Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   BJK08_RS07395 Genome accession   NZ_CP017602
Coordinates   1628853..1630073 (+) Length   406 a.a.
NCBI ID   WP_027221710.1    Uniprot ID   A0A2S6EZN5
Organism   Legionella pneumophila strain D5945     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1623853..1635073
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BJK08_RS07375 (BJK08_07260) - 1624946..1625515 (-) 570 WP_027221706.1 hypoxanthine-guanine phosphoribosyltransferase -
  BJK08_RS07380 (BJK08_07265) - 1625558..1625884 (-) 327 WP_027227020.1 DUF1820 family protein -
  BJK08_RS07385 (BJK08_07270) - 1626067..1626810 (-) 744 WP_027227021.1 methyltransferase domain-containing protein -
  BJK08_RS07390 (BJK08_07275) pilB 1627123..1628847 (+) 1725 WP_027227022.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BJK08_RS07395 (BJK08_07280) pilC 1628853..1630073 (+) 1221 WP_027221710.1 type II secretion system F family protein Machinery gene
  BJK08_RS07400 (BJK08_07285) pilD 1630119..1630982 (+) 864 WP_027227023.1 prepilin peptidase Machinery gene
  BJK08_RS07405 (BJK08_07290) - 1630996..1632201 (-) 1206 WP_027227024.1 cyclic nucleotide-binding domain-containing protein -
  BJK08_RS07410 (BJK08_07295) - 1632311..1632904 (-) 594 WP_027227025.1 hypothetical protein -
  BJK08_RS07415 (BJK08_07300) - 1632920..1633375 (-) 456 WP_027227026.1 hypothetical protein -
  BJK08_RS07420 (BJK08_07305) - 1633523..1634338 (+) 816 WP_027227027.1 pyruvate, water dikinase regulatory protein -

Sequence

Protein


Download         Length: 406 a.a.        Molecular weight: 44641.85 Da        Isoelectric Point: 9.6052

>NTDB_id=200677 BJK08_RS07395 WP_027221710.1 1628853..1630073(+) (pilC) [Legionella pneumophila strain D5945]
MDKNAPPLLTFHYQGINKAGQKMEGDIQARSLAIAKADLRKQGIVTNKVVKKRKPLLDRKNKKITQADITVFSRQLATMI
ESGIPLVQAFDIVAKGQSNKRLKDLIETIKHDIETGLTLAESLIKHPLYFNELFCNLVDAGEKSGSLDIMLDKVATYKEK
IEIIKKKIKKALTYPIAVMVVALLVTAGLLIYVVPQFESLFKGFGADLPAMTRAVITMSEFMQAYWYIIFGALGGVVYSF
FYAKNHSLDFAQTIDRVMLKFPVIGPILEKAAIARFARTLSITFAAGLPLVEALKSVAGATGNIIYAKATDKIREEVATG
QQMFIAMENTHLFPNMVIQMVAIGEESGALEKMLSKVADFYEEEVNNAVDALSSLLEPIIMSILGILVGGLVVAMYLPIF
KLGSAV

Nucleotide


Download         Length: 1221 bp        

>NTDB_id=200677 BJK08_RS07395 WP_027221710.1 1628853..1630073(+) (pilC) [Legionella pneumophila strain D5945]
ATGGATAAAAATGCCCCACCTTTATTAACCTTTCATTATCAAGGCATCAATAAAGCTGGTCAAAAAATGGAAGGTGATAT
TCAAGCCAGAAGCTTGGCAATAGCTAAAGCTGATTTGCGTAAACAAGGAATAGTCACTAATAAAGTAGTTAAAAAAAGAA
AACCCTTGTTGGATAGAAAAAATAAAAAAATCACGCAAGCGGATATTACAGTGTTTAGCCGCCAATTAGCTACGATGATA
GAGTCAGGGATACCATTAGTACAAGCGTTTGATATTGTTGCCAAAGGACAAAGTAATAAAAGGCTTAAAGACTTGATAGA
AACAATTAAGCACGATATTGAAACGGGGCTAACGCTAGCAGAATCGTTAATTAAACATCCACTCTATTTTAATGAATTAT
TCTGTAATCTTGTCGATGCAGGTGAAAAATCAGGTTCATTAGATATTATGCTTGACAAGGTTGCAACGTATAAAGAAAAA
ATTGAAATCATAAAAAAGAAAATAAAGAAAGCTTTAACTTACCCAATTGCAGTTATGGTTGTCGCGCTTTTGGTTACTGC
AGGATTGTTAATCTACGTGGTTCCTCAATTTGAATCGCTGTTCAAAGGATTTGGCGCTGATTTGCCAGCGATGACCAGAG
CTGTAATTACTATGTCTGAATTTATGCAGGCCTATTGGTATATTATTTTTGGTGCTTTGGGTGGAGTTGTCTATAGCTTT
TTTTATGCCAAAAATCATTCTTTAGATTTCGCGCAAACCATTGACCGAGTAATGCTAAAGTTTCCTGTGATAGGACCCAT
TTTAGAGAAAGCTGCTATTGCTCGATTTGCGAGGACACTATCTATTACTTTTGCTGCTGGATTACCTTTAGTTGAAGCTT
TGAAATCTGTAGCTGGAGCGACTGGAAATATTATTTATGCCAAGGCAACTGATAAAATAAGAGAGGAAGTTGCCACAGGG
CAGCAAATGTTTATTGCTATGGAAAATACCCATTTATTCCCAAATATGGTAATTCAAATGGTAGCAATTGGTGAGGAATC
AGGTGCCCTTGAGAAAATGTTAAGCAAGGTTGCTGATTTTTATGAGGAAGAAGTAAATAATGCTGTAGATGCTCTGAGTA
GTTTATTGGAGCCTATAATTATGTCAATATTAGGCATCCTTGTTGGGGGATTGGTCGTGGCTATGTATTTGCCTATCTTC
AAACTGGGCTCTGCTGTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-398)

Type II secretion system (T2SS), protein F (T2SSF)

(72-195)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2S6EZN5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Legionella pneumophila strain ERS1305867

99.015

100

0.99
  pilC Acinetobacter baumannii D1279779

54.074

99.754

0.539
  pilC Pseudomonas stutzeri DSM 10701

54.293

97.537

0.53
  pilC Acinetobacter baylyi ADP1

53.401

97.783

0.522
  pilG Neisseria meningitidis 44/76-A

45.088

97.783

0.441
  pilG Neisseria gonorrhoeae MS11

44.836

97.783

0.438
  pilC Vibrio cholerae strain A1552

42.5

98.522

0.419
  pilC Vibrio campbellii strain DS40M4

39.409

100

0.394

Multiple sequence alignment