Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   THL1_RS26300 Genome accession   NZ_CP015992
Coordinates   5749513..5751615 (-) Length   700 a.a.
NCBI ID   WP_069085991.1    Uniprot ID   -
Organism   Pseudomonas sp. TCU-HL1 isolate Hualien Taiwan     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5744513..5756615
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  THL1_RS26285 (THL1_5297) - 5746165..5747823 (-) 1659 WP_069085989.1 AAA family ATPase -
  THL1_RS26290 (THL1_5298) aroB 5747833..5748936 (-) 1104 WP_069085990.1 3-dehydroquinate synthase -
  THL1_RS26295 (THL1_5299) aroK 5748989..5749507 (-) 519 WP_083246014.1 shikimate kinase AroK -
  THL1_RS26300 (THL1_5300) pilQ 5749513..5751615 (-) 2103 WP_069085991.1 type IV pilus secretin PilQ Machinery gene
  THL1_RS26305 (THL1_5301) pilP 5751670..5752203 (-) 534 WP_069085992.1 type 4a pilus biogenesis lipoprotein PilP -
  THL1_RS26310 (THL1_5302) pilO 5752200..5752823 (-) 624 WP_069085993.1 type 4a pilus biogenesis protein PilO -
  THL1_RS26315 (THL1_5303) pilN 5752820..5753434 (-) 615 WP_069085994.1 type 4a pilus biogenesis protein PilN -
  THL1_RS26320 (THL1_5304) pilM 5753434..5754498 (-) 1065 WP_069085995.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 700 a.a.        Molecular weight: 74991.10 Da        Isoelectric Point: 4.9840

>NTDB_id=184985 THL1_RS26300 WP_069085991.1 5749513..5751615(-) (pilQ) [Pseudomonas sp. TCU-HL1 isolate Hualien Taiwan]
MKSSLSRLGITLLAALLSPALLAANLQALDVAALPGDRVELKLAFDEPVAAPRGYTIEQPARIALDLPGVSNKLGTKNRE
LGVGNARSVTVVEAKDRTRLIINLSTLAPYSTRVDGRNLYVLVGDAGSAPAASRAANAAPAPTAAPLAASQPVKSYARAG
RAIENIDFQRGDQGEGNVVITLSDPSVSPDIQEQGGKIRLDFSKTQLPEPLRVRLDVKDFATPVQFVSASGSGNNASISI
EPTGFYDYLAYQTDNKLTVSIKPLTQDDVEKRKAERFAYSGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVQGNITL
RLQNVPWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIAELAPLRRELIQVNYAKAADIAKLFQSVT
SGDPSAAGTDERGSITVDDRTNSIIAYQTQDKLDELRRIVTQLDIPVRQVMIEARIVEANVDYDKALGVNWRGASIGDNN
FVIGGGGTGRPAANQAGNVNLASAPFVDLGATGATSSIGIGFITDNTILDLELSAMEKTGNGEIVSQPKVVTSDKETAKI
LKGAEVPYQEASSSGATSTSFKEAALSLEVTPQITPDNRIIMEVKVTKDEPDFANALNGVPPIKKNEVNAKVLVSDGETI
VIGGVFSNTQTKAVDKVPFLGDLPFLGRMFRRDVVQDKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2103 bp        

>NTDB_id=184985 THL1_RS26300 WP_069085991.1 5749513..5751615(-) (pilQ) [Pseudomonas sp. TCU-HL1 isolate Hualien Taiwan]
ATGAAAAGCTCGCTATCGCGCCTAGGTATCACGCTATTGGCCGCCCTGCTGTCGCCAGCGCTGCTGGCTGCGAACCTGCA
GGCACTGGATGTTGCGGCCCTGCCGGGCGACAGGGTTGAACTCAAGCTGGCATTCGACGAGCCGGTGGCCGCTCCGCGCG
GCTACACCATCGAGCAGCCCGCTCGTATTGCCCTGGACCTGCCGGGTGTATCCAACAAGCTGGGTACGAAGAACCGCGAG
CTGGGCGTGGGCAATGCCCGCAGCGTGACCGTGGTCGAAGCCAAGGATCGCACCCGCCTGATCATCAACCTGTCTACCCT
GGCGCCTTACAGCACCCGTGTGGATGGTCGAAATCTCTACGTGCTGGTGGGTGATGCCGGTTCCGCACCGGCCGCGTCCC
GCGCCGCCAACGCAGCCCCGGCGCCCACGGCTGCTCCGCTGGCCGCATCGCAGCCGGTCAAGAGCTACGCTCGTGCCGGC
CGCGCAATCGAGAACATCGATTTCCAGCGTGGCGACCAGGGTGAAGGCAATGTGGTCATTACGCTGTCCGACCCTAGCGT
CAGCCCGGACATTCAGGAACAAGGTGGCAAGATTCGCCTGGACTTCTCCAAGACCCAGCTCCCTGAGCCCCTGCGCGTGC
GTCTGGACGTCAAGGACTTCGCCACACCGGTGCAGTTCGTCAGCGCGTCCGGCTCTGGCAACAATGCCAGCATTAGCATC
GAGCCAACCGGTTTCTATGATTACCTGGCGTACCAGACCGACAACAAACTGACCGTCAGCATCAAGCCGCTGACCCAGGA
TGATGTTGAAAAGCGCAAGGCTGAGCGTTTCGCCTACTCCGGCGAGAAGCTGTCGCTGAACTTCCAGGACATCGACGTGC
GTTCGGTGTTGCAGCTGATTGCCGACTTCACGGACCTGAACCTGGTGGCTTCGGACACTGTGCAGGGCAACATCACCCTG
CGCCTGCAGAATGTGCCCTGGGATCAGGCGCTGGATCTGGTGCTGAAGACCAAGGGTCTGGACAAGCGCAAGGTCGGTAA
CGTGCTGCTGGTTGCGCCGGCTGACGAGATCGCCGCGCGCGAGCGGCAGGAGCTGGAATCGCAGAAGCAGATCGCCGAGC
TGGCGCCCCTGCGTCGCGAGCTGATCCAGGTGAACTACGCCAAGGCTGCGGATATTGCCAAGCTGTTCCAGTCCGTCACC
AGCGGTGATCCGAGTGCTGCCGGCACTGACGAGCGCGGCTCCATCACGGTGGATGACCGTACCAACAGCATCATTGCCTA
TCAGACCCAGGACAAGCTGGACGAGCTGCGTCGTATCGTCACCCAACTGGATATTCCGGTTCGCCAGGTGATGATCGAAG
CACGCATCGTCGAAGCCAACGTCGATTATGACAAGGCCCTGGGCGTCAACTGGCGCGGCGCGAGCATCGGCGACAACAAC
TTCGTCATCGGCGGCGGTGGTACTGGTCGTCCGGCTGCTAACCAGGCCGGCAACGTGAACCTCGCCAGCGCGCCCTTCGT
CGATCTGGGTGCCACGGGGGCCACCTCCAGCATCGGTATCGGTTTCATCACCGATAACACCATTCTCGACCTCGAACTGT
CGGCGATGGAGAAGACTGGCAATGGCGAGATCGTGTCGCAGCCGAAGGTGGTTACCTCGGACAAGGAAACCGCCAAGATC
CTGAAGGGTGCCGAAGTGCCTTACCAGGAAGCCAGCTCCAGCGGCGCCACCAGCACCTCCTTCAAGGAAGCGGCCCTGTC
CCTGGAAGTGACTCCGCAGATCACGCCGGACAACCGCATCATCATGGAGGTCAAGGTCACCAAGGATGAGCCGGACTTCG
CCAACGCGCTGAACGGTGTACCGCCGATCAAGAAAAACGAGGTCAATGCCAAGGTACTGGTCAGCGATGGTGAAACCATT
GTCATAGGTGGGGTATTCTCCAACACCCAAACCAAGGCGGTGGACAAGGTGCCCTTCCTCGGCGACCTGCCCTTCCTCGG
CCGCATGTTCCGTCGCGACGTGGTTCAGGACAAGAAATCCGAGCTCCTGGTATTCCTCACTCCCCGCATCATGAACAACC
AGGCCATTGCGGTGAGTCGCTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(381-449)

Bacterial type II and III secretion system protein (Secretin)

(535-691)

Secretin and TonB N terminus short domain (STN)

(307-353)

AMIN domain (AMIN)

(38-120)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

77.406

100

0.793
  comQ Acinetobacter baylyi ADP1

43.228

99.143

0.429
  pilQ Acinetobacter baumannii strain A118

41.076

100

0.414
  pilQ Acinetobacter baumannii D1279779

41.076

100

0.414
  pilQ Legionella pneumophila strain Lp02

38.382

97.143

0.373
  pilQ Legionella pneumophila strain ERS1305867

38.235

97.143

0.371

Multiple sequence alignment