Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   BAY15_RS14990 Genome accession   NZ_CP016294
Coordinates   3367451..3367858 (-) Length   135 a.a.
NCBI ID   WP_068853815.1    Uniprot ID   A0AAP5AGY7
Organism   Stenotrophomonas rhizophila strain QL-P4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 3347303..3378064 3367451..3367858 within 0


Gene organization within MGE regions


Location: 3347303..3378064
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BAY15_RS14900 (BAY15_2919) - 3347303..3347851 (+) 549 WP_068853797.1 SymE family type I addiction module toxin -
  BAY15_RS14905 (BAY15_2920) - 3348043..3348744 (-) 702 WP_167693304.1 hypothetical protein -
  BAY15_RS18945 - 3349269..3349496 (-) 228 WP_083214200.1 hypothetical protein -
  BAY15_RS14910 (BAY15_2921) - 3349723..3350052 (-) 330 WP_157771754.1 hypothetical protein -
  BAY15_RS14915 (BAY15_2922) - 3350045..3350452 (-) 408 WP_068853800.1 hypothetical protein -
  BAY15_RS14920 (BAY15_2923) - 3350650..3351126 (-) 477 WP_157771755.1 hypothetical protein -
  BAY15_RS14925 - 3351518..3352000 (-) 483 WP_068853802.1 hypothetical protein -
  BAY15_RS14930 (BAY15_2924) - 3352209..3352571 (-) 363 WP_157771756.1 hypothetical protein -
  BAY15_RS19245 - 3353217..3353489 (-) 273 WP_157771757.1 hypothetical protein -
  BAY15_RS14935 - 3354453..3354749 (-) 297 WP_157771758.1 hypothetical protein -
  BAY15_RS19250 - 3354749..3354967 (-) 219 WP_157771759.1 hypothetical protein -
  BAY15_RS19255 - 3355351..3355707 (-) 357 WP_157771760.1 hypothetical protein -
  BAY15_RS14945 (BAY15_2926) - 3355819..3356037 (-) 219 WP_157771761.1 hypothetical protein -
  BAY15_RS14950 (BAY15_2927) - 3357011..3357385 (-) 375 WP_157771762.1 DUF6869 domain-containing protein -
  BAY15_RS14955 (BAY15_2928) - 3357844..3358548 (-) 705 WP_157771763.1 hypothetical protein -
  BAY15_RS14960 (BAY15_2929) - 3358642..3359421 (-) 780 WP_068853809.1 hypothetical protein -
  BAY15_RS19260 - 3359443..3359823 (-) 381 WP_157771764.1 hypothetical protein -
  BAY15_RS14965 (BAY15_2930) - 3360307..3360837 (-) 531 WP_157771765.1 hypothetical protein -
  BAY15_RS14975 (BAY15_2931) - 3361810..3364848 (-) 3039 WP_068853812.1 hypothetical protein -
  BAY15_RS14980 (BAY15_2932) pilB 3365132..3366865 (-) 1734 WP_068853813.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BAY15_RS14985 (BAY15_2933) pilA/pilAI 3366922..3367332 (-) 411 WP_068853814.1 pilin Machinery gene
  BAY15_RS14990 (BAY15_2934) pilA/pilAI 3367451..3367858 (-) 408 WP_068853815.1 pilin Machinery gene
  BAY15_RS14995 (BAY15_2935) pilC 3368215..3369471 (+) 1257 WP_068853816.1 type II secretion system F family protein Machinery gene
  BAY15_RS15000 (BAY15_2936) pilD 3369478..3370341 (+) 864 WP_068853817.1 A24 family peptidase Machinery gene
  BAY15_RS15005 (BAY15_2937) coaE 3370349..3370966 (+) 618 WP_068853818.1 dephospho-CoA kinase -
  BAY15_RS15010 - 3370963..3371304 (-) 342 WP_237334272.1 hypothetical protein -
  BAY15_RS15020 (BAY15_2938) - 3371773..3372258 (-) 486 WP_068853820.1 hypothetical protein -
  BAY15_RS15025 (BAY15_2939) - 3372458..3373825 (-) 1368 WP_068853821.1 HAMP domain-containing sensor histidine kinase -
  BAY15_RS15030 (BAY15_2940) - 3373788..3374465 (-) 678 WP_017356179.1 response regulator transcription factor -
  BAY15_RS15035 (BAY15_2941) - 3374474..3374947 (-) 474 WP_068853822.1 hypothetical protein -
  BAY15_RS15040 (BAY15_2942) rimK 3375180..3376085 (-) 906 WP_068853823.1 30S ribosomal protein S6--L-glutamate ligase -
  BAY15_RS15045 (BAY15_2943) - 3376343..3376729 (+) 387 WP_208856107.1 hypothetical protein -
  BAY15_RS15050 (BAY15_2944) - 3376733..3377704 (+) 972 WP_068853825.1 RES family NAD+ phosphorylase -
  BAY15_RS15055 (BAY15_2945) - 3377711..3378064 (-) 354 WP_068853826.1 nuclear transport factor 2 family protein -

Sequence

Protein


Download         Length: 135 a.a.        Molecular weight: 14048.19 Da        Isoelectric Point: 8.4781

>NTDB_id=186565 BAY15_RS14990 WP_068853815.1 3367451..3367858(-) (pilA/pilAI) [Stenotrophomonas rhizophila strain QL-P4]
MKKQQGFTLIELMIVVAIIAILAAIAIPAYQDYVVRSQGASALAEITPAKVGFEQAVNEGKTPSTAAADAGFVGVGPSTS
YCTVTVGADNIQCVTKGGNATKFNGKKVIWTRTADTGLWACTSDLDAKYKPGKCT

Nucleotide


Download         Length: 408 bp        

>NTDB_id=186565 BAY15_RS14990 WP_068853815.1 3367451..3367858(-) (pilA/pilAI) [Stenotrophomonas rhizophila strain QL-P4]
ATGAAGAAGCAGCAGGGCTTTACCCTCATCGAACTGATGATCGTTGTCGCGATCATCGCCATCCTGGCCGCCATTGCCAT
CCCGGCTTACCAGGACTACGTGGTCCGCTCACAGGGCGCCTCGGCTCTGGCCGAAATCACCCCGGCCAAGGTCGGTTTCG
AGCAGGCTGTCAACGAAGGCAAGACCCCGTCGACTGCCGCCGCTGACGCTGGCTTCGTTGGCGTGGGCCCGTCCACCTCG
TACTGCACCGTGACCGTCGGCGCTGACAACATCCAGTGCGTCACCAAGGGCGGCAACGCCACCAAGTTCAACGGCAAGAA
GGTCATTTGGACCCGTACCGCCGACACCGGTCTGTGGGCTTGCACCTCGGACCTGGACGCCAAGTACAAGCCGGGCAAGT
GCACCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-135)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

66.667

100

0.667
  pilA/pilAII Pseudomonas stutzeri DSM 10701

63.91

98.519

0.63
  pilA Pseudomonas aeruginosa PAK

47.059

100

0.533
  pilA Acinetobacter baumannii strain A118

48.252

100

0.511
  comP Acinetobacter baylyi ADP1

41.497

100

0.452
  pilA Ralstonia pseudosolanacearum GMI1000

37.342

100

0.437
  pilA2 Legionella pneumophila strain ERS1305867

41.606

100

0.422
  pilA2 Legionella pneumophila str. Paris

40.876

100

0.415
  pilA Vibrio parahaemolyticus RIMD 2210633

40.741

100

0.407
  pilA Vibrio cholerae strain A1552

38.732

100

0.407
  pilA Vibrio cholerae C6706

38.732

100

0.407
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

38.732

100

0.407
  pilE Neisseria gonorrhoeae strain FA1090

39.2

92.593

0.363

Multiple sequence alignment