Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   BFO62_RS10830 Genome accession   NZ_CP017257
Coordinates   1883271..1884947 (-) Length   558 a.a.
NCBI ID   WP_076612603.1    Uniprot ID   -
Organism   Neisseria meningitidis strain COL-201504-11     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1878271..1889947
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BFO62_RS10810 (BFO62_09765) pilG 1880183..1881415 (+) 1233 WP_076612607.1 type 4 pilus assembly protein PilG Machinery gene
  BFO62_RS10815 (BFO62_09770) pilD 1881490..1882350 (+) 861 WP_076612606.1 prepilin peptidase Machinery gene
  BFO62_RS10820 (BFO62_09775) coaE 1882352..1882984 (+) 633 WP_076612605.1 dephospho-CoA kinase -
  BFO62_RS10825 (BFO62_09780) - 1882977..1883186 (+) 210 WP_076612604.1 DNA gyrase inhibitor YacG -
  BFO62_RS10830 (BFO62_09785) pilF 1883271..1884947 (-) 1677 WP_076612603.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BFO62_RS10840 (BFO62_09795) - 1885439..1885885 (-) 447 WP_002220271.1 DUF441 domain-containing protein -
  BFO62_RS10845 (BFO62_09800) ispB 1885890..1886864 (-) 975 WP_002220270.1 octaprenyl diphosphate synthase -
  BFO62_RS10855 (BFO62_09805) rplU 1887088..1887396 (+) 309 WP_002216394.1 50S ribosomal protein L21 -
  BFO62_RS10860 (BFO62_09810) rpmA 1887421..1887693 (+) 273 WP_002212328.1 50S ribosomal protein L27 -
  BFO62_RS10865 (BFO62_09815) ubiM 1887928..1889112 (+) 1185 WP_002220268.1 5-demethoxyubiquinol-8 5-hydroxylase UbiM -
  BFO62_RS10870 (BFO62_09820) rpmG 1889383..1889538 (-) 156 WP_002212306.1 50S ribosomal protein L33 -
  BFO62_RS10875 (BFO62_09825) rpmB 1889570..1889803 (-) 234 WP_002216391.1 50S ribosomal protein L28 -

Sequence

Protein


Download         Length: 558 a.a.        Molecular weight: 61964.06 Da        Isoelectric Point: 5.4143

>NTDB_id=197017 BFO62_RS10830 WP_076612603.1 1883271..1884947(-) (pilF) [Neisseria meningitidis strain COL-201504-11]
MSVGLLRILVQNQVVTVEQAEHYYNESQAGKEVLPMLFSDGVISPKSLAALIARVFSYSILDLRHYPRHRVLMGVLTEEQ
MVEFHCVPVFRRGNKVFFAVSDPTQMPQIQKTVSAAGIEVELVIVEDDQLAGLLDWVGSRSTSLLQELGEGQEEEESHTL
YIDNEEAEDGPVPRFIHKTLSDALRSGASDIHFEFYEHNARIRFRVDGQLREVVQPPIAVRGQLASRIKVMSRLDISEKR
IPQDGRMQLTFQKGGKPVDFRVSTLPTLFGEKVVMRILNSDAASLNIDQLGFEPFQKKLLLEAIHRPYGMVLVTGPTGSG
KTVSLYTCLNILNTESVNIATAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETADIAIKAAQ
TGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSSCKQEVERPSASALKEVGFTDEDLAKDWKLY
RAVGCDRCRGQGYKGRAGVYEVMPISEEMQRVIMNNGTEVDILDVAYKEGMVDLRRAGILKVMQGITSLEEVTANTND

Nucleotide


Download         Length: 1677 bp        

>NTDB_id=197017 BFO62_RS10830 WP_076612603.1 1883271..1884947(-) (pilF) [Neisseria meningitidis strain COL-201504-11]
ATGAGCGTAGGTTTGCTGAGGATTCTGGTTCAAAACCAGGTGGTTACTGTTGAGCAGGCCGAGCATTACTACAATGAGTC
GCAGGCGGGTAAGGAAGTGTTGCCGATGCTGTTTTCAGACGGTGTCATTTCGCCCAAGTCGCTTGCGGCATTGATTGCGA
GGGTGTTCAGTTATTCGATTCTTGATTTGCGTCATTATCCGCGCCACAGGGTGCTGATGGGGGTGTTGACGGAGGAGCAG
ATGGTGGAGTTCCACTGTGTGCCGGTTTTCCGTCGGGGCAACAAAGTATTTTTTGCGGTTTCCGATCCGACACAGATGCC
GCAAATTCAGAAAACCGTTTCTGCCGCAGGGATTGAGGTTGAGTTGGTCATTGTCGAGGATGACCAGTTGGCGGGTTTGC
TCGATTGGGTGGGTTCGCGTTCGACATCGCTGCTTCAGGAGCTTGGGGAGGGGCAGGAGGAAGAGGAAAGCCACACTCTG
TATATCGACAATGAGGAGGCTGAGGACGGTCCTGTTCCGAGGTTTATCCATAAAACTTTGTCTGATGCCTTGCGCAGCGG
GGCATCAGACATCCATTTTGAGTTTTACGAACACAATGCCCGTATCCGTTTCCGTGTGGACGGGCAGCTCCGCGAGGTGG
TTCAGCCGCCCATTGCGGTAAGGGGGCAGCTTGCTTCCCGGATTAAGGTAATGTCGCGTTTGGACATTTCCGAAAAACGG
ATACCGCAGGACGGCAGGATGCAGCTGACCTTTCAAAAGGGCGGCAAGCCTGTCGATTTCCGTGTCAGCACATTGCCGAC
GCTGTTTGGCGAAAAGGTTGTGATGCGGATTTTGAATTCCGATGCCGCGTCTTTGAACATCGACCAGCTCGGTTTTGAGC
CGTTTCAGAAAAAATTGTTGTTGGAGGCGATTCACCGTCCTTACGGGATGGTGCTGGTAACCGGCCCGACGGGTTCGGGT
AAGACGGTGTCGCTCTATACCTGTTTGAATATTTTGAATACGGAGTCGGTAAATATTGCAACGGCGGAAGACCCTGCCGA
GATTAACCTGCCGGGCATCAATCAGGTTAACGTCAATGATAAGCAGGGTCTGACTTTTGCCGCCGCTTTGAAGTCTTTCC
TGCGTCAGGATCCGGACATCATTATGGTCGGTGAGATTCGTGATTTGGAAACTGCCGATATTGCGATTAAGGCGGCGCAG
ACCGGTCATATGGTGTTTTCCACCCTGCACACCAATAATGCTCCGGCGACGTTGTCGCGTATGCTGAATATGGGTGTCGC
GCCGTTTAATATTGCCAGTTCGGTCAGCCTGATTATGGCGCAGCGTCTTTTACGCAGGCTGTGTTCGAGCTGCAAACAGG
AAGTGGAACGTCCGTCTGCCTCTGCTTTGAAGGAAGTCGGCTTCACCGATGAGGACCTTGCAAAAGATTGGAAACTTTAC
CGCGCCGTCGGTTGCGACCGTTGCCGGGGGCAGGGTTATAAAGGGCGTGCGGGCGTGTATGAGGTTATGCCCATCAGCGA
AGAAATGCAGCGTGTGATTATGAACAACGGTACGGAAGTGGATATTTTGGACGTTGCCTATAAGGAGGGTATGGTGGATT
TGCGCCGGGCCGGTATTTTGAAAGTTATGCAGGGCATTACTTCATTGGAAGAGGTAACGGCAAATACCAACGATTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-139)

Type II/IV secretion system protein (T2SSE)

(178-446)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

99.283

100

0.993
  pilB Acinetobacter baumannii D1279779

50.265

100

0.511
  pilB Acinetobacter baylyi ADP1

50.356

100

0.507
  pilB Legionella pneumophila strain ERS1305867

47.527

100

0.482
  pilB Vibrio cholerae strain A1552

45.552

100

0.459
  pilB Vibrio parahaemolyticus RIMD 2210633

45.39

100

0.459
  pilB Vibrio campbellii strain DS40M4

43.838

100

0.446
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.153

93.728

0.376
  pilF Thermus thermophilus HB27

37.132

97.491

0.362

Multiple sequence alignment