Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BFX80_RS13760 Genome accession   NZ_CP017114
Coordinates   3266470..3268206 (+) Length   578 a.a.
NCBI ID   WP_084209203.1    Uniprot ID   -
Organism   Cobetia marina strain JCM 21022     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3261470..3273206
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BFX80_RS13740 (BFX80_13405) - 3261874..3263433 (+) 1560 WP_084209200.1 ABC transporter permease -
  BFX80_RS13745 (BFX80_13410) - 3263512..3264792 (+) 1281 WP_084209201.1 cation diffusion facilitator family transporter -
  BFX80_RS13750 (BFX80_13415) - 3264917..3265219 (+) 303 WP_077371726.1 hypothetical protein -
  BFX80_RS13755 pilA 3265266..3265706 (-) 441 WP_084209202.1 pilin Machinery gene
  BFX80_RS13760 (BFX80_13420) pilB 3266470..3268206 (+) 1737 WP_084209203.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BFX80_RS13765 (BFX80_13425) pilC 3268452..3269690 (+) 1239 WP_084209204.1 type II secretion system F family protein Machinery gene
  BFX80_RS13770 (BFX80_13430) pilD 3269789..3270649 (+) 861 WP_084209205.1 A24 family peptidase Machinery gene
  BFX80_RS13775 (BFX80_13435) coaE 3270649..3271296 (+) 648 WP_084209206.1 dephospho-CoA kinase -
  BFX80_RS13780 (BFX80_13440) yacG 3271289..3271534 (+) 246 WP_084209207.1 DNA gyrase inhibitor YacG -
  BFX80_RS13785 (BFX80_13445) - 3271663..3272616 (-) 954 WP_077371708.1 Nudix family hydrolase -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 63471.51 Da        Isoelectric Point: 4.8437

>NTDB_id=195762 BFX80_RS13760 WP_084209203.1 3266470..3268206(+) (pilB) [Cobetia marina strain JCM 21022]
MPQSDQTLKGFAARLVESGLLEHQAALLAEQEANEAGESLLLHVITHGLASAREATLVAAWEYGLPYVDLDAIRIDSLPS
IDELPEKVLRKLGVLPLYRREHRLTVGVPSPSTLTHLDELQFATGLSIESVLVAADQLTPALEQYLEAGEESAMDMLGGE
GEALDGLEFEGEEDNNNDLAITANSDDAPVVRFVHKVLLDAIKRGASDIHFEPYESTYRIRFRIDGMLIENTRPPFNMRN
RISARLKVMSRMDISERRLPQDGAIKLRLSKSRSIDFRVNTLPTVYGEKLVLRILDPSSAKMGIEALGFEPEQRAMFEKT
LGEPQGMILVTGPTGSGKTVTLYTGLNILNTPERNISTAEDPVELKVEGVNQVNVLPKIGLDFASALRAFLRQDPDVVMV
GEIRDKETAEIAVKAAQTGHLVLSTLHTNSAPETLTRLSNMGIAPFNIASSVSLIIAQRLARRLCPHCRQPAEIPQEAMA
SMGFSAENGFSAEDLENATIYRPTGCSQCTLGYKGRVGIYEVMPISDEMSRLIMREGTAIELADQARKEGLSDLRRSGLR
KVMAGMTSLEEVNRITKD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=195762 BFX80_RS13760 WP_084209203.1 3266470..3268206(+) (pilB) [Cobetia marina strain JCM 21022]
ATGCCCCAGAGTGATCAGACGTTGAAGGGATTTGCCGCTCGCCTGGTGGAATCAGGCTTGCTGGAGCATCAGGCTGCCTT
GCTGGCTGAGCAGGAGGCCAATGAGGCTGGCGAAAGTCTGCTGCTGCATGTCATCACTCATGGTCTGGCCTCGGCACGTG
AGGCCACCTTGGTGGCAGCATGGGAATACGGGCTGCCCTATGTGGATCTCGATGCAATACGTATCGATTCCCTGCCGAGC
ATTGATGAGCTGCCAGAGAAGGTACTGCGCAAGCTGGGCGTACTGCCACTCTATCGACGTGAACATCGTTTGACCGTAGG
TGTACCCTCGCCATCGACACTGACTCATCTTGATGAACTGCAATTTGCCACCGGCCTGTCGATTGAAAGCGTGCTGGTCG
CAGCTGATCAGCTGACGCCGGCATTGGAGCAGTACCTGGAAGCCGGTGAAGAAAGCGCCATGGATATGCTTGGCGGTGAA
GGTGAAGCCCTCGATGGCTTGGAGTTCGAGGGCGAGGAGGATAACAATAACGACCTTGCCATCACGGCCAACAGTGACGA
TGCGCCGGTGGTGCGCTTCGTTCACAAGGTACTGCTGGATGCCATCAAACGTGGCGCCTCGGATATCCACTTCGAACCCT
ACGAATCCACCTATCGCATTCGTTTTCGTATCGATGGCATGCTGATCGAGAATACTCGCCCGCCCTTCAACATGCGCAAC
CGTATCTCGGCGCGCTTGAAGGTGATGTCGCGCATGGATATCTCCGAGCGGCGTCTGCCTCAGGATGGCGCCATCAAACT
GCGACTTTCCAAGTCACGCTCCATCGATTTTCGTGTCAACACCTTGCCAACCGTTTACGGGGAGAAGTTGGTACTGCGTA
TCTTGGACCCCAGTTCCGCCAAGATGGGCATCGAGGCACTGGGCTTCGAGCCCGAACAGCGTGCCATGTTCGAGAAGACA
CTTGGCGAGCCTCAGGGCATGATCCTTGTCACCGGCCCTACCGGTAGCGGCAAGACCGTCACCCTGTATACCGGCTTGAA
CATCCTCAATACGCCTGAGCGCAATATCTCGACCGCGGAAGACCCGGTCGAGCTCAAGGTAGAGGGTGTCAATCAGGTCA
ACGTTTTGCCCAAGATAGGGCTCGACTTCGCCTCGGCACTGCGCGCCTTCCTGCGCCAGGATCCCGACGTGGTGATGGTC
GGGGAGATTCGGGACAAGGAAACCGCCGAAATTGCCGTCAAGGCCGCGCAGACAGGCCACCTGGTGCTCTCGACCCTGCA
TACCAATTCTGCCCCTGAAACGTTGACGCGCCTCAGCAACATGGGCATCGCGCCCTTCAACATCGCAAGCTCGGTTTCCT
TGATCATCGCGCAACGTCTGGCGCGGCGTCTGTGTCCCCACTGTCGTCAACCTGCCGAGATCCCCCAGGAAGCCATGGCC
TCAATGGGCTTCAGCGCAGAAAATGGCTTCAGTGCGGAAGATCTGGAAAATGCCACCATCTATCGTCCAACGGGTTGCAG
CCAATGCACGCTAGGGTACAAGGGACGCGTCGGCATCTATGAAGTGATGCCCATCTCTGATGAGATGAGCCGCTTGATCA
TGCGGGAAGGCACCGCGATAGAACTGGCAGACCAGGCGCGCAAGGAAGGGTTGTCGGATCTGCGCCGCAGCGGCCTGCGC
AAGGTCATGGCGGGCATGACGTCGCTGGAAGAAGTCAACCGTATCACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(15-150)

Type II/IV secretion system protein (T2SSE)

(196-463)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

52.448

98.962

0.519
  pilB Legionella pneumophila strain ERS1305867

51.839

98.789

0.512
  pilB Acinetobacter baylyi ADP1

51.748

98.962

0.512
  pilB Vibrio campbellii strain DS40M4

47.917

99.654

0.478
  pilF Neisseria gonorrhoeae MS11

48.381

96.194

0.465
  pilB Vibrio cholerae strain A1552

48.115

96.367

0.464
  pilB Vibrio parahaemolyticus RIMD 2210633

48.364

95.156

0.46
  pilB/pilB1 Synechocystis sp. PCC 6803

41.874

90.484

0.379
  pilF Thermus thermophilus HB27

37.876

97.751

0.37
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.698

89.273

0.363

Multiple sequence alignment