Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   Q5W_RS02450 Genome accession   NZ_CP017311
Coordinates   488183..489913 (-) Length   576 a.a.
NCBI ID   WP_035620178.1    Uniprot ID   -
Organism   Hydrogenophaga sp. PBC     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 483183..494913
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q5W_RS02420 (Q5W_02420) - 484005..484454 (+) 450 WP_009516500.1 NUDIX domain-containing protein -
  Q5W_RS02425 (Q5W_02425) - 484468..484665 (-) 198 WP_009516501.1 DNA gyrase inhibitor YacG -
  Q5W_RS02430 (Q5W_02430) zapD 484670..485425 (-) 756 WP_009516502.1 cell division protein ZapD -
  Q5W_RS25590 (Q5W_02435) coaE 485469..486077 (-) 609 WP_009516503.1 dephospho-CoA kinase -
  Q5W_RS02440 (Q5W_02440) pilD 486074..486931 (-) 858 WP_009516504.1 A24 family peptidase Machinery gene
  Q5W_RS02445 (Q5W_02445) pilC 486931..488148 (-) 1218 WP_009516505.1 type II secretion system F family protein Machinery gene
  Q5W_RS02450 (Q5W_02450) pilB 488183..489913 (-) 1731 WP_035620178.1 type IV-A pilus assembly ATPase PilB Machinery gene
  Q5W_RS02460 (Q5W_02460) - 490194..491123 (-) 930 WP_138820106.1 polyprenyl synthetase family protein -
  Q5W_RS02465 (Q5W_02465) rplU 491398..491709 (+) 312 WP_009516508.1 50S ribosomal protein L21 -
  Q5W_RS02470 (Q5W_02470) rpmA 491721..491978 (+) 258 WP_009516509.1 50S ribosomal protein L27 -
  Q5W_RS02475 (Q5W_02475) obgE 492052..493122 (+) 1071 WP_009516510.1 GTPase ObgE -
  Q5W_RS02480 (Q5W_02480) proB 493141..494298 (+) 1158 WP_009516511.1 glutamate 5-kinase -
  Q5W_RS02485 (Q5W_02485) - 494305..494817 (-) 513 WP_009516512.1 CNP1-like family protein -

Sequence

Protein


Download         Length: 576 a.a.        Molecular weight: 62856.39 Da        Isoelectric Point: 5.8011

>NTDB_id=197569 Q5W_RS02450 WP_035620178.1 488183..489913(-) (pilB) [Hydrogenophaga sp. PBC]
MASVDTVNQDSPSMALPGLGRALVSAGKLNQRAAEDLYRKAQSSRTSFIAELTGSGAVSPSDLAHTMSVAFAAPLLDLDA
IDAQRLPAGLLDAKICADFRIVVLSKRNNRLIVATADPSDQQAAEKIKFATQMGVDWVIAEFDKLSRLVEAQTKSVTEAM
ENIVGDVEFDELATEAVSADAAEKASEVDDAPVVKFLHKMLIDAFNMRASDLHFEPYEHQYRVRFRIDGELREIASPPIV
IKDKLASRIKVISRMDISEKRVPQDGRMKLKVGPDRVIDFRVSTLPTLFGEKIVIRILDPSQAKMGIDALGYEPEEKERL
MDAIQRPYGMVLVTGPTGSGKTVSLYTCLNILNKPGVNISTAEDPSEINLPGVNQVNMNEKAGLTFAVALKAFLRQDPDV
IMVGEIRDLETADIAIKAAQTGHMVMSTLHTNDAPTTLTRMMNMGIPTFNIASSVILITAQRLARRLCPNCKAPLDVPRK
ALLEAGYKPEELDGTWTPYKPVGCSACNNGYKGRVGIYQVMPISEEIQRIILRGGTSLDIAQQASKEGVRSLRESGLLKV
KLGHTSLEEVLSVTNE

Nucleotide


Download         Length: 1731 bp        

>NTDB_id=197569 Q5W_RS02450 WP_035620178.1 488183..489913(-) (pilB) [Hydrogenophaga sp. PBC]
ATGGCGTCAGTTGATACGGTGAACCAGGATAGCCCGTCCATGGCCCTTCCGGGGCTGGGGCGTGCGCTCGTGTCGGCCGG
CAAGCTCAATCAACGGGCGGCCGAGGATCTGTACCGCAAGGCACAAAGCAGTCGAACCAGTTTCATCGCCGAGTTGACGG
GCTCAGGTGCCGTGTCTCCGAGCGATCTGGCCCACACCATGTCGGTGGCCTTCGCCGCGCCGCTGCTCGATCTCGATGCG
ATTGATGCACAGCGCCTGCCCGCCGGCTTGCTCGATGCCAAGATCTGTGCGGACTTTCGCATCGTCGTCCTCAGCAAGCG
CAACAACCGCTTGATCGTCGCCACGGCCGATCCATCCGATCAGCAGGCCGCTGAAAAGATCAAATTCGCCACGCAAATGG
GCGTGGACTGGGTCATCGCCGAATTCGACAAGCTCAGTCGCTTGGTCGAGGCGCAGACCAAGTCCGTCACCGAGGCGATG
GAAAACATCGTCGGCGATGTCGAATTCGACGAGTTGGCCACCGAAGCCGTCAGCGCAGACGCCGCCGAGAAGGCCAGCGA
GGTCGACGATGCGCCGGTCGTCAAGTTCCTGCACAAGATGCTGATCGACGCGTTCAACATGCGCGCGTCCGACCTGCACT
TCGAGCCCTACGAGCACCAGTACCGCGTGCGTTTCCGCATCGACGGCGAACTGCGTGAAATCGCTTCGCCGCCGATCGTT
ATCAAGGACAAGCTGGCGTCGCGGATCAAGGTCATATCCCGGATGGACATCTCCGAGAAGCGGGTCCCCCAGGACGGGCG
GATGAAGCTCAAGGTGGGCCCGGACCGCGTGATCGACTTCCGCGTCAGCACCCTGCCCACGCTGTTCGGTGAAAAGATCG
TGATCCGTATCCTCGATCCGAGCCAGGCCAAGATGGGCATCGACGCCCTGGGCTACGAGCCGGAAGAGAAGGAGCGCCTG
ATGGACGCCATCCAGCGTCCCTATGGCATGGTGCTCGTCACTGGCCCGACGGGCTCAGGCAAGACGGTGTCGCTGTACAC
CTGCCTGAACATCCTGAACAAGCCGGGTGTGAACATCTCGACGGCCGAAGACCCGTCCGAGATCAACTTGCCCGGCGTCA
ACCAGGTGAACATGAACGAGAAGGCCGGCCTGACCTTCGCGGTCGCGCTCAAGGCCTTCCTGCGCCAGGATCCGGACGTG
ATCATGGTCGGTGAGATCCGAGACCTGGAAACCGCCGACATCGCAATCAAGGCCGCGCAGACCGGCCACATGGTGATGTC
GACCCTGCACACCAACGACGCGCCGACGACGCTCACGCGGATGATGAACATGGGCATCCCCACGTTCAACATCGCCTCCA
GCGTGATCCTCATCACCGCGCAACGGCTCGCGCGGCGCCTGTGCCCGAATTGCAAGGCGCCGCTGGACGTGCCCCGCAAG
GCCCTGCTCGAGGCGGGTTACAAGCCCGAGGAACTCGATGGCACCTGGACGCCCTACAAGCCGGTCGGCTGCTCAGCCTG
CAACAACGGCTACAAGGGGCGTGTCGGCATCTATCAGGTGATGCCGATCTCCGAGGAGATCCAACGCATCATTTTGCGCG
GCGGCACCTCGCTGGACATCGCCCAGCAAGCCAGCAAGGAAGGCGTTCGCTCGCTGCGCGAGTCCGGCCTGCTCAAAGTC
AAACTGGGGCACACCTCCCTTGAGGAAGTCCTCAGCGTGACGAACGAATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(53-149)

Type II/IV secretion system protein (T2SSE)

(199-466)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.626

97.569

0.533
  pilB Acinetobacter baylyi ADP1

54.352

97.743

0.531
  pilF Neisseria gonorrhoeae MS11

52.847

97.569

0.516
  pilB Legionella pneumophila strain ERS1305867

50.088

98.09

0.491
  pilB Vibrio parahaemolyticus RIMD 2210633

45.161

96.875

0.437
  pilB Vibrio campbellii strain DS40M4

44.742

97.396

0.436
  pilB Vibrio cholerae strain A1552

47.195

89.757

0.424
  pilF Thermus thermophilus HB27

37.478

97.743

0.366

Multiple sequence alignment