Detailed information NTG0188138

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: VSVS05_RS01020
• Genome accession: NZ_CP016414
• Coordinates: 193084..193953 (-)
• Length: 289 a.a.
• NCBI ID: WP_065545000.1
• Uniprot ID: A0A1C7F5Z7
• Organism: Vibrio scophthalmi strain VS-05
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 188084..198953

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
VSVS05_RS00985 (VSVS05_00201)	rpsP	189207..189455 (+)	249	WP_005593968.1	30S ribosomal protein S16	-
VSVS05_RS00990 (VSVS05_00202)	rimM	189480..190034 (+)	555	WP_005593970.1	ribosome maturation factor RimM	-
VSVS05_RS00995 (VSVS05_00203)	trmD	190062..190811 (+)	750	WP_005593971.1	tRNA (guanosine(37)-N1)-methyltransferase TrmD	-
VSVS05_RS01000 (VSVS05_00204)	rplS	190861..191214 (+)	354	WP_005593972.1	50S ribosomal protein L19	-
VSVS05_RS01005 (VSVS05_00205)	yacG	191426..191620 (-)	195	WP_005593974.1	DNA gyrase inhibitor YacG	-
VSVS05_RS01010 (VSVS05_00206)	zapD	191696..192436 (-)	741	WP_005593976.1	cell division protein ZapD	-
VSVS05_RS01015 (VSVS05_00207)	coaE	192471..193079 (-)	609	WP_065430956.1	dephospho-CoA kinase	-
VSVS05_RS01020 (VSVS05_00208)	pilD	193084..193953 (-)	870	WP_065545000.1	prepilin peptidase	Machinery gene
VSVS05_RS01025 (VSVS05_00209)	pilC	194059..195282 (-)	1224	WP_065545001.1	type II secretion system F family protein	Machinery gene
VSVS05_RS01030 (VSVS05_00210)	pilB	195336..197018 (-)	1683	WP_065545002.1	type IV-A pilus assembly ATPase PilB	Machinery gene
VSVS05_RS01035 (VSVS05_00211)	pilA	197018..197476 (-)	459	WP_065430953.1	pilin	Machinery gene

Sequence

Protein

• Length: 289 a.a.
• Molecular weight: 32382.69 Da
• Isoelectric Point: 7.5693

>NTDB_id=188138 VSVS05_RS01020 WP_065545000.1 193084..193953(-) (pilD) [Vibrio scophthalmi strain VS-05]
MELFVYYPWLFPLLATVFGLIVGSFLNVVIYRLPIMMEREWRHDCAESFPEYKIEVPQETFNLSVPRSACPSCHTQIRAI
DNIPVISWLLLKGKCHNCQSKISARYPLIELLTGGLCLLVATTLPASYFTIALLFFTFVLVAATFIDLDTMLLPDSLTLP
LMWSGIALALFGISPVSLQNSVIGAMAGYLCLWSVYWAFKLITKKEGMGYGDFKLLAALGAWLGWQYLPMIILVSSLVGL
VFGLIQLRLQSKGIDKAFPFGPYLAIAGWIAMLWGEQILHGYYRFLLGA

Nucleotide

• Length: 870 bp

>NTDB_id=188138 VSVS05_RS01020 WP_065545000.1 193084..193953(-) (pilD) [Vibrio scophthalmi strain VS-05]
ATGGAACTGTTCGTTTATTACCCTTGGCTCTTCCCACTTCTTGCGACGGTGTTTGGTCTTATCGTTGGCAGCTTTCTCAA
TGTGGTCATCTACCGCTTACCGATTATGATGGAGCGCGAGTGGCGTCACGATTGTGCAGAAAGTTTTCCCGAATACAAAA
TTGAAGTTCCACAAGAGACATTTAACCTCAGCGTCCCACGATCAGCTTGCCCGAGTTGCCATACCCAGATTCGCGCCATC
GATAACATTCCTGTTATCAGCTGGCTACTGCTAAAAGGCAAATGCCACAACTGCCAAAGCAAAATCAGTGCTCGCTACCC
TCTGATCGAGTTGCTCACGGGGGGCTTGTGCTTGCTGGTTGCCACCACCTTACCCGCCAGTTACTTCACTATCGCGCTGC
TGTTTTTTACCTTCGTTTTAGTCGCAGCGACGTTTATCGATCTTGATACCATGTTGCTGCCTGATAGCCTGACTCTACCA
CTAATGTGGAGCGGCATTGCCTTAGCCCTGTTTGGCATCAGTCCAGTGAGCTTGCAAAATTCGGTCATTGGCGCCATGGC
CGGCTATTTGTGCCTATGGAGTGTCTACTGGGCTTTCAAACTCATCACCAAAAAAGAAGGTATGGGTTACGGTGATTTTA
AATTGCTTGCCGCGCTAGGCGCCTGGCTTGGCTGGCAGTACCTGCCGATGATCATCTTAGTCTCATCTTTGGTGGGATTG
GTGTTTGGGCTTATTCAATTACGTCTACAGAGCAAAGGCATTGATAAAGCGTTTCCATTTGGTCCTTACTTAGCCATCGC
TGGTTGGATCGCAATGTTATGGGGGGAGCAGATCCTCCATGGCTACTACCGTTTTCTACTAGGAGCGTAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (135-244)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (17-122)

3D structure

• AlphaFold DB: A0A1C7F5Z7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio campbellii strain DS40M4	76.736	99.654	0.765
pilD	Vibrio cholerae strain A1552	69.792	99.654	0.696
pilD	Neisseria gonorrhoeae MS11	53.16	93.08	0.495
pilD	Acinetobacter nosocomialis M2	53.008	92.042	0.488
pilD	Acinetobacter baumannii D1279779	52.632	92.042	0.484