Detailed information NTG0184986

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: THL1_RS26320
• Genome accession: NZ_CP015992
• Coordinates: 5753434..5754498 (-)
• Length: 354 a.a.
• NCBI ID: WP_069085995.1
• Uniprot ID: A0A1B3EE92
• Organism: Pseudomonas sp. TCU-HL1 isolate Hualien Taiwan
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5748434..5759498

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
THL1_RS26295 (THL1_5299)	aroK	5748989..5749507 (-)	519	WP_083246014.1	shikimate kinase AroK	-
THL1_RS26300 (THL1_5300)	pilQ	5749513..5751615 (-)	2103	WP_069085991.1	type IV pilus secretin PilQ	Machinery gene
THL1_RS26305 (THL1_5301)	pilP	5751670..5752203 (-)	534	WP_069085992.1	type 4a pilus biogenesis lipoprotein PilP	-
THL1_RS26310 (THL1_5302)	pilO	5752200..5752823 (-)	624	WP_069085993.1	type 4a pilus biogenesis protein PilO	-
THL1_RS26315 (THL1_5303)	pilN	5752820..5753434 (-)	615	WP_069085994.1	type 4a pilus biogenesis protein PilN	-
THL1_RS26320 (THL1_5304)	pilM	5753434..5754498 (-)	1065	WP_069085995.1	pilus assembly protein PilM	Machinery gene
THL1_RS26325 (THL1_5305)	-	5754683..5757118 (+)	2436	WP_162493739.1	penicillin-binding protein 1A	-
THL1_RS26330 (THL1_5306)	-	5757229..5758040 (+)	812	Protein_5311	transposase	-
THL1_RS26335 (THL1_5308)	-	5758167..5759435 (-)	1269	WP_069085997.1	malic enzyme-like NAD(P)-binding protein	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 37779.01 Da
• Isoelectric Point: 4.4526

>NTDB_id=184986 THL1_RS26320 WP_069085995.1 5753434..5754498(-) (pilM) [Pseudomonas sp. TCU-HL1 isolate Hualien Taiwan]
MLGLFNKKANSLLGIDISSTSVKLLELSRSGSRFKVESYAVEPLPPNAVVEKNIAELEGVGQALARVVTKARTGVKAAAV
AVAGSAVITKTIEMEAGLSDDDLENQLKIEADQYIPYPLEEVAIDFEVQGASARNSERVDVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERAYSLLSAQLGEGHDELTVAVVDVGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLQPFKDAVVQQVSRSLQFFFAAGQFNDVDYILLAGGTASIPDLDRLIQQKIGTPTLVANP
FADMALSSKVNAGALASDAPALMIACGLAMRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=184986 THL1_RS26320 WP_069085995.1 5753434..5754498(-) (pilM) [Pseudomonas sp. TCU-HL1 isolate Hualien Taiwan]
GTGCTAGGGCTCTTCAATAAGAAAGCGAATTCGCTGTTGGGGATCGACATCAGCTCGACCTCAGTCAAGCTCCTTGAATT
GAGTCGCTCGGGAAGCCGCTTCAAAGTGGAGTCCTACGCCGTCGAGCCGCTCCCGCCAAACGCGGTGGTCGAAAAGAACA
TCGCGGAGCTGGAGGGCGTCGGCCAGGCGCTTGCTCGCGTGGTCACCAAGGCGCGTACCGGGGTCAAGGCCGCTGCAGTG
GCCGTGGCGGGTTCGGCTGTGATCACCAAGACCATCGAGATGGAAGCCGGGCTCTCCGATGATGACCTGGAGAACCAGCT
GAAGATCGAGGCTGACCAGTACATCCCCTATCCGCTGGAAGAAGTGGCCATCGACTTCGAGGTGCAAGGCGCCTCTGCGC
GCAACTCGGAACGCGTCGACGTGCTGCTGGCCGCTTGCCGAAAGGAAAACGTGGAAGTTCGCGAGGCCGCACTGGCCCTG
GCCGGACTCACTGCGAAGGTGGTCGATGTCGAGGCTTACGCGCTTGAACGGGCCTACTCGCTGCTCTCCGCCCAGCTTGG
CGAGGGCCATGATGAGCTGACGGTCGCGGTCGTCGATGTCGGTGCCACCATGACAACCCTCAGCGTGCTGCACAATGGCC
GCACCATTTATACCCGCGAACAGCTCTTCGGCGGTCGCCAGTTGACCGAGGAGATTCAGCGCCGCTACGGCCTCTCGGTC
GAAGAGGCCGGTCTTGCCAAGAAGCAGGGCGGTCTTCCGGACGACTACGACAGCGAGGTTCTGCAACCGTTCAAGGATGC
CGTTGTTCAGCAGGTTTCCCGTTCGCTGCAGTTCTTCTTCGCTGCAGGGCAGTTCAACGATGTGGACTACATTCTCCTGG
CGGGTGGTACTGCGTCCATCCCTGATCTGGATCGCCTGATCCAGCAGAAGATCGGCACCCCGACCCTGGTTGCCAACCCC
TTCGCCGACATGGCACTGAGTAGCAAGGTCAATGCTGGCGCACTGGCCAGCGATGCACCGGCGCTGATGATTGCTTGCGG
CTTGGCGATGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: A0A1B3EE92

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	56.497	100	0.565
comM	Acinetobacter nosocomialis M2	56.497	100	0.565
comM	Acinetobacter baylyi ADP1	52.825	100	0.528
pilM	Legionella pneumophila strain ERS1305867	46.61	100	0.466