAAAAGAGGCAACCGTCTGTTCAT
TGCAGTATCGGACCCCACCAACATCCAGGCGTTGGACGAGATCAAGTTCAACACAGGCCTGAGCACCGACGCCATTCTGG
TCGACGACGCCAAGCTGCGGGAGGCTATCGATAAGTACCTTGAGTCCCAGGATACGTCCATGGGGGACCTGGACGATGCG
GATCTGGAAGGTGTAGAAACGGAAGGTGGCGAACAGGAAGACGATGCTGCCATATCGGCAAGTGAAGTTGACGATGCACC
TATCGTCAAATACGTGAATAAAATGTTGCTAGATGCCATTCGCGGCGGCGCATCGGATGTCCACTTTGAGCCTTATGAAA
AGGTCTACCGGGTTCGTTATCGCACCGACGGCATTCTGAAAGAGGTTTCCCGCCCTTCCATAAAGCTGGCTCCCAAAATC
TCAGCTCGTGTAAAAATCATGGCGCAGCTGGACATTTCCGAACGCCGGGTTCCCCAGGACGGCCGGATCAAGATGAAACT
GTCCAAGACCAAAGCCATCGATTTCCGGGTCAACACCCTGCCCACGCTGTGGGGTGAGAAAATCGTTTTACGGATATTGG
ACCCAAGTCAGGCCAAGCTGGGCATTGATGTGCTGGGCTATGAGGAAGATCAGAAGAAATTGTATATGGATGCCCTGGAA
CAGCCACAAGGCATGATTCTGGTGACGGGGCCCACCGGTTCCGGTAAAACCGTTTCTCTCTATACCGGTTTGAACATTCT
CAATACCCCAGGTATCAACATATCCACGGCGGAAGACCCTGCAGAAATAAACCTGGAAGGTATCAACCAGGTAAACGTGA
ATACGCGGGTGGGACTAGGCTTTGCCGAAGCGCTTCGTGCATTCCTACGCCAGGACCCGGATGTGATCATGGTGGGTGAG
ATCCGGGACCTTGAAACCGCAAACATTGCCATCAAGGCCGCCCAAACAGGCCACTTGGTTCTTTCCACCCTGCACACCAA
CAGCGCTGCAGAGACGCTTACCCGGATGATGAACATGGGCGTTCCCTCGTTCAACATTGCTACATCCGTAAGCCTGATTA
TTGCCCAGCGACTTGGTCGGCGCCTGTGCAGCAGCTGCAAACAACCGGCAGAGATTCCAAAAGACGTACTTTTGGAGGAA
GGGTTCACACAGGAACAAATTGATACAGGGTTCACGTTGTTCCGCCCCAAGGGCTGTGATAAATGCAATGGAGGATATAA
GGGCCGCGTCGGTATTTACGAGGTGGTCAAGATTACCGACGAGCTGGCGAATATGATTATGGAAGAAGCCAGTTCCATTA
AAATTGCAAAGCAGGCTCAGGCAGAAGGCTTCCGTAATTTGCGTCAGGCAGCACTGTTAAAAGTGATGGAGGGCGTGACG
AGCCTTGAGGAAGCCAACCGCGTGACGAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-152)

Type II/IV secretion system protein (T2SSE)

(194-461)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1D9GHJ7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.592

100

0.578
  pilB Acinetobacter baylyi ADP1

56.818

100

0.569
  pilB Legionella pneumophila strain ERS1305867

53.71

99.124

0.532
  pilB Vibrio parahaemolyticus RIMD 2210633

51.805

97.023

0.503
  pilB Vibrio campbellii strain DS40M4

50.177

98.774

0.496
  pilB Vibrio cholerae strain A1552

53.385

90.543

0.483
  pilF Neisseria gonorrhoeae MS11

48.41

99.124

0.48
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.114

92.119

0.37
  pilB/pilB1 Synechocystis sp. PCC 6803

39.961

90.718

0.363

Multiple sequence alignment