Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   A6B44_RS03345 Genome accession   NZ_CP016180
Coordinates   721644..723041 (+) Length   465 a.a.
NCBI ID   WP_090922473.1    Uniprot ID   A0A1H7YFZ6
Organism   Pasteurella skyensis strain 95A1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 716644..728041
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A6B44_RS03320 (A6B44_03285) ndk 716786..717202 (+) 417 WP_090922481.1 nucleoside-diphosphate kinase -
  A6B44_RS03325 (A6B44_03290) - 717225..718781 (-) 1557 WP_176673455.1 peptide ABC transporter substrate-binding protein -
  A6B44_RS03330 (A6B44_03295) - 718793..719443 (-) 651 WP_090922477.1 YchE family NAAT transporter -
  A6B44_RS03335 (A6B44_03300) radA 719614..720990 (-) 1377 WP_090922475.1 DNA repair protein RadA -
  A6B44_RS03340 (A6B44_03305) pilA 721180..721617 (+) 438 WP_090922485.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  A6B44_RS03345 (A6B44_03310) pilB 721644..723041 (+) 1398 WP_090922473.1 GspE/PulE family protein Machinery gene
  A6B44_RS03350 (A6B44_03315) pilC 723028..724218 (+) 1191 WP_090922471.1 type II secretion system F family protein Machinery gene
  A6B44_RS03355 (A6B44_03320) - 724215..724892 (+) 678 WP_090922469.1 prepilin peptidase -
  A6B44_RS03360 (A6B44_03325) coaE 724916..725536 (+) 621 WP_090922467.1 dephospho-CoA kinase -
  A6B44_RS03365 (A6B44_03330) yacG 725537..725728 (+) 192 WP_090922466.1 DNA gyrase inhibitor YacG -
  A6B44_RS03370 (A6B44_03335) tnaA 726174..727592 (+) 1419 WP_090922465.1 tryptophanase -

Sequence

Protein


Download         Length: 465 a.a.        Molecular weight: 53103.72 Da        Isoelectric Point: 5.6283

>NTDB_id=186105 A6B44_RS03345 WP_090922473.1 721644..723041(+) (pilB) [Pasteurella skyensis strain 95A1]
MQYNVYDLMNQKVVKLSQDRWQQNIKDKPLLLRYLALPIKEDEKTLWLAIDDENNLNVCEIFSFLTHKEIEPVLVSSDEL
KYLLNELSPSYVDNQENESQITLYNQHNSEESIDNSDPIIQLLETIFKYGLQKNASDIHIEPHKEILVVRLRIDGVLQVY
KNISLNIANRVISRVKLLAKLDISETRLPQDGQFYFQTQLNERLDFRVSTLPTHYGEKLVLRLQKHNPTQLDFVSLGFNP
SQRDILVNALHKPQGLILVTGPTGSGKSLTLYSALNYLNNTDKHILTAEDPIEIIIDGVMQSQVNRSIGIDFSQLLRTFL
RQDPDIIMLGEIRDQESAEIALRAAQTGHLVLATLHTNDAPSTIERLLQLGIKEYEIKHSLLLVITQRLVRKLCALCSGE
SCEHCFDGYNGRMGVFQCLTKNAKTFEKDTAYLDFATLFESAKQLIANKQTNQAEIQRVIGNESV

Nucleotide


Download         Length: 1398 bp        

>NTDB_id=186105 A6B44_RS03345 WP_090922473.1 721644..723041(+) (pilB) [Pasteurella skyensis strain 95A1]
ATGCAATATAATGTTTATGATTTAATGAATCAAAAAGTCGTAAAGCTATCTCAGGATAGGTGGCAGCAAAATATTAAGGA
TAAACCACTTTTATTGCGTTATTTAGCATTACCTATCAAAGAAGATGAGAAAACACTTTGGCTTGCCATTGATGATGAGA
ATAATCTAAATGTCTGTGAAATATTTTCATTTTTAACCCATAAAGAAATTGAACCCGTTTTGGTGTCTTCTGATGAGTTA
AAATATTTGTTAAATGAACTGTCTCCAAGTTATGTTGATAACCAAGAAAATGAATCGCAAATTACGCTTTATAATCAGCA
TAATAGTGAAGAAAGTATTGATAATTCTGATCCCATTATTCAATTATTAGAAACTATTTTTAAATATGGATTACAAAAAA
ATGCCTCTGATATCCATATTGAGCCGCATAAAGAAATATTAGTGGTTCGATTACGAATTGATGGGGTTTTGCAAGTATAT
AAAAATATTTCATTAAACATTGCGAATCGTGTTATTTCAAGGGTTAAACTACTGGCTAAATTGGATATTAGTGAAACCCG
TTTGCCTCAAGATGGGCAGTTTTATTTTCAAACACAGCTTAATGAACGGTTAGATTTTCGAGTGTCTACACTGCCCACTC
ATTATGGTGAAAAATTGGTATTACGATTACAAAAACATAATCCGACTCAGTTAGATTTTGTATCATTGGGTTTTAATCCT
TCTCAAAGGGATATATTGGTCAATGCACTACACAAACCGCAAGGGCTTATTTTAGTAACAGGTCCTACTGGTAGTGGCAA
AAGCCTTACGTTATATAGTGCATTGAATTATTTAAACAATACCGATAAACATATTTTAACCGCAGAAGATCCCATAGAAA
TTATTATTGATGGTGTAATGCAATCACAGGTGAATCGAAGTATCGGTATTGATTTTAGCCAATTATTACGCACTTTTTTA
CGTCAAGATCCAGATATTATTATGTTAGGTGAAATTCGAGATCAAGAGAGTGCTGAAATTGCATTACGAGCAGCACAAAC
AGGGCATTTAGTGTTGGCAACATTACATACGAATGATGCACCGTCAACCATAGAGCGTTTATTACAATTAGGTATTAAAG
AGTATGAAATTAAACATTCATTATTATTGGTGATTACACAGCGATTAGTACGAAAGCTGTGTGCGCTATGTAGTGGTGAA
AGTTGTGAACATTGCTTTGATGGTTATAACGGTAGAATGGGAGTGTTTCAATGTTTAACAAAAAATGCAAAAACTTTCGA
GAAAGATACCGCTTATTTAGATTTTGCCACTTTATTTGAGAGTGCAAAACAGCTTATTGCAAATAAGCAGACGAATCAAG
CAGAAATTCAGCGAGTGATTGGCAATGAATCTGTATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(130-392)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(29-89)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1H7YFZ6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

66.017

99.355

0.656
  pilB Haemophilus influenzae Rd KW20

55.385

97.849

0.542
  pilB Haemophilus influenzae 86-028NP

54.585

98.495

0.538
  pilB Vibrio cholerae strain A1552

38.193

100

0.4
  pilB Vibrio campbellii strain DS40M4

38.793

99.785

0.387
  pilB Vibrio parahaemolyticus RIMD 2210633

38.661

99.57

0.385
  pilB Acinetobacter baumannii D1279779

36.008

100

0.376
  pilB Acinetobacter baylyi ADP1

35.685

100

0.37
  pilB Legionella pneumophila strain ERS1305867

38.902

93.978

0.366

Multiple sequence alignment