Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   VSVS05_RS01030 Genome accession   NZ_CP016414
Coordinates   195336..197018 (-) Length   560 a.a.
NCBI ID   WP_065545002.1    Uniprot ID   A0A1C7F7R8
Organism   Vibrio scophthalmi strain VS-05     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 190336..202018
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VSVS05_RS01000 (VSVS05_00204) rplS 190861..191214 (+) 354 WP_005593972.1 50S ribosomal protein L19 -
  VSVS05_RS01005 (VSVS05_00205) yacG 191426..191620 (-) 195 WP_005593974.1 DNA gyrase inhibitor YacG -
  VSVS05_RS01010 (VSVS05_00206) zapD 191696..192436 (-) 741 WP_005593976.1 cell division protein ZapD -
  VSVS05_RS01015 (VSVS05_00207) coaE 192471..193079 (-) 609 WP_065430956.1 dephospho-CoA kinase -
  VSVS05_RS01020 (VSVS05_00208) pilD 193084..193953 (-) 870 WP_065545000.1 prepilin peptidase Machinery gene
  VSVS05_RS01025 (VSVS05_00209) pilC 194059..195282 (-) 1224 WP_065545001.1 type II secretion system F family protein Machinery gene
  VSVS05_RS01030 (VSVS05_00210) pilB 195336..197018 (-) 1683 WP_065545002.1 type IV-A pilus assembly ATPase PilB Machinery gene
  VSVS05_RS01035 (VSVS05_00211) pilA 197018..197476 (-) 459 WP_065430953.1 pilin Machinery gene
  VSVS05_RS01040 (VSVS05_00212) mutS 197739..200306 (-) 2568 WP_065545003.1 DNA mismatch repair protein MutS -
  VSVS05_RS01045 (VSVS05_00213) pncC 200391..200879 (+) 489 WP_005593984.1 nicotinamide-nucleotide amidase -

Sequence

Protein


Download         Length: 560 a.a.        Molecular weight: 61817.92 Da        Isoelectric Point: 6.9266

>NTDB_id=188140 VSVS05_RS01030 WP_065545002.1 195336..197018(-) (pilB) [Vibrio scophthalmi strain VS-05]
MSHHLATLLSQQGLLAHTQVGQPPFTSNHPSGFDPSILEFISADKLAAVVSQLFKLPQVNIAHYEYRTVCEELGLSTLIQ
KHLSLPLAKTNGTLTLAVVDPTLDVIEDEFRFATGLQIELVVTDYNAIKKAIYALYGRSLQSTTTAKKEINALELAQLVN
VSEEEFGREEDLSLDDAPVSRYIHQILLEAIRKGASDIHFEPYEKHYRIRLRCDGLLVESHQPASQLGRRLSTRLKILAN
LDIAERRRPQDGRSKVKLNADSAVDMRISTLPTLWGEKVVLRLLESGINLLDIDRLGYSHQQKQHYLKALKQPQGLILFT
GPTGSGKTISLYSGLNLLNTTQVNIATAEDPVEINLSGINQVQIQPQIGFDFACALRSFLRQDPDIVMVGEIRDVETADI
AIKASQTGHLVLSTLHTNSAADTIVRLASMGIKTYDLAASLTLVMAQRLVRKLCAHCKEPAKRNIDAKDDLLGPVTDQQY
QANQHGCTQCNQGYQGRIGLFEVLPINAKLQQAILQCATAIEIETLAKNEGMMSLQQSGIAALNAGKTSRQELQRVLLFG

Nucleotide


Download         Length: 1683 bp        

>NTDB_id=188140 VSVS05_RS01030 WP_065545002.1 195336..197018(-) (pilB) [Vibrio scophthalmi strain VS-05]
ATGAGTCATCATTTAGCGACGCTGCTTTCTCAACAAGGCCTACTTGCTCACACGCAAGTAGGCCAGCCACCTTTTACCTC
AAACCATCCTTCTGGCTTTGATCCGTCGATACTAGAATTCATTAGCGCAGATAAATTAGCCGCTGTGGTAAGCCAATTAT
TCAAACTGCCACAGGTTAATATTGCTCACTATGAGTATCGTACGGTTTGTGAAGAACTGGGGTTGAGTACACTGATCCAA
AAACACCTTTCATTACCACTTGCCAAAACCAATGGCACGCTCACGCTGGCAGTGGTCGATCCGACACTGGATGTGATTGA
AGACGAGTTTCGTTTTGCGACTGGATTGCAGATTGAATTAGTGGTGACTGACTACAATGCTATCAAGAAGGCCATTTATG
CCCTCTATGGCCGAAGCCTTCAATCCACCACAACCGCGAAGAAAGAGATCAATGCCCTCGAACTGGCACAGCTAGTGAAT
GTCAGCGAAGAGGAGTTTGGTCGAGAAGAAGATTTGAGCCTAGATGACGCCCCCGTCAGTCGCTACATTCATCAGATATT
ACTCGAGGCGATACGCAAAGGGGCTTCTGATATTCATTTTGAACCGTATGAAAAGCATTACCGGATTCGCCTACGCTGCG
ACGGACTACTCGTAGAAAGCCACCAGCCAGCTTCTCAACTTGGCCGCCGCCTCTCCACACGGTTGAAAATATTAGCCAAC
TTAGATATCGCTGAACGTCGACGACCGCAAGATGGCAGAAGCAAGGTGAAGCTAAATGCTGATAGCGCCGTGGATATGCG
AATCTCGACGCTACCCACTTTATGGGGTGAAAAGGTGGTGCTGCGATTATTGGAAAGCGGTATCAACCTGCTTGATATTG
ACCGACTCGGCTATAGCCACCAGCAAAAACAGCACTACTTAAAAGCACTCAAACAACCACAAGGGCTGATATTGTTTACT
GGCCCAACAGGCAGCGGTAAAACCATCTCACTCTATAGTGGGCTAAATCTTCTTAACACGACGCAAGTCAATATTGCCAC
CGCAGAAGATCCAGTAGAGATCAATCTCAGCGGTATCAATCAAGTCCAAATTCAACCGCAAATCGGCTTCGACTTCGCTT
GCGCACTGCGATCTTTTCTTCGTCAAGATCCAGATATTGTTATGGTCGGCGAAATTCGTGATGTTGAAACCGCTGACATT
GCGATCAAAGCGTCACAAACGGGCCACTTGGTTCTTTCCACTTTGCACACCAACTCCGCAGCTGACACCATCGTTCGTTT
AGCGAGTATGGGGATTAAAACTTATGATCTTGCCGCCTCACTGACGCTGGTGATGGCCCAGCGCTTAGTCAGAAAACTCT
GCGCGCATTGCAAAGAGCCAGCAAAACGAAACATCGATGCTAAGGACGATTTACTCGGACCCGTCACTGATCAGCAGTAT
CAAGCCAATCAGCACGGTTGTACTCAATGTAATCAGGGTTATCAAGGCCGAATAGGCTTGTTTGAAGTACTGCCAATAAA
CGCCAAACTGCAACAAGCGATATTGCAATGCGCGACTGCCATTGAGATTGAAACGCTCGCCAAAAATGAAGGCATGATGA
GCTTACAACAATCCGGTATTGCCGCACTCAACGCTGGCAAAACCAGTCGCCAAGAATTACAACGCGTGTTGCTATTTGGC
TAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(39-139)

Type II/IV secretion system protein (T2SSE)

(185-452)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1C7F7R8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio parahaemolyticus RIMD 2210633

58.865

100

0.593
  pilB Vibrio cholerae strain A1552

58.259

100

0.586
  pilB Vibrio campbellii strain DS40M4

57.194

100

0.575
  pilB Legionella pneumophila strain ERS1305867

46.328

94.821

0.439
  pilB Acinetobacter baumannii D1279779

44.444

93.214

0.414
  pilB Acinetobacter baylyi ADP1

43.893

93.571

0.411
  pilF Neisseria gonorrhoeae MS11

43.378

93.036

0.404

Multiple sequence alignment