Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BI364_RS14440 Genome accession   NZ_CP017415
Coordinates   2986779..2988506 (+) Length   575 a.a.
NCBI ID   WP_070079345.1    Uniprot ID   -
Organism   Acidihalobacter yilgarnensis strain F5     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2981779..2993506
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BI364_RS14420 (BI364_14420) - 2982415..2983155 (+) 741 WP_070079341.1 bifunctional 2-polyprenyl-6-hydroxyphenol methylase/3-demethylubiquinol 3-O-methyltransferase UbiG -
  BI364_RS14425 (BI364_14425) - 2983152..2984186 (+) 1035 WP_083251432.1 glycosyltransferase family 4 protein -
  BI364_RS14430 (BI364_14430) - 2984175..2985263 (-) 1089 WP_070079343.1 glycosyltransferase family 1 protein -
  BI364_RS14435 (BI364_14435) - 2985331..2986638 (-) 1308 WP_070079344.1 oligosaccharide flippase family protein -
  BI364_RS14440 (BI364_14440) pilB 2986779..2988506 (+) 1728 WP_070079345.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BI364_RS14445 (BI364_14445) pilC 2988543..2989763 (+) 1221 WP_070079346.1 type II secretion system F family protein Machinery gene
  BI364_RS14450 (BI364_14450) pilD 2989832..2990722 (+) 891 WP_070079347.1 A24 family peptidase Machinery gene
  BI364_RS14455 (BI364_14455) coaE 2990719..2991330 (+) 612 WP_070079348.1 dephospho-CoA kinase -
  BI364_RS14460 (BI364_14460) - 2991344..2991559 (-) 216 WP_156782781.1 hypothetical protein -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 63567.07 Da        Isoelectric Point: 5.7808

>NTDB_id=197997 BI364_RS14440 WP_070079345.1 2986779..2988506(+) (pilB) [Acidihalobacter yilgarnensis strain F5]
MSTPATQLRLSGLARHLVRSGLLKEEEAYGLQEQSQQKRIPLVSLLIKQSHLTPPQVALAAAEEFGIPLFDLSVFDLEYL
PKALINEKLVRQNRALPLFQRGNRLFVAISDPMNLGSVDELKFNTGIQTEPLMVEDDKLDRSIEEVLSAMDSSMSELMGD
SDLEGLDRLEVAEAPEDNNAASTPDQDDAPVVRYVNKVLLDAIKRGASDIHFEPYEKYFRLRLRIDGMLHEVARPPVAMA
ARISSRIKVMSRMDVVERRKPQDGRIKLQISKNRAIDFRVNSLPTLFGEKIVLRILDPTSAQLGVEALGFEEEQRRLYLS
ALKKPYGMILVTGPTGSGKTVTLYTGLGILNTEDRNISTAEDPAEINMPGINQVNVNPKVGLTFAEALRAFLRQDPDIVM
VGEIRDLETAEISIKAAQTGHLVLSTLHTNDAPQTLTRLMNMGVPAYNIANSVSLIIAQRLARRLCPHCKQVHDVPNEAL
LDEGFDTADVGTFKLYKPNIQGCNKCSGGYKGRLGIYQVMPVSDEMGRMIMAGSNAMDIAAQAKKEGVDDLRASGLRKVK
DGVTSLDELNRVTKD

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=197997 BI364_RS14440 WP_070079345.1 2986779..2988506(+) (pilB) [Acidihalobacter yilgarnensis strain F5]
ATGTCAACACCTGCGACGCAGCTGAGGCTTAGTGGCCTCGCGCGTCATCTGGTTCGTAGTGGCCTGCTCAAGGAGGAGGA
GGCCTACGGGCTGCAAGAACAGTCGCAGCAAAAGCGCATTCCGCTGGTCAGCCTGCTCATCAAGCAGAGTCATCTGACCC
CTCCTCAGGTTGCGCTCGCTGCTGCCGAAGAATTCGGCATCCCCCTGTTCGACCTGTCGGTGTTCGACCTCGAATATCTA
CCCAAAGCGCTCATCAACGAAAAGTTGGTTCGACAAAACCGGGCCCTGCCGCTGTTTCAGCGTGGGAACCGACTCTTCGT
CGCCATTTCAGACCCCATGAATCTTGGCTCGGTCGATGAGCTGAAGTTCAATACCGGCATCCAGACCGAACCGCTCATGG
TCGAGGACGACAAACTCGACCGCTCAATCGAGGAAGTCCTGAGCGCCATGGATTCGTCCATGAGCGAATTGATGGGCGAC
TCGGACCTCGAAGGACTCGATCGACTGGAAGTCGCAGAGGCGCCGGAGGATAACAACGCCGCCTCCACACCGGATCAGGA
TGACGCGCCCGTGGTGCGCTACGTCAACAAGGTGCTGCTCGATGCCATCAAGCGCGGCGCCTCGGACATCCACTTCGAAC
CCTACGAAAAATACTTCCGCCTGCGTCTACGCATAGACGGCATGCTCCACGAAGTTGCCCGCCCACCGGTCGCCATGGCG
GCCCGGATATCCTCCCGCATCAAGGTCATGTCGCGTATGGATGTGGTAGAGCGACGCAAACCGCAGGACGGGCGCATCAA
GCTCCAAATCAGCAAGAATCGTGCGATCGATTTCCGTGTAAACAGCTTGCCAACATTGTTCGGCGAGAAGATCGTACTGC
GTATACTCGACCCAACCAGCGCCCAGCTCGGTGTTGAAGCCCTCGGCTTCGAGGAGGAGCAACGCAGACTTTACCTGAGC
GCGCTGAAAAAACCATACGGCATGATTTTGGTCACCGGCCCCACCGGCAGCGGCAAGACCGTGACACTCTACACTGGCCT
CGGCATCCTCAATACCGAGGATCGAAATATCTCGACTGCCGAGGACCCGGCCGAAATCAACATGCCTGGCATCAACCAGG
TCAACGTCAATCCCAAGGTCGGCTTGACCTTCGCCGAGGCTCTGCGAGCCTTTTTGCGCCAGGATCCCGACATCGTAATG
GTCGGTGAGATACGCGATCTCGAAACGGCGGAGATCTCGATCAAGGCCGCACAGACGGGGCATCTGGTGCTCTCGACGCT
GCATACCAACGATGCACCGCAGACGCTGACCCGCCTCATGAACATGGGCGTACCGGCGTACAACATCGCCAATTCGGTTT
CGCTCATCATCGCCCAACGCCTAGCAAGACGGCTCTGCCCCCACTGCAAGCAGGTACACGACGTGCCGAATGAAGCCTTG
CTTGATGAAGGCTTTGATACCGCCGACGTCGGCACCTTCAAGCTCTACAAGCCCAACATACAGGGCTGCAACAAGTGCAG
CGGGGGTTACAAGGGTCGCTTGGGCATTTACCAAGTCATGCCCGTATCCGACGAAATGGGACGCATGATCATGGCGGGTA
GCAATGCCATGGATATCGCCGCGCAGGCCAAAAAAGAAGGGGTCGACGATCTCCGCGCCTCCGGCCTCAGAAAAGTGAAA
GACGGCGTGACCAGCCTGGATGAACTCAACCGCGTCACCAAGGACTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-151)

Type II/IV secretion system protein (T2SSE)

(197-464)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55

100

0.555
  pilB Acinetobacter baylyi ADP1

54.844

100

0.551
  pilB Legionella pneumophila strain ERS1305867

54.231

100

0.546
  pilF Neisseria gonorrhoeae MS11

49.12

98.783

0.485
  pilB Vibrio parahaemolyticus RIMD 2210633

48.404

98.087

0.475
  pilB Vibrio cholerae strain A1552

52.427

89.565

0.47
  pilB Vibrio campbellii strain DS40M4

47.266

98.609

0.466
  pilF Thermus thermophilus HB27

37.39

98.609

0.369
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.098

92.522

0.362

Multiple sequence alignment