Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   VSVS05_RS12470 Genome accession   NZ_CP016414
Coordinates   2738137..2739855 (-) Length   572 a.a.
NCBI ID   WP_156785342.1    Uniprot ID   -
Organism   Vibrio scophthalmi strain VS-05     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2733137..2744855
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VSVS05_RS12450 (VSVS05_02546) - 2733773..2734627 (-) 855 WP_005597170.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  VSVS05_RS12455 (VSVS05_02547) - 2734752..2736224 (-) 1473 WP_065545770.1 SPOR domain-containing protein -
  VSVS05_RS12460 (VSVS05_02548) aroB 2736320..2737423 (-) 1104 WP_065545771.1 3-dehydroquinate synthase -
  VSVS05_RS12465 (VSVS05_02549) aroK 2737454..2737972 (-) 519 WP_005597180.1 shikimate kinase AroK -
  VSVS05_RS12470 (VSVS05_02550) pilQ 2738137..2739855 (-) 1719 WP_156785342.1 type IV pilus secretin PilQ Machinery gene
  VSVS05_RS12475 (VSVS05_02551) - 2739852..2740430 (-) 579 WP_065545773.1 pilus assembly protein PilP -
  VSVS05_RS12480 (VSVS05_02552) - 2740417..2741007 (-) 591 WP_065545774.1 type 4a pilus biogenesis protein PilO -
  VSVS05_RS12485 (VSVS05_02553) - 2741000..2741602 (-) 603 WP_065545775.1 PilN domain-containing protein -
  VSVS05_RS12490 (VSVS05_02554) pilM 2741592..2742563 (-) 972 WP_065545776.1 type IV pilus biogenesis protein PilM -

Sequence

Protein


Download         Length: 572 a.a.        Molecular weight: 62511.19 Da        Isoelectric Point: 4.5164

>NTDB_id=188165 VSVS05_RS12470 WP_156785342.1 2738137..2739855(-) (pilQ) [Vibrio scophthalmi strain VS-05]
MIQGWKVTNQKRMRCLQVICVVIVALLTAQPQAWSMSNQLLSVDFRLDDTNRGKVMLQLSSDQLALDIQEDPQGLVIELF
DTSVDDALLSRFDVADFGNNVQTMEVFRLDDRVRVHIQIEGRFAYQHTLDGGLLELVVTEAESEGKHDLSMLSKASANKI
AINFQDISVRNVLQLIAEYNDFNLVVSDAVTGNLTLRLDDVPWPQVLDIILQVKGLDKRIEGNVVLVAPKAEFDLREQQR
LENARMAEQLSELHSDIIAVNFANASDIAEMIGGSGAISMLSERGSITVDERTNALLIRDLPSHIEMVRSVVGSLDVPVK
QVQIEARIVTVNQGSLDELGVRWSLNSTTGNLGATLEGNLNQRDSDGLLSPGDNIDDFLNVNLAATAGHSSSLAFQVAKL
GSSTLLDLELSALQSESKAEVISSPRLITTNKKPAYIEQGTDIPFLESTSSGASSVSFKKAVLSLKVTPQITPDNRLILD
LSVTQDRPGEVVKVGTGEAVSINTQRIGTQVLVDNGETIVLGGIYQHSVSDTVDKVPLLGDIPYLGALFRRSYQRMGKSE
LLIFVTPKVVMQ

Nucleotide


Download         Length: 1719 bp        

>NTDB_id=188165 VSVS05_RS12470 WP_156785342.1 2738137..2739855(-) (pilQ) [Vibrio scophthalmi strain VS-05]
ATGATTCAAGGATGGAAGGTGACTAATCAAAAGAGGATGAGATGCTTACAGGTTATCTGCGTTGTTATTGTTGCCCTGCT
CACGGCGCAGCCCCAAGCTTGGTCGATGAGCAATCAACTGCTTTCGGTTGATTTTCGTTTGGATGACACGAACCGCGGCA
AAGTGATGTTGCAGCTTAGCAGTGATCAACTCGCGCTGGATATTCAGGAGGATCCACAAGGTTTGGTGATTGAGCTGTTT
GATACCTCGGTTGATGATGCGCTTTTATCACGCTTTGACGTGGCTGACTTCGGTAACAATGTTCAAACCATGGAGGTGTT
TCGTTTGGACGACCGCGTGCGAGTGCACATTCAGATTGAGGGCCGCTTTGCTTATCAGCATACTCTTGATGGAGGTCTGT
TAGAGTTAGTGGTCACCGAGGCTGAAAGCGAGGGAAAACACGACCTGAGTATGCTGAGCAAAGCCTCTGCCAATAAGATC
GCGATTAATTTTCAAGACATTTCGGTGCGCAATGTCTTGCAGTTGATTGCTGAATATAATGATTTTAATTTAGTCGTGTC
AGATGCGGTGACGGGGAATCTCACCTTGCGACTGGATGATGTGCCATGGCCACAAGTGCTAGACATTATCTTGCAAGTTA
AAGGGTTAGATAAACGAATCGAAGGTAATGTCGTTCTGGTGGCTCCAAAAGCAGAATTCGATCTGCGCGAGCAGCAACGA
TTAGAGAATGCCCGTATGGCAGAGCAATTAAGCGAGTTGCATTCTGACATCATTGCCGTCAACTTTGCTAATGCCAGTGA
CATTGCAGAGATGATTGGCGGTAGTGGCGCCATCAGTATGTTGTCTGAACGTGGTTCGATTACCGTTGATGAGCGCACCA
ACGCGTTACTCATCCGTGATTTGCCAAGCCATATTGAGATGGTTCGCAGCGTGGTGGGTTCGCTTGATGTCCCTGTTAAG
CAAGTACAGATAGAAGCGCGCATTGTCACGGTTAACCAAGGCAGTTTGGATGAGCTTGGGGTGCGTTGGAGCTTAAATTC
AACCACCGGCAACCTTGGCGCTACGCTAGAGGGCAATCTCAATCAGCGAGACTCGGATGGTCTGTTATCGCCAGGGGATA
ACATCGATGACTTTCTTAACGTTAATTTAGCCGCCACCGCGGGTCATTCATCAAGCCTTGCGTTCCAAGTGGCCAAGTTA
GGCTCCTCAACCTTGTTGGATTTAGAACTGTCGGCACTGCAAAGTGAATCGAAAGCAGAGGTCATTTCTAGCCCACGCTT
GATCACCACCAATAAAAAACCAGCCTATATTGAGCAGGGCACGGATATTCCTTTCCTTGAATCCACCTCAAGTGGTGCCA
GTAGTGTTTCATTTAAAAAAGCGGTGTTGAGTTTAAAAGTCACGCCACAAATTACGCCGGATAACCGATTGATTCTTGAT
TTGAGTGTGACCCAAGATCGACCGGGCGAAGTGGTCAAAGTGGGTACGGGAGAGGCGGTATCGATTAATACTCAACGCAT
TGGTACACAAGTATTAGTTGATAACGGGGAGACCATCGTGCTGGGCGGGATATATCAACACAGTGTGAGCGATACTGTCG
ATAAAGTTCCTCTGCTTGGCGACATCCCATATTTAGGCGCACTGTTTCGTCGCAGCTACCAACGAATGGGCAAAAGTGAG
CTGCTGATTTTCGTCACTCCCAAAGTTGTTATGCAGTAA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(412-570)

Bacterial type II/III secretion system short domain (Secretin_N)

(257-321)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

64.051

95.804

0.614
  pilQ Vibrio cholerae strain A1552

63.956

94.58

0.605
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

63.956

94.58

0.605
  pilQ Legionella pneumophila strain ERS1305867

41.316

95.629

0.395
  pilQ Legionella pneumophila strain Lp02

41.316

95.629

0.395
  pilQ Pseudomonas aeruginosa PAK

39.115

98.776

0.386
  pilQ Acinetobacter baumannii D1279779

35.025

100

0.362
  pilQ Acinetobacter baumannii strain A118

35.025

100

0.362

Multiple sequence alignment