Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BA022_RS11145 Genome accession   NZ_CP016278
Coordinates   2375272..2377005 (+) Length   577 a.a.
NCBI ID   WP_088887535.1    Uniprot ID   -
Organism   Diaphorobacter nitroreducens strain SL-205     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2370272..2382005
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BA022_RS11115 (BA022_10860) proB 2370762..2371904 (-) 1143 WP_047349967.1 glutamate 5-kinase -
  BA022_RS11120 (BA022_10865) cgtA 2371978..2373051 (-) 1074 WP_012655754.1 Obg family GTPase CgtA -
  BA022_RS11125 (BA022_10870) rpmA 2373198..2373455 (-) 258 WP_011804286.1 50S ribosomal protein L27 -
  BA022_RS11130 (BA022_10875) rplU 2373473..2373784 (-) 312 WP_012655753.1 50S ribosomal protein L21 -
  BA022_RS11135 (BA022_10880) - 2373954..2374937 (+) 984 WP_187305962.1 polyprenyl synthetase family protein -
  BA022_RS11145 (BA022_10890) pilB 2375272..2377005 (+) 1734 WP_088887535.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BA022_RS11150 (BA022_10895) pilC 2377026..2378243 (+) 1218 WP_088887536.1 type II secretion system F family protein Machinery gene
  BA022_RS11155 (BA022_10900) - 2378243..2379124 (+) 882 WP_088887537.1 prepilin peptidase -
  BA022_RS11160 (BA022_10905) coaE 2379156..2379749 (+) 594 WP_228403569.1 dephospho-CoA kinase -
  BA022_RS11165 (BA022_10910) zapD 2379840..2380595 (+) 756 WP_012655747.1 cell division protein ZapD -
  BA022_RS11170 (BA022_10915) - 2380602..2380832 (+) 231 WP_011804249.1 DNA gyrase inhibitor YacG -
  BA022_RS11175 (BA022_10920) - 2380901..2381347 (-) 447 WP_088887539.1 NUDIX domain-containing protein -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 63686.07 Da        Isoelectric Point: 5.3559

>NTDB_id=186496 BA022_RS11145 WP_088887535.1 2375272..2377005(+) (pilB) [Diaphorobacter nitroreducens strain SL-205]
MAAIDTQKEAQPIALPGLARALMSVGKLDQKAAEEIYKKSQNSRSSFIAELTESKIITPSDLAHTVSTVFGAPLLDVDAI
DRQRIPKDLLDVKLCQSYKVIALSKRNNRLIVATADPTFQEAAEKIKFTTQMGVDWIIAEYDKLLRLIEASTKSGTETLD
GINSNNDFEFGDIPVDETPDEDADNPAEVEDAPVVKFLHKMLLDAFNMRASDLHFEPYEHHYRVRFRIDGELREIASPPI
AIKEKLASRIKVISRLDISEKRVPQDGRMKLKVGPDRVIDFRVSTLPTLFGEKIVIRILDPSSAKLGIDALGYEPEEKER
LLQAINRPYGMILVTGPTGSGKTVSLYTCLNLLNKPGVNIATAEDPSEINLPGVNQVNVNEKAGLTFAVALKSFLRQDPD
IIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCPNCKAPVDIPH
ETLLEAGYRDEEIDGSWVTYRPVGCSACNNGYKGRVGIYQVMPVSEEIQRIILRDGSALEIAEQARLEGVRSLRESGLHK
AKMGLTSLEEVLAVTNE

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=186496 BA022_RS11145 WP_088887535.1 2375272..2377005(+) (pilB) [Diaphorobacter nitroreducens strain SL-205]
ATGGCTGCTATCGACACACAGAAAGAAGCTCAACCCATTGCCCTACCCGGACTGGCGCGCGCGTTGATGTCTGTGGGCAA
ATTGGATCAAAAAGCGGCGGAAGAGATTTACAAAAAGTCACAAAATAGCCGCAGTAGCTTTATTGCAGAGCTCACGGAAT
CGAAAATCATCACGCCAAGTGATCTGGCCCATACGGTATCCACCGTATTCGGTGCCCCCTTGCTAGATGTCGATGCGATC
GACCGGCAGCGCATCCCCAAAGATCTGTTGGACGTCAAACTGTGCCAGTCCTATAAAGTCATCGCTCTCAGTAAGCGCAA
TAACCGGCTCATAGTCGCCACCGCGGATCCGACATTTCAAGAGGCCGCAGAAAAGATCAAGTTCACCACACAAATGGGGG
TGGACTGGATCATCGCGGAATATGACAAACTGCTCCGCTTGATTGAAGCGAGCACGAAGAGCGGTACAGAAACGCTCGAC
GGCATTAACAGTAATAATGATTTTGAGTTCGGCGATATTCCCGTTGATGAAACTCCGGACGAGGACGCTGACAATCCCGC
GGAAGTCGAGGATGCTCCCGTTGTCAAATTTCTGCACAAGATGCTGCTGGATGCTTTCAATATGCGTGCATCCGATCTGC
ATTTTGAGCCCTATGAGCACCATTACCGTGTCCGCTTCCGCATAGATGGCGAATTGCGCGAAATCGCATCGCCGCCTATT
GCCATCAAGGAGAAGCTGGCCTCGCGCATCAAGGTGATCTCCCGCCTGGATATTTCGGAAAAACGCGTGCCCCAGGATGG
CCGCATGAAGCTCAAGGTTGGGCCGGACCGGGTTATCGATTTCCGCGTCAGCACCCTGCCTACGCTCTTTGGCGAGAAGA
TCGTGATCCGTATTCTCGATCCGAGCAGCGCCAAGCTGGGTATTGACGCACTGGGATATGAGCCCGAGGAAAAGGAGCGT
TTGCTGCAGGCCATCAACCGCCCTTATGGAATGATCCTGGTGACCGGACCCACGGGCTCCGGGAAGACCGTGTCGCTCTA
CACATGTCTCAATTTGTTGAACAAGCCCGGCGTCAATATTGCGACCGCTGAGGATCCCTCGGAAATCAACCTCCCGGGTG
TGAACCAAGTCAACGTCAACGAAAAAGCCGGCCTCACGTTTGCCGTCGCGCTCAAGTCATTCCTTCGCCAAGATCCGGAC
ATCATCATGGTGGGTGAAATCCGGGATCTGGAAACCGCAGACATTTCGATCAAAGCCGCGCAGACGGGTCACTTGGTGCT
GTCCACCCTGCACACCAATGACGCCCCCACGACATTGACGCGCATGCGCAATATGGGCATCGCGCCCTTCAATATCGCAT
CCAGCGTGATTCTTATTACGGCGCAGCGACTGGCGCGTCGCTTGTGCCCCAATTGCAAGGCGCCCGTGGACATTCCGCAT
GAGACGCTGCTGGAGGCCGGCTACCGGGACGAGGAGATCGACGGCTCATGGGTTACCTACCGACCGGTGGGCTGCTCCGC
CTGCAATAACGGATACAAGGGGCGCGTGGGCATTTACCAGGTCATGCCGGTGTCCGAAGAAATCCAGCGCATCATCCTGC
GCGATGGAAGCGCGCTGGAAATTGCCGAACAGGCACGCCTGGAGGGCGTCCGTTCTCTGCGTGAATCCGGGCTTCACAAG
GCAAAAATGGGATTGACCTCCTTGGAAGAGGTCTTGGCTGTCACCAACGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(51-150)

Type II/IV secretion system protein (T2SSE)

(200-467)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.355

99.48

0.541
  pilB Acinetobacter baumannii D1279779

53.901

97.747

0.527
  pilF Neisseria gonorrhoeae MS11

52.389

97.92

0.513
  pilB Legionella pneumophila strain ERS1305867

49.735

97.92

0.487
  pilB Vibrio parahaemolyticus RIMD 2210633

45.552

97.4

0.444
  pilB Vibrio campbellii strain DS40M4

45.455

97.227

0.442
  pilB Vibrio cholerae strain A1552

46.981

91.854

0.432
  pilF Thermus thermophilus HB27

39.897

100

0.404
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.121

92.721

0.381

Multiple sequence alignment