Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   BG483_RS28995 Genome accession   NZ_CP017099
Coordinates   6051653..6053797 (-) Length   714 a.a.
NCBI ID   WP_003114575.1    Uniprot ID   P34750
Organism   Pseudomonas aeruginosa strain DN1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6046653..6058797
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BG483_RS28980 (BG483_28020) - 6048299..6049960 (-) 1662 WP_023101642.1 AAA family ATPase -
  BG483_RS28985 (BG483_28025) aroB 6049967..6051073 (-) 1107 WP_003107839.1 3-dehydroquinate synthase -
  BG483_RS28990 (BG483_28030) aroK 6051123..6051641 (-) 519 WP_003095833.1 shikimate kinase AroK -
  BG483_RS28995 (BG483_28035) pilQ 6051653..6053797 (-) 2145 WP_003114575.1 type 4a pilus secretin PilQ Machinery gene
  BG483_RS29000 (BG483_28040) pilP 6053851..6054375 (-) 525 WP_021263517.1 type 4a pilus biogenesis lipoprotein PilP -
  BG483_RS29005 (BG483_28045) pilO 6054372..6054995 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO -
  BG483_RS29010 (BG483_28050) pilN 6054992..6055588 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN -
  BG483_RS29015 (BG483_28055) pilM 6055588..6056652 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 714 a.a.        Molecular weight: 77378.94 Da        Isoelectric Point: 5.2285

>NTDB_id=195585 BG483_RS28995 WP_003114575.1 6051653..6053797(-) (pilQ) [Pseudomonas aeruginosa strain DN1]
MNSGLSRLGIALLAAMFAPALLAADLEKLDVAALPGDRVELKLQFDEPVAAPRGYTIEQPARIALDLPGVQNKLGTKNRE
LSVGNTRSVTVVEAKDRTRLIINLTALSSYTTRVEGNNLFVVVGNSPAGASVASAAPVKASPAPASYAQPIKPKPYVPAG
RAIRNIDFQRGEKGEGNVVIDLSDPTLSPDIQEQGGKIRLDFAKTQLPDALRVRLDVKDFATPVQFVNASAQSDRTSITI
EPSGLYDYLVYQTDNRLTVSIKPMTTEDAERRKKDNFAYTGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVQGNITL
RLQNVPWDQALDLVLKTKGLDKRKLGNVLLVAPADEIAARERQELEAQKQIAELAPLRRELIQVNYAKAADIAKLFQSVT
SDGGQEGKEGGRGSITVDDRTNSIIAYQPQERLDELRRIVSQLDIPVRQVMIEARIVEANVGYDKSLGVRWGGAYHKGNW
SGYGKDGNIGIKDEDGMNCGPIAGSCTFPTTGTSKSPSPFVDLGAKDATSGIGIGFITDNIILDLQLSAMEKTGNGEIVS
QPKVVTSDKETAKILKGSEVPYQEASSSGATSTSFKEAALSLEVTPQITPDNRIIVEVKVTKDAPDYQNMLNGVPPINKN
EVNAKILVNDGETIVIGGVFSNEQSKSVEKVPFLGELPYLGRLFRRDTVTDRKNELLVFLTPRIMNNQAIAIGR

Nucleotide


Download         Length: 2145 bp        

>NTDB_id=195585 BG483_RS28995 WP_003114575.1 6051653..6053797(-) (pilQ) [Pseudomonas aeruginosa strain DN1]
ATGAACAGTGGCCTCTCGCGCCTCGGGATCGCTTTGCTGGCCGCCATGTTCGCGCCGGCATTGCTCGCCGCGGACCTGGA
GAAACTCGACGTGGCGGCACTGCCTGGCGACCGGGTCGAGCTAAAGCTGCAGTTCGACGAGCCCGTCGCCGCGCCGCGTG
GCTATACCATCGAGCAGCCGGCGCGAATCGCCCTCGATCTGCCGGGGGTGCAGAACAAGCTCGGCACCAAGAATCGCGAG
CTGAGCGTGGGCAATACGCGCAGCGTCACGGTGGTCGAGGCGAAGGATCGCACACGGTTGATCATCAACCTGACCGCGCT
GTCGTCCTACACCACTCGGGTGGAAGGCAACAACCTCTTCGTGGTGGTCGGCAATTCGCCGGCCGGCGCCAGCGTCGCTT
CCGCCGCTCCGGTCAAGGCAAGCCCGGCTCCGGCCAGCTATGCCCAGCCGATCAAGCCCAAGCCGTACGTGCCGGCGGGT
CGTGCCATCCGCAACATCGATTTCCAGCGTGGCGAAAAGGGCGAGGGCAACGTGGTCATCGACCTGTCCGACCCGACCTT
GAGCCCGGATATCCAGGAGCAGGGCGGCAAGATCCGCCTGGACTTCGCCAAGACCCAGCTGCCGGATGCGCTGCGGGTTC
GCCTGGACGTCAAGGACTTCGCCACGCCGGTGCAGTTCGTCAACGCCAGCGCGCAATCCGACCGTACCAGCATCACCATC
GAGCCGAGCGGGCTCTACGACTACCTGGTCTACCAGACCGACAATCGCCTGACCGTCAGCATCAAGCCGATGACCACGGA
AGATGCCGAGCGGCGCAAGAAGGACAATTTCGCCTATACCGGCGAGAAACTGTCGCTGAACTTCCAGGACATCGACGTGC
GTTCGGTACTGCAACTGATCGCCGACTTCACCGACCTGAATCTGGTGGCCTCGGATACCGTGCAGGGCAATATCACCCTG
CGCCTGCAGAACGTGCCGTGGGACCAGGCGCTGGACCTGGTACTGAAGACCAAGGGCCTGGACAAGCGCAAGCTCGGCAA
CGTACTGCTGGTGGCCCCGGCCGACGAGATCGCCGCCCGTGAGCGCCAGGAGCTGGAAGCGCAGAAGCAGATCGCCGAGT
TGGCGCCACTGCGCCGGGAACTGATCCAGGTGAACTACGCCAAGGCCGCGGACATCGCCAAGCTGTTCCAGTCGGTGACC
AGCGATGGTGGCCAGGAAGGCAAGGAAGGCGGGCGCGGCTCGATCACCGTCGACGACCGCACCAACAGCATCATCGCCTA
TCAGCCCCAGGAGCGACTGGACGAACTGCGGCGGATCGTTTCGCAGCTGGATATTCCGGTGCGCCAGGTGATGATCGAGG
CACGCATCGTCGAGGCCAACGTCGGTTACGACAAGAGTCTCGGTGTGCGCTGGGGCGGCGCTTATCACAAGGGCAACTGG
AGCGGCTACGGGAAGGACGGCAATATCGGGATCAAGGACGAGGACGGCATGAACTGCGGCCCGATCGCCGGTAGCTGTAC
CTTCCCGACCACGGGTACCAGCAAGTCGCCGTCGCCGTTCGTCGACCTCGGTGCCAAGGATGCTACCTCCGGGATCGGCA
TCGGCTTCATCACCGACAACATCATCCTCGACCTGCAACTGTCGGCGATGGAAAAGACCGGCAACGGCGAGATCGTCTCC
CAACCCAAGGTCGTGACCTCGGACAAGGAAACCGCGAAGATCCTCAAGGGCTCGGAAGTCCCCTACCAGGAGGCCAGTTC
CAGCGGCGCTACCAGCACCTCGTTCAAGGAAGCCGCGCTGTCCCTCGAGGTGACCCCGCAGATCACCCCGGACAACCGGA
TCATCGTCGAAGTGAAGGTGACCAAGGACGCGCCGGATTACCAGAATATGTTGAACGGCGTACCTCCTATCAACAAGAAC
GAGGTCAACGCCAAGATCCTGGTCAACGACGGCGAGACCATCGTCATCGGCGGCGTGTTCAGTAACGAGCAGAGCAAGTC
GGTGGAGAAGGTGCCGTTCCTCGGCGAACTGCCTTACCTGGGGCGGCTGTTCCGGCGTGATACCGTCACCGACCGGAAAA
ACGAACTTCTGGTTTTCCTGACACCGCGGATCATGAATAATCAGGCCATCGCAATCGGTCGCTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(549-705)

AMIN domain (AMIN)

(38-122)

Secretin and TonB N terminus short domain (STN)

(307-353)

Bacterial type II/III secretion system short domain (Secretin_N)

(381-449)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 6VE2
  PDB 6VE3
  PDB 6VE4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

99.72

100

0.997
  pilQ Acinetobacter baumannii strain A118

40.699

100

0.408
  pilQ Acinetobacter baumannii D1279779

40.699

100

0.408
  comQ Acinetobacter baylyi ADP1

40.967

98.459

0.403
  pilQ Legionella pneumophila strain Lp02

38.102

95.938

0.366
  pilQ Legionella pneumophila strain ERS1305867

37.956

95.938

0.364

Multiple sequence alignment