Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   A6B39_RS03365 Genome accession   NZ_CP016606
Coordinates   671983..673374 (+) Length   463 a.a.
NCBI ID   WP_176669724.1    Uniprot ID   -
Organism   Mannheimia granulomatis strain BJ1680.3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 666983..678374
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A6B39_RS03335 (A6B39_03290) - 667036..667797 (-) 762 WP_176669719.1 TSUP family transporter -
  A6B39_RS03340 (A6B39_03295) proB 667797..668888 (-) 1092 WP_176669720.1 glutamate 5-kinase -
  A6B39_RS03345 (A6B39_03300) - 669003..669491 (+) 489 WP_176669721.1 dihydrofolate reductase -
  A6B39_RS03350 (A6B39_03305) - 669621..669854 (+) 234 WP_027074413.1 YgjV family protein -
  A6B39_RS03355 (A6B39_03310) radA 669920..671299 (-) 1380 WP_176669722.1 DNA repair protein RadA -
  A6B39_RS03360 (A6B39_03315) pilA 671484..671945 (+) 462 WP_176669723.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  A6B39_RS03365 (A6B39_03320) pilB 671983..673374 (+) 1392 WP_176669724.1 GspE/PulE family protein Machinery gene
  A6B39_RS03370 (A6B39_03325) pilC 673367..674563 (+) 1197 WP_176669725.1 type II secretion system F family protein Machinery gene
  A6B39_RS03375 (A6B39_03330) - 674567..675202 (+) 636 WP_176669726.1 prepilin peptidase -
  A6B39_RS03380 (A6B39_03335) - 675296..675604 (+) 309 WP_176669727.1 helix-turn-helix domain-containing protein -
  A6B39_RS03385 (A6B39_03340) coaE 675614..676252 (+) 639 WP_176669728.1 dephospho-CoA kinase -
  A6B39_RS03390 (A6B39_03345) yacG 676234..676419 (+) 186 WP_176669729.1 DNA gyrase inhibitor YacG -
  A6B39_RS03395 (A6B39_03350) holA 676420..677445 (-) 1026 WP_176669730.1 DNA polymerase III subunit delta -
  A6B39_RS03400 (A6B39_03355) lptE 677489..677983 (-) 495 WP_027074423.1 LPS assembly lipoprotein LptE -

Sequence

Protein


Download         Length: 463 a.a.        Molecular weight: 52636.47 Da        Isoelectric Point: 5.1562

>NTDB_id=189623 A6B39_RS03365 WP_176669724.1 671983..673374(+) (pilB) [Mannheimia granulomatis strain BJ1680.3]
MEYSVTDLNSQQIIEISASQWQKNCEDKLILLRYLAVPLQENEDKLWLAIDDMQNLNACEIFAFIAHKQIEPVLISSEEL
KFLLNALSPELNHIQSEIYEENRAYQAENDSAIDIHDPIIQILDNLFKYCLQKEASDIHLEPQKEQLRVRLRIDGVLHLY
KTFPLSLSSRLISRIKLLAKLDISESRLPQDGQFHFKTRLAESLDFRVSTLPTLWGEKLVLRLQKNKPTHFDFLDLGFNS
SQKMTLLNALQQPQGLILVTGPIGSGKSITLYSALSYLNSNEKHILTAEDPIEIEIDGIIQTQVNRGIDLDFSQLLRTFL
RQDPDVIMLGEIRDEESAEIALRAAQTGHLVLSTLHTNDAPSAIERLLQLGIKEYELKNSLLLVVAQRLLRRLCVKCDGI
GCEHCYQGYKGRIGVYQLLSRTAKNFEKTTACLDFETLLDAAKEKLAESKTDQAEILRVLGNG

Nucleotide


Download         Length: 1392 bp        

>NTDB_id=189623 A6B39_RS03365 WP_176669724.1 671983..673374(+) (pilB) [Mannheimia granulomatis strain BJ1680.3]
ATGGAATATTCAGTTACCGATCTTAATAGCCAACAAATCATTGAAATTTCCGCTTCACAATGGCAGAAAAACTGTGAAGA
TAAGCTGATCTTATTGCGATATCTAGCAGTGCCTTTGCAGGAAAATGAAGATAAATTATGGCTGGCGATAGATGATATGC
AAAATCTGAATGCGTGCGAAATTTTTGCCTTTATTGCACACAAACAGATTGAACCTGTTTTGATTTCCTCTGAAGAATTA
AAGTTTTTACTAAATGCACTTTCTCCTGAACTAAATCATATTCAAAGCGAAATTTATGAGGAAAATCGGGCTTACCAAGC
CGAAAATGATTCGGCAATAGATATTCATGACCCGATCATCCAAATTTTAGATAACTTGTTTAAGTATTGTTTACAGAAAG
AGGCCTCTGATATCCATTTAGAACCGCAAAAAGAACAATTGCGTGTGCGACTGCGTATTGATGGTGTACTGCATTTGTAT
AAAACATTTCCGCTAAGTTTGTCCAGCCGATTGATTTCTCGTATTAAATTGCTGGCTAAATTGGATATTAGCGAAAGCCG
CTTGCCACAAGACGGACAGTTTCATTTTAAAACTAGGCTTGCCGAAAGTTTAGATTTTCGTGTCTCCACATTACCCACCC
TTTGGGGGGAGAAGCTCGTCTTGCGGTTACAAAAAAACAAACCAACTCATTTTGATTTTCTGGATCTCGGCTTTAACTCA
AGTCAAAAAATGACTTTACTGAATGCTTTACAGCAGCCACAAGGGCTGATTTTAGTGACCGGTCCGATAGGTAGTGGGAA
AAGTATCACGCTTTATAGTGCCTTGAGCTATTTGAACAGTAACGAAAAGCATATTTTAACAGCAGAAGATCCAATAGAAA
TTGAGATAGACGGCATTATTCAAACTCAAGTTAATCGTGGGATAGATCTTGATTTCAGCCAACTGCTACGTACCTTTCTA
CGGCAAGATCCTGATGTCATTATGCTCGGTGAAATTCGTGATGAAGAGAGTGCTGAAATTGCCTTGAGAGCAGCTCAAAC
TGGGCATTTGGTACTTTCAACTTTGCATACCAATGATGCCCCTTCTGCGATTGAACGCTTACTGCAACTTGGCATAAAAG
AGTACGAATTAAAAAATTCATTATTGTTGGTGGTAGCACAACGCTTGTTACGCCGACTTTGTGTGAAATGTGATGGTATA
GGTTGTGAGCATTGCTATCAGGGTTACAAAGGGAGGATTGGGGTCTATCAGCTTCTAAGCCGAACGGCAAAAAATTTTGA
AAAAACGACCGCTTGTTTAGACTTTGAAACTCTACTTGATGCAGCCAAAGAGAAATTGGCGGAAAGTAAAACCGATCAAG
CCGAAATTTTAAGGGTGCTAGGTAATGGCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(30-87)

Type II/IV secretion system protein (T2SSE)

(130-392)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

71.15

99.568

0.708
  pilB Haemophilus influenzae Rd KW20

56.388

98.056

0.553
  pilB Haemophilus influenzae 86-028NP

56.291

97.84

0.551
  pilB Vibrio parahaemolyticus RIMD 2210633

40

100

0.432
  pilB Vibrio campbellii strain DS40M4

41.079

100

0.428
  pilB Vibrio cholerae strain A1552

40.041

100

0.417
  pilB Acinetobacter baylyi ADP1

36.437

100

0.389
  pilB Legionella pneumophila strain ERS1305867

40.455

95.032

0.384
  pilB Acinetobacter baumannii D1279779

38.341

96.328

0.369

Multiple sequence alignment