Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   A6B44_RS03340 Genome accession   NZ_CP016180
Coordinates   721180..721617 (+) Length   145 a.a.
NCBI ID   WP_090922485.1    Uniprot ID   A0AAJ6N9T8
Organism   Pasteurella skyensis strain 95A1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 716180..726617
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A6B44_RS03320 (A6B44_03285) ndk 716786..717202 (+) 417 WP_090922481.1 nucleoside-diphosphate kinase -
  A6B44_RS03325 (A6B44_03290) - 717225..718781 (-) 1557 WP_176673455.1 peptide ABC transporter substrate-binding protein -
  A6B44_RS03330 (A6B44_03295) - 718793..719443 (-) 651 WP_090922477.1 YchE family NAAT transporter -
  A6B44_RS03335 (A6B44_03300) radA 719614..720990 (-) 1377 WP_090922475.1 DNA repair protein RadA -
  A6B44_RS03340 (A6B44_03305) pilA 721180..721617 (+) 438 WP_090922485.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  A6B44_RS03345 (A6B44_03310) pilB 721644..723041 (+) 1398 WP_090922473.1 GspE/PulE family protein Machinery gene
  A6B44_RS03350 (A6B44_03315) pilC 723028..724218 (+) 1191 WP_090922471.1 type II secretion system F family protein Machinery gene
  A6B44_RS03355 (A6B44_03320) - 724215..724892 (+) 678 WP_090922469.1 prepilin peptidase -
  A6B44_RS03360 (A6B44_03325) coaE 724916..725536 (+) 621 WP_090922467.1 dephospho-CoA kinase -
  A6B44_RS03365 (A6B44_03330) yacG 725537..725728 (+) 192 WP_090922466.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 15523.74 Da        Isoelectric Point: 5.0332

>NTDB_id=186104 A6B44_RS03340 WP_090922485.1 721180..721617(+) (pilA) [Pasteurella skyensis strain 95A1]
MQKSYLRLSQAFTLIELMIVIAIVAILAVVAMPSYTSYTQKAVLSEILTASAPYKSDVEICIYNMGEATNCNGGSNGIQA
DKSSSDTKYLKSIATSKGIITVEGKGTLKDYSYTLTPTYANNSIVWTATCAGEDTGIFPANFCSN

Nucleotide


Download         Length: 438 bp        

>NTDB_id=186104 A6B44_RS03340 WP_090922485.1 721180..721617(+) (pilA) [Pasteurella skyensis strain 95A1]
ATGCAAAAAAGTTATTTAAGACTCTCCCAGGCCTTCACTTTAATTGAACTGATGATTGTGATTGCAATCGTTGCCATTCT
TGCTGTCGTTGCTATGCCATCCTACACCAGTTATACGCAAAAAGCGGTACTGTCCGAAATTTTAACGGCTTCAGCACCCT
ATAAATCTGATGTGGAAATTTGTATTTATAATATGGGAGAGGCAACAAACTGTAATGGAGGCTCCAACGGTATTCAAGCA
GATAAATCATCAAGTGATACTAAGTACCTGAAATCTATTGCAACCAGTAAAGGGATCATTACCGTTGAAGGCAAAGGAAC
ATTGAAAGACTATAGTTATACCTTAACACCTACTTATGCAAATAATAGTATTGTTTGGACAGCAACTTGTGCAGGTGAAG
ATACAGGTATTTTTCCTGCTAACTTTTGTTCAAATTAG

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(9-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

64.029

95.862

0.614
  pilA Haemophilus influenzae 86-028NP

57.664

94.483

0.545
  pilA Haemophilus influenzae Rd KW20

54.745

94.483

0.517
  comP Acinetobacter baylyi ADP1

41.353

91.724

0.379

Multiple sequence alignment