Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   BKP64_RS02370 Genome accession   NZ_CP017715
Coordinates   496910..497785 (-) Length   291 a.a.
NCBI ID   WP_070965509.1    Uniprot ID   A0A1D9GHJ3
Organism   Marinobacter salinus strain Hb8     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 491910..502785
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BKP64_RS02350 (BKP64_02350) - 492074..494425 (-) 2352 WP_070965496.1 response regulator -
  BKP64_RS02355 (BKP64_02355) tsaA 494469..495212 (-) 744 WP_070965503.1 tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO -
  BKP64_RS02360 (BKP64_02360) - 495223..496263 (-) 1041 WP_099092572.1 hypothetical protein -
  BKP64_RS02365 (BKP64_02365) coaE 496307..496906 (-) 600 WP_070965506.1 dephospho-CoA kinase -
  BKP64_RS02370 (BKP64_02370) pilD 496910..497785 (-) 876 WP_070965509.1 prepilin peptidase Machinery gene
  BKP64_RS02375 (BKP64_02375) pilC 497862..499082 (-) 1221 WP_070965513.1 type II secretion system F family protein Machinery gene
  BKP64_RS02380 (BKP64_02380) pilB 499085..500800 (-) 1716 WP_070965515.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BKP64_RS02385 (BKP64_02385) - 501197..501697 (+) 501 WP_083329275.1 pilin -
  BKP64_RS02390 (BKP64_02390) - 502024..502467 (+) 444 WP_070965522.1 pilin -

Sequence

Protein


Download         Length: 291 a.a.        Molecular weight: 32242.50 Da        Isoelectric Point: 7.1816

>NTDB_id=202189 BKP64_RS02370 WP_070965509.1 496910..497785(-) (pilD) [Marinobacter salinus strain Hb8]
MPTLDVFLATPWLLYLSVILVSLCIGSFLNVVILRLPKMMHQDWRCQCEEFLELPEQQRKQEAIVTLSKPASTCPSCGHS
IRAWENIPVISYLVLGGKCSSCKTRISPRYPIIEAVTALFSVITVLLLGPTEAALWALLLVWSLVALTVIDFDTQLLPDN
ITLPLLWLGLVLNYFGVLTDFTSAFWGAVAGYLSLWSVYWLFKLVTGKEGMGHGDFKLLAALGAWLGWQLLPAVILLSSV
VGAIVGISLMIFKKHGREVPIPFGPYLAAAGLLCLWFGPEIQRFWFGFLGV

Nucleotide


Download         Length: 876 bp        

>NTDB_id=202189 BKP64_RS02370 WP_070965509.1 496910..497785(-) (pilD) [Marinobacter salinus strain Hb8]
ATGCCTACTCTTGATGTGTTTCTGGCCACGCCCTGGCTTCTGTACCTCTCCGTAATTCTTGTTTCTCTCTGCATCGGCAG
TTTCCTCAACGTCGTCATCCTTCGCCTGCCAAAAATGATGCACCAGGACTGGCGTTGCCAGTGTGAGGAGTTTCTGGAGC
TTCCGGAACAACAACGGAAACAGGAGGCCATCGTTACCCTTTCAAAACCCGCTTCAACCTGCCCTTCCTGTGGTCACAGC
ATTCGGGCCTGGGAAAATATTCCTGTAATCAGCTACCTGGTTCTTGGCGGTAAATGCTCATCCTGCAAAACCCGGATTTC
GCCCCGGTACCCGATTATTGAAGCGGTAACGGCGCTCTTTTCCGTTATCACGGTTCTGCTGCTGGGCCCCACCGAAGCGG
CGCTTTGGGCATTGCTGCTGGTTTGGTCGCTGGTTGCTTTAACAGTTATTGATTTCGACACCCAACTGCTGCCGGACAAC
ATCACCCTGCCCCTGCTCTGGCTGGGCCTGGTGCTGAACTATTTCGGCGTGTTGACGGATTTCACCAGTGCCTTCTGGGG
CGCCGTTGCCGGCTATCTTTCCCTCTGGTCTGTCTACTGGCTGTTCAAACTGGTGACCGGCAAGGAGGGCATGGGGCACG
GCGACTTCAAGTTGCTGGCAGCTCTGGGCGCCTGGTTGGGCTGGCAGCTTTTGCCGGCGGTGATTCTCCTTTCGTCCGTG
GTTGGCGCTATCGTTGGCATCAGTCTCATGATCTTCAAAAAGCACGGCCGTGAAGTTCCAATTCCTTTCGGGCCCTACCT
GGCCGCAGCTGGCCTGCTGTGCTTGTGGTTTGGTCCTGAAATTCAGCGTTTCTGGTTTGGCTTTCTGGGTGTTTGA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(138-247)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-126)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1D9GHJ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Acinetobacter baumannii D1279779

55.474

94.158

0.522
  pilD Acinetobacter nosocomialis M2

55.109

94.158

0.519
  pilD Vibrio cholerae strain A1552

51.228

97.938

0.502
  pilD Vibrio campbellii strain DS40M4

50.175

97.938

0.491
  pilD Neisseria gonorrhoeae MS11

47.191

91.753

0.433

Multiple sequence alignment