Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   A6B39_RS03360 Genome accession   NZ_CP016606
Coordinates   671484..671945 (+) Length   153 a.a.
NCBI ID   WP_176669723.1    Uniprot ID   -
Organism   Mannheimia granulomatis strain BJ1680.3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 666484..676945
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A6B39_RS03335 (A6B39_03290) - 667036..667797 (-) 762 WP_176669719.1 TSUP family transporter -
  A6B39_RS03340 (A6B39_03295) proB 667797..668888 (-) 1092 WP_176669720.1 glutamate 5-kinase -
  A6B39_RS03345 (A6B39_03300) - 669003..669491 (+) 489 WP_176669721.1 dihydrofolate reductase -
  A6B39_RS03350 (A6B39_03305) - 669621..669854 (+) 234 WP_027074413.1 YgjV family protein -
  A6B39_RS03355 (A6B39_03310) radA 669920..671299 (-) 1380 WP_176669722.1 DNA repair protein RadA -
  A6B39_RS03360 (A6B39_03315) pilA 671484..671945 (+) 462 WP_176669723.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  A6B39_RS03365 (A6B39_03320) pilB 671983..673374 (+) 1392 WP_176669724.1 GspE/PulE family protein Machinery gene
  A6B39_RS03370 (A6B39_03325) pilC 673367..674563 (+) 1197 WP_176669725.1 type II secretion system F family protein Machinery gene
  A6B39_RS03375 (A6B39_03330) - 674567..675202 (+) 636 WP_176669726.1 prepilin peptidase -
  A6B39_RS03380 (A6B39_03335) - 675296..675604 (+) 309 WP_176669727.1 helix-turn-helix domain-containing protein -
  A6B39_RS03385 (A6B39_03340) coaE 675614..676252 (+) 639 WP_176669728.1 dephospho-CoA kinase -
  A6B39_RS03390 (A6B39_03345) yacG 676234..676419 (+) 186 WP_176669729.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 153 a.a.        Molecular weight: 15936.12 Da        Isoelectric Point: 8.0982

>NTDB_id=189622 A6B39_RS03360 WP_176669723.1 671484..671945(+) (pilA) [Mannheimia granulomatis strain BJ1680.3]
MQKISLFRPLAPAFTLIELMIVIAIIAILATVAIPSYNSYTQKAALSELLQASASYKSDVELCIYHTGALTNCSGGSNGI
QANKTSVSGTKYLNSISVANGVITVSGKGSLNGYGYTLTPTYSNNTISWSASCSGTDTSLFPANFCTATTPQG

Nucleotide


Download         Length: 462 bp        

>NTDB_id=189622 A6B39_RS03360 WP_176669723.1 671484..671945(+) (pilA) [Mannheimia granulomatis strain BJ1680.3]
ATGCAAAAAATTTCACTTTTTCGACCGCTTGCACCGGCATTTACTTTAATTGAGTTGATGATCGTGATTGCGATTATTGC
CATTCTTGCTACTGTTGCGATCCCCTCTTACAACTCTTACACCCAAAAAGCTGCGTTATCGGAATTATTGCAAGCCTCAG
CTTCCTATAAATCGGATGTGGAACTCTGTATTTACCACACAGGGGCTTTAACCAACTGCTCGGGCGGTTCAAATGGTATT
CAAGCCAATAAAACCTCAGTGAGTGGCACTAAATACTTGAATTCAATTAGTGTAGCAAATGGGGTGATTACAGTGAGTGG
AAAAGGCTCATTAAACGGCTATGGCTACACCCTAACGCCAACATATAGCAATAATACGATCAGCTGGTCAGCAAGCTGCT
CAGGCACAGATACCAGCCTCTTTCCTGCGAATTTCTGTACGGCAACAACACCTCAAGGTTAG

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(13-33)

Pilin (bacterial filament) (Pilin)

(43-144)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

70.946

96.732

0.686
  pilA Haemophilus influenzae 86-028NP

57.718

97.386

0.562
  pilA Haemophilus influenzae Rd KW20

54.362

97.386

0.529

Multiple sequence alignment