Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BJP62_RS14500 Genome accession   NZ_CP017668
Coordinates   3057726..3059432 (-) Length   568 a.a.
NCBI ID   WP_070530704.1    Uniprot ID   A0A1D9BH38
Organism   Jeongeupia sp. USM3     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3052726..3064432
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BJP62_RS14470 (BJP62_14470) - 3053141..3053893 (-) 753 WP_070530697.1 GNAT family N-acetyltransferase -
  BJP62_RS14475 (BJP62_14475) - 3053978..3054163 (-) 186 WP_145927214.1 DNA gyrase inhibitor YacG -
  BJP62_RS14480 (BJP62_14480) zapD 3054160..3054918 (-) 759 WP_070530698.1 cell division protein ZapD -
  BJP62_RS14485 (BJP62_14485) coaE 3054985..3055599 (-) 615 WP_070530700.1 dephospho-CoA kinase -
  BJP62_RS14490 (BJP62_14490) pilD 3055602..3056468 (-) 867 WP_070532784.1 A24 family peptidase Machinery gene
  BJP62_RS14495 (BJP62_14495) pilC 3056474..3057697 (-) 1224 WP_070530702.1 type II secretion system F family protein Machinery gene
  BJP62_RS14500 (BJP62_14500) pilB 3057726..3059432 (-) 1707 WP_070530704.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BJP62_RS14505 (BJP62_14505) - 3059548..3060804 (-) 1257 WP_070530706.1 HlyC/CorC family transporter -
  BJP62_RS14510 (BJP62_14510) - 3060846..3061652 (-) 807 WP_070530707.1 inner membrane protein YpjD -
  BJP62_RS14515 (BJP62_14515) ffh 3061734..3063083 (+) 1350 WP_070530709.1 signal recognition particle protein -
  BJP62_RS14520 (BJP62_14520) rpsP 3063183..3063434 (+) 252 WP_070530711.1 30S ribosomal protein S16 -
  BJP62_RS14525 (BJP62_14525) rimM 3063493..3063975 (+) 483 WP_070530713.1 ribosome maturation factor RimM -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 61871.89 Da        Isoelectric Point: 5.1994

>NTDB_id=201404 BJP62_RS14500 WP_070530704.1 3057726..3059432(-) (pilB) [Jeongeupia sp. USM3]
MSTTPSISGLARLLVQHGRLSDTDAEALQGAAATNKTPFIAQLLQSNKISAKEVAEFSSQAFGYPLLDLDQIDPSYIPQG
MLDTKVMQAQRIVPLFKRGARLFVGVSDITNSQAAEEVRFQTGSQIEPIVVEDNKLVKLLDQLLEASGATLKNLEMDESE
LEISGGDPEPQNDSANIDVDDAPVVKYLQKILLDAINAGASDIHFEPYEKYYRIRYRQDGILREIAQPPLAIKEKIASRI
KVISKLDISEKRVPQDGRMKLVLSKSRAIDFRVSTLPTLHGEKIVMRILDPTSATLGIDALGYEPDQKAALLDAVSRPYG
MVLVTGPTGSGKTVSLYTCLNILNEPGTNISTAEDPAEINLPGVNQVNVNDKAGLTFSAALKAFLRQDPDIIMVGEIRDL
ETADIAIKAAQTGHMVFSTLHTNDAPGTLTRLLNMGVAPFNVASSVILITAQRLARKLCTQCKTPADIPDEALLRAGFTE
AQLDGSWRPYKAVGCDHCKGTGYKGRVGIYQVMPISDEMNRLIMKNGTSIDLADQARREGVRDLRQSGLLKVKQGMTSLE
EIMAVTNE

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=201404 BJP62_RS14500 WP_070530704.1 3057726..3059432(-) (pilB) [Jeongeupia sp. USM3]
ATGTCCACTACGCCTTCGATTTCCGGCCTGGCCCGCCTGCTGGTCCAGCATGGCCGACTCAGCGACACCGACGCCGAAGC
GCTGCAAGGCGCCGCGGCGACCAACAAGACGCCGTTCATCGCCCAGCTGCTGCAAAGCAACAAGATCAGCGCCAAGGAAG
TCGCCGAATTCAGCTCGCAGGCCTTCGGCTATCCGCTGCTCGACCTCGACCAGATCGACCCCAGCTACATTCCGCAGGGC
ATGCTCGACACCAAGGTGATGCAGGCGCAGCGCATCGTCCCGCTGTTCAAGCGCGGCGCGCGGCTCTTCGTCGGCGTCTC
CGATATCACCAATTCGCAGGCCGCCGAGGAAGTCCGCTTCCAGACCGGCAGCCAGATCGAGCCGATCGTCGTCGAGGACA
ACAAGCTCGTCAAACTGCTCGACCAGTTGCTCGAGGCGTCCGGCGCCACGCTGAAGAATCTCGAGATGGACGAGTCCGAG
CTCGAGATCAGCGGCGGCGACCCCGAACCGCAGAACGATTCGGCGAACATCGACGTCGACGACGCGCCGGTCGTCAAATA
CCTGCAGAAAATCCTGCTCGACGCGATCAACGCCGGCGCGTCGGACATCCATTTCGAGCCGTACGAGAAGTACTACCGGA
TCCGCTACCGCCAGGACGGCATCCTGCGCGAAATCGCCCAGCCGCCGCTGGCAATCAAGGAAAAGATCGCCTCGCGGATC
AAGGTGATCTCCAAGCTCGACATCTCCGAAAAACGCGTCCCGCAGGACGGCCGGATGAAGCTGGTGCTGTCGAAAAGCCG
CGCGATCGACTTCCGTGTCTCGACGCTGCCGACGCTGCACGGCGAAAAGATCGTCATGCGGATTCTCGACCCGACATCGG
CGACGCTCGGCATCGATGCGCTCGGTTACGAGCCGGACCAGAAGGCCGCACTGCTCGACGCCGTCAGCCGCCCCTACGGC
ATGGTACTGGTCACCGGCCCGACCGGCTCGGGCAAGACCGTCTCGCTGTACACCTGCCTGAACATCCTCAACGAGCCCGG
CACCAATATCTCGACCGCCGAGGACCCGGCCGAAATCAACCTGCCCGGCGTCAACCAGGTCAACGTCAACGACAAGGCCG
GACTGACCTTCTCGGCGGCGCTCAAGGCCTTCCTGCGCCAGGATCCGGACATCATCATGGTCGGCGAAATCCGCGACCTC
GAGACCGCCGACATCGCGATCAAGGCCGCGCAGACCGGCCACATGGTGTTCTCGACGCTGCACACCAACGATGCCCCCGG
CACGCTGACCCGTCTGCTGAACATGGGCGTCGCGCCGTTCAACGTCGCCAGCTCGGTGATCCTGATCACCGCCCAGCGGC
TGGCACGTAAGCTGTGCACGCAGTGCAAGACGCCGGCCGACATTCCCGACGAAGCGCTGCTGCGCGCCGGCTTCACCGAA
GCCCAGCTCGACGGCAGCTGGCGCCCGTACAAGGCCGTGGGCTGCGATCATTGCAAGGGGACCGGCTACAAGGGCCGGGT
CGGCATTTATCAGGTGATGCCCATTTCGGACGAAATGAACCGCCTGATCATGAAAAACGGCACGTCGATCGACCTCGCCG
ACCAGGCCAGGCGCGAGGGCGTACGCGACCTGCGACAATCCGGACTCTTGAAAGTCAAACAAGGCATGACCTCGCTCGAG
GAAATCATGGCCGTCACCAACGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-146)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1D9BH38

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.867

100

0.562
  pilB Acinetobacter baylyi ADP1

55.166

100

0.555
  pilF Neisseria gonorrhoeae MS11

55.872

98.944

0.553
  pilB Legionella pneumophila strain ERS1305867

53.805

99.472

0.535
  pilB Vibrio cholerae strain A1552

48.135

99.12

0.477
  pilB Vibrio parahaemolyticus RIMD 2210633

46.975

98.944

0.465
  pilB Vibrio campbellii strain DS40M4

46.549

99.472

0.463
  pilF Thermus thermophilus HB27

37.805

100

0.382
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.494

92.606

0.375

Multiple sequence alignment