Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   A6B44_RS08125 Genome accession   NZ_CP016180
Coordinates   1710253..1711317 (-) Length   354 a.a.
NCBI ID   WP_090920215.1    Uniprot ID   A0A1H7UWI6
Organism   Pasteurella skyensis strain 95A1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1705253..1716317
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A6B44_RS08095 (A6B44_08025) - 1706314..1706934 (-) 621 WP_090920199.1 LysE family transporter -
  A6B44_RS08100 - 1707120..1707263 (+) 144 WP_176673498.1 hypothetical protein -
  A6B44_RS08105 (A6B44_08030) - 1707287..1708201 (+) 915 WP_090920202.1 YiiG family protein -
  A6B44_RS08110 (A6B44_08035) - 1708264..1708746 (-) 483 WP_090920205.1 phosphatidylglycerophosphatase A -
  A6B44_RS08115 (A6B44_08040) thiL 1708766..1709719 (-) 954 WP_090920208.1 thiamine-phosphate kinase -
  A6B44_RS08120 (A6B44_08045) nusB 1709732..1710145 (-) 414 WP_090920212.1 transcription antitermination factor NusB -
  A6B44_RS08125 (A6B44_08050) comE 1710253..1711317 (-) 1065 WP_090920215.1 type IV pilus secretin PilQ Machinery gene
  A6B44_RS08130 (A6B44_08055) - 1711319..1711828 (-) 510 WP_090920218.1 hypothetical protein -
  A6B44_RS08135 (A6B44_08060) - 1711828..1712343 (-) 516 WP_090920221.1 hypothetical protein -
  A6B44_RS08140 (A6B44_08065) - 1712319..1713068 (-) 750 WP_090920224.1 hypothetical protein -
  A6B44_RS08145 (A6B44_08070) - 1713176..1715749 (+) 2574 WP_090920227.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 39255.89 Da        Isoelectric Point: 6.1281

>NTDB_id=186111 A6B44_RS08125 WP_090920215.1 1710253..1711317(-) (comE) [Pasteurella skyensis strain 95A1]
MKKIVLLLFGFYSTCLVADPFYKDVTSNETVRIDEENDQHIIPINEDLPIKNIHLNYARAVDIVETLTKGHGSLLDGGYI
HFDKQTNTLILKGDKSLLAKLTTLIHQLDKPIKQVAIEARIVTISSENLQELGVRWGMFSPTESASKFAGRLEGNGFSTN
NLNVNFPVLGNAASAVVQIASFSNRLLDLELTALEQENSIEIIASPSLITTNEHKASIKQGTEIAYPQHNEKGDITDVEF
RDAVLGLEVIPHFSKNNQILLDLLVTQNSPNTTGASSQQWVTIDKQEIKTQVLARHGETIVLGGIFQNSISKEKEGVPIL
GSLPFLKYLFSHTKDKVVKRELVIFVTPHIINPQ

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=186111 A6B44_RS08125 WP_090920215.1 1710253..1711317(-) (comE) [Pasteurella skyensis strain 95A1]
ATGAAAAAAATAGTGCTGTTGTTATTTGGGTTTTATAGTACTTGTCTTGTGGCAGATCCTTTTTACAAGGATGTGACATC
AAATGAAACAGTGCGAATAGATGAAGAAAATGATCAACATATAATACCTATAAACGAAGATCTACCGATTAAAAATATTC
ATTTAAACTATGCACGAGCTGTGGATATAGTAGAAACCTTAACAAAAGGGCATGGTTCGTTATTAGATGGAGGTTATATC
CATTTTGATAAACAAACAAATACGCTTATTTTAAAAGGTGATAAAAGTTTACTTGCTAAATTGACAACTCTCATTCATCA
ATTAGATAAACCAATCAAACAAGTAGCGATAGAAGCAAGGATTGTGACAATTAGCAGTGAAAATTTACAAGAATTAGGTG
TTCGATGGGGAATGTTTAGTCCTACAGAAAGTGCGAGTAAATTTGCAGGGCGTTTAGAGGGGAATGGATTTTCAACAAAT
AATTTGAATGTTAATTTCCCCGTTCTTGGAAATGCAGCATCTGCAGTTGTGCAAATTGCCAGCTTTAGTAATCGTCTTTT
AGATTTAGAACTCACTGCATTAGAGCAAGAAAATAGCATTGAAATTATTGCTAGTCCTAGTTTAATTACAACGAATGAAC
ATAAAGCGAGTATCAAACAGGGAACAGAAATTGCCTATCCTCAACATAATGAAAAAGGGGATATAACAGATGTTGAATTT
CGAGATGCGGTCTTAGGTTTGGAAGTGATTCCTCATTTCTCTAAAAATAATCAAATTTTGTTAGATTTACTAGTGACTCA
AAATTCGCCGAATACCACTGGTGCAAGTAGTCAGCAATGGGTAACTATTGATAAACAAGAGATTAAAACACAAGTGTTGG
CTCGTCATGGTGAAACTATTGTTTTAGGTGGAATTTTTCAAAATAGTATTTCTAAAGAAAAAGAAGGCGTGCCTATTTTA
GGTAGTTTACCGTTTTTAAAGTATTTATTTAGTCATACCAAAGATAAGGTAGTTAAACGAGAGTTAGTGATTTTTGTGAC
ACCACATATAATTAACCCTCAGTAA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(193-351)

Bacterial type II/III secretion system short domain (Secretin_N)

(52-114)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1H7UWI6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Glaesserella parasuis strain SC1401

61.967

86.158

0.534
  comE Haemophilus influenzae Rd KW20

55.263

85.876

0.475
  comE Haemophilus influenzae 86-028NP

53.947

85.876

0.463
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

43.789

90.96

0.398
  pilQ Vibrio cholerae strain A1552

43.789

90.96

0.398
  pilQ Vibrio campbellii strain DS40M4

42.724

91.243

0.39
  pilQ Legionella pneumophila strain ERS1305867

39.091

93.22

0.364
  pilQ Legionella pneumophila strain Lp02

39.091

93.22

0.364

Multiple sequence alignment