Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   A6A10_RS03585 Genome accession   NZ_CP016604
Coordinates   775161..776552 (-) Length   463 a.a.
NCBI ID   WP_229583618.1    Uniprot ID   A0A420XJ79
Organism   Otariodibacter oris strain Baika1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 770161..781552
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A6A10_RS03565 (A6A10_03545) - 771240..771746 (+) 507 WP_121121914.1 CvpA family protein -
  A6A10_RS03570 (A6A10_03550) purF 771755..773272 (+) 1518 WP_121121912.1 amidophosphoribosyltransferase -
  A6A10_RS03575 (A6A10_03555) - 773323..773724 (-) 402 WP_121121910.1 prepilin peptidase -
  A6A10_RS03580 (A6A10_03560) - 773981..775168 (-) 1188 WP_121121908.1 type II secretion system F family protein -
  A6A10_RS03585 (A6A10_03565) pilB 775161..776552 (-) 1392 WP_229583618.1 GspE/PulE family protein Machinery gene
  A6A10_RS03590 (A6A10_03570) pilA 776580..777023 (-) 444 WP_121121906.1 pilin Machinery gene
  A6A10_RS03595 (A6A10_03575) radA 777236..778615 (+) 1380 WP_121121904.1 DNA repair protein RadA -
  A6A10_RS03600 (A6A10_03580) - 778688..779014 (-) 327 WP_121121902.1 DUF496 family protein -
  A6A10_RS03605 (A6A10_03585) mepA 779205..780032 (+) 828 WP_121121900.1 penicillin-insensitive murein endopeptidase -

Sequence

Protein


Download         Length: 463 a.a.        Molecular weight: 52463.11 Da        Isoelectric Point: 6.2860

>NTDB_id=189582 A6A10_RS03585 WP_229583618.1 775161..776552(-) (pilB) [Otariodibacter oris strain Baika1]
MTSMYSVCEISSQQIFTIANELWQKNCKNKHILQRYLAIPVKETDNTLWLAIDDQNNLNASDSFAFIHNKKIEQIIISSE
ELKYLLNELSPNHEITNELADINYVSENTSNKPDYNDPIIQILDNLFKFCLQQNASDIHIEPAKDKLNIRLRIDGLLQVY
KTLPQHLANRLVLKIKLLANLDISESRQPQDGQFSFTTVLSESLDFRVSTLPTYHGEKLVLRLQKDKPVQLDFLTLGFTP
QQKEILLHALSQPQGLILVTGPTGSGKSITLYTSLNHLNQTDKHILTAEDPIEIEIEGLIQTQVNSSLDLTFSKLLKTFL
RQDPDIIMLGEIRDPESAKIALSAAQTGHLVLSTLHTNDAPSAIDRLVQLGIDEYEIRNSLLLVIAQRLVRKLCEKCLGK
SCQYCYQGYKGRIGVYQCLSRSAKEFNKLTACLDYSSLYLSAKQKVESNQTNLAEIQRVLGYD

Nucleotide


Download         Length: 1392 bp        

>NTDB_id=189582 A6A10_RS03585 WP_229583618.1 775161..776552(-) (pilB) [Otariodibacter oris strain Baika1]
ATGACCTCTATGTATTCAGTTTGCGAAATTAGCTCACAACAAATTTTTACAATCGCAAATGAACTGTGGCAGAAAAATTG
TAAAAACAAGCATATTTTACAACGCTATTTAGCCATCCCTGTTAAAGAAACGGATAATACGTTGTGGCTTGCTATTGATG
ATCAAAATAATTTGAATGCCAGTGATAGTTTCGCCTTTATACACAATAAAAAAATTGAGCAGATTATTATTTCATCTGAA
GAATTAAAATATTTGCTCAATGAACTCTCACCCAATCATGAAATCACCAATGAATTGGCCGACATAAATTATGTATCTGA
GAATACCTCGAATAAGCCAGACTACAATGATCCAATTATTCAGATCCTTGATAATCTTTTTAAATTTTGTTTGCAACAAA
ATGCCTCAGATATACACATAGAACCTGCAAAAGATAAATTGAACATACGCCTAAGAATAGATGGGCTACTGCAAGTTTAT
AAAACACTACCTCAGCATTTAGCGAATCGATTAGTGTTAAAGATTAAACTCCTCGCAAATCTTGATATTAGTGAATCTCG
CCAACCGCAAGATGGTCAATTTAGTTTTACCACCGTACTGTCAGAATCTTTGGATTTTCGTGTTTCAACATTGCCAACCT
ATCACGGAGAAAAATTAGTCTTACGTTTACAAAAAGATAAGCCCGTTCAACTTGATTTCCTTACTTTAGGTTTTACTCCA
CAACAAAAGGAAATCTTATTACACGCATTAAGCCAACCTCAAGGTCTGATACTCGTTACAGGTCCAACAGGAAGTGGTAA
AAGTATTACCCTATATACTTCATTAAATCACTTAAACCAAACAGACAAACATATTCTCACGGCTGAAGATCCCATAGAAA
TTGAAATTGAGGGGTTAATTCAAACCCAAGTCAATTCCTCTTTAGATCTTACATTTAGTAAGTTGTTAAAGACTTTTTTA
AGGCAAGATCCCGATATTATTATGCTAGGCGAAATCCGAGATCCTGAAAGTGCCAAAATAGCATTAAGTGCGGCCCAAAC
GGGACATCTAGTCCTATCAACACTGCATACCAATGATGCTCCCTCTGCGATTGATCGACTGGTACAGCTAGGTATTGATG
AATATGAAATTAGAAATTCTCTATTATTAGTTATCGCTCAAAGACTTGTTAGAAAACTATGCGAAAAATGCCTCGGTAAA
TCTTGCCAGTACTGCTATCAAGGCTATAAAGGTCGAATTGGTGTTTATCAATGCTTATCCCGTTCTGCAAAAGAATTTAA
CAAACTGACCGCTTGTTTAGACTACTCATCTTTATATCTTAGTGCGAAACAAAAGGTTGAATCGAACCAAACTAATCTAG
CTGAAATTCAAAGAGTACTAGGGTATGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(29-94)

Type II/IV secretion system protein (T2SSE)

(131-392)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A420XJ79

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

69.197

99.568

0.689
  pilB Haemophilus influenzae Rd KW20

55.432

97.408

0.54
  pilB Haemophilus influenzae 86-028NP

55.211

97.408

0.538
  pilB Vibrio parahaemolyticus RIMD 2210633

38.923

100

0.406
  pilB Acinetobacter baylyi ADP1

38.241

100

0.404
  pilB Legionella pneumophila strain ERS1305867

37.959

100

0.402
  pilB Vibrio campbellii strain DS40M4

38.302

100

0.4
  pilB Vibrio cholerae strain A1552

37.397

100

0.391
  pilB Acinetobacter baumannii D1279779

36.42

100

0.382
  pilB/pilB1 Synechocystis sp. PCC 6803

34.008

100

0.363

Multiple sequence alignment