Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   BI364_RS14450 Genome accession   NZ_CP017415
Coordinates   2989832..2990722 (+) Length   296 a.a.
NCBI ID   WP_070079347.1    Uniprot ID   A0A1D8IRI8
Organism   Acidihalobacter yilgarnensis strain F5     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2984832..2995722
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BI364_RS14435 (BI364_14435) - 2985331..2986638 (-) 1308 WP_070079344.1 oligosaccharide flippase family protein -
  BI364_RS14440 (BI364_14440) pilB 2986779..2988506 (+) 1728 WP_070079345.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BI364_RS14445 (BI364_14445) pilC 2988543..2989763 (+) 1221 WP_070079346.1 type II secretion system F family protein Machinery gene
  BI364_RS14450 (BI364_14450) pilD 2989832..2990722 (+) 891 WP_070079347.1 A24 family peptidase Machinery gene
  BI364_RS14455 (BI364_14455) coaE 2990719..2991330 (+) 612 WP_070079348.1 dephospho-CoA kinase -
  BI364_RS14460 (BI364_14460) - 2991344..2991559 (-) 216 WP_156782781.1 hypothetical protein -
  BI364_RS14465 (BI364_14465) - 2991684..2995496 (+) 3813 WP_070079350.1 AAA family ATPase -

Sequence

Protein


Download         Length: 296 a.a.        Molecular weight: 32346.38 Da        Isoelectric Point: 7.2574

>NTDB_id=197999 BI364_RS14450 WP_070079347.1 2989832..2990722(+) (pilD) [Acidihalobacter yilgarnensis strain F5]
MTLPIPIPYLVLLILTGLVIGSFVNVVIHRLPRMMALQWRRECAELGASEAHTPTEDDDAHPQKNETYNLVVPRSSCPHC
GHMIAATENIPILSYLWQRGRCAACRAPISPRYPIIELLGALLAAMAGLRFGLSWETLAAAVLGWSLLAAAAIDLETQLL
PDSITLPLLWLGLLLNIDGLFVPLRDAVIGAMAGYLSLWLIYHLFRLLTGKEGMGYGDFKLLALLGAWFGWQLLPLTILL
SSLVGALAGLSLMAFRRHHHEIPIPFGPYLAAAGWIALLWGHALTGAYLRFAHIGG

Nucleotide


Download         Length: 891 bp        

>NTDB_id=197999 BI364_RS14450 WP_070079347.1 2989832..2990722(+) (pilD) [Acidihalobacter yilgarnensis strain F5]
ATGACCCTGCCGATACCCATCCCCTACCTCGTTCTGTTAATCCTAACCGGCTTGGTGATCGGAAGCTTCGTGAACGTGGT
CATTCACCGCCTTCCACGTATGATGGCGCTACAGTGGCGGCGCGAATGCGCCGAGCTGGGCGCCTCCGAAGCGCACACGC
CCACAGAGGACGACGACGCGCATCCTCAAAAAAACGAAACTTACAACTTGGTTGTGCCTCGCTCATCTTGCCCGCATTGC
GGGCACATGATTGCAGCGACCGAGAACATCCCGATCCTCAGTTATCTTTGGCAACGCGGGCGCTGCGCTGCCTGCCGTGC
CCCGATTTCGCCACGCTACCCGATCATAGAGCTACTGGGCGCCCTGCTTGCCGCAATGGCAGGATTGCGTTTCGGCCTCA
GCTGGGAAACGCTGGCGGCGGCCGTGCTCGGCTGGAGTCTGCTCGCCGCTGCTGCCATCGATCTCGAAACTCAGCTACTG
CCCGACAGCATCACCCTACCGCTGCTATGGCTCGGCTTGCTGCTGAATATCGACGGGCTATTCGTACCGTTGCGCGACGC
CGTCATCGGGGCGATGGCGGGTTATTTGAGCCTGTGGCTGATCTATCACCTGTTCCGCCTACTGACCGGTAAAGAGGGCA
TGGGCTACGGCGATTTCAAACTGCTCGCCTTGCTCGGCGCGTGGTTCGGCTGGCAGCTACTGCCACTGACCATACTGTTG
TCCTCCTTGGTTGGCGCCCTCGCGGGGCTGTCCCTGATGGCCTTTCGGCGTCACCACCACGAGATTCCCATACCCTTCGG
CCCCTATCTCGCGGCGGCTGGTTGGATCGCCCTGCTGTGGGGACACGCGCTGACCGGCGCGTATCTGCGCTTCGCGCATA
TTGGCGGATGA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(142-250)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(15-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1D8IRI8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio campbellii strain DS40M4

51.93

96.284

0.5
  pilD Vibrio cholerae strain A1552

52.5

94.595

0.497
  pilD Acinetobacter nosocomialis M2

48

92.905

0.446
  pilD Acinetobacter baumannii D1279779

47.636

92.905

0.443
  pilD Neisseria gonorrhoeae MS11

47.253

92.23

0.436

Multiple sequence alignment