Detailed information    

insolico Bioinformatically predicted

Overview


Name   comE1/comEA   Type   Machinery gene
Locus tag   THL1_RS09870 Genome accession   NZ_CP015992
Coordinates   2140605..2140925 (+) Length   106 a.a.
NCBI ID   WP_069083111.1    Uniprot ID   A0A1B3E4S9
Organism   Pseudomonas sp. TCU-HL1 isolate Hualien Taiwan     
Function   dsDNA binding (predicted from homology)   
DNA binding and uptake

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 2121925..2140925 2140605..2140925 within 0
IScluster/Tn 2141039..2144923 2140605..2140925 flank 114


Gene organization within MGE regions


Location: 2121925..2144923
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  THL1_RS09795 (THL1_1974) ihfB 2121925..2122209 (+) 285 WP_069083097.1 integration host factor subunit beta -
  THL1_RS09800 (THL1_1975) - 2123052..2125925 (+) 2874 WP_083245856.1 lipid II flippase MurJ -
  THL1_RS09805 (THL1_1976) wbpA 2126223..2127536 (+) 1314 WP_069083099.1 UDP-N-acetyl-D-glucosamine 6-dehydrogenase -
  THL1_RS09810 (THL1_1977) wbpB 2127555..2128505 (+) 951 WP_069083100.1 UDP-N-acetyl-2-amino-2-deoxy-D-glucuronate oxidase -
  THL1_RS09815 (THL1_1978) - 2128513..2129094 (+) 582 WP_069083101.1 acyltransferase -
  THL1_RS09820 (THL1_1979) - 2129098..2130195 (+) 1098 WP_069083102.1 DegT/DnrJ/EryC1/StrS family aminotransferase -
  THL1_RS09825 (THL1_1980) - 2130298..2131332 (+) 1035 WP_069083103.1 hypothetical protein -
  THL1_RS09830 (THL1_1981) - 2131332..2132732 (+) 1401 WP_069083104.1 lipopolysaccharide biosynthesis protein -
  THL1_RS09835 (THL1_1982) - 2133269..2133874 (+) 606 WP_069083105.1 glycosyltransferase -
  THL1_RS09840 (THL1_1983) - 2133926..2134816 (+) 891 WP_069083106.1 glycosyltransferase family 2 protein -
  THL1_RS09845 (THL1_1984) - 2134871..2136007 (+) 1137 WP_069083107.1 glycosyltransferase -
  THL1_RS09850 (THL1_1985) - 2136020..2136616 (+) 597 WP_069083108.1 sugar transferase -
  THL1_RS09855 (THL1_1986) - 2136613..2137230 (+) 618 WP_069083109.1 acetyltransferase -
  THL1_RS09860 (THL1_1987) - 2137295..2138470 (+) 1176 WP_069083110.1 DegT/DnrJ/EryC1/StrS family aminotransferase -
  THL1_RS09865 (THL1_1988) - 2138587..2140545 (+) 1959 WP_069086464.1 polysaccharide biosynthesis protein -
  THL1_RS09870 (THL1_1989) comE1/comEA 2140605..2140925 (+) 321 WP_069083111.1 ComEA family DNA-binding protein Machinery gene
  THL1_RS09875 (THL1_1990) tnpC 2141039..2141633 (-) 595 Protein_1986 IS66 family transposase -
  THL1_RS09880 (THL1_1991) tnpB 2141710..2142066 (+) 357 WP_003449170.1 IS66 family insertion sequence element accessory protein TnpB -
  THL1_RS09885 (THL1_1992) tnpC 2142086..2143585 (+) 1500 WP_083245827.1 IS66 family transposase -
  THL1_RS09890 (THL1_1993) tnpC 2143630..2144547 (-) 918 Protein_1989 IS66 family transposase -
  THL1_RS09895 (THL1_1994) tnpB 2144567..2144923 (-) 357 WP_003449170.1 IS66 family insertion sequence element accessory protein TnpB -

Sequence


Protein


Download         Length: 106 a.a.        Molecular weight: 11289.02 Da        Isoelectric Point: 6.3116

>NTDB_id=184974 THL1_RS09870 WP_069083111.1 2140605..2140925(+) (comE1/comEA) [Pseudomonas sp. TCU-HL1 isolate Hualien Taiwan]
MIRVRIASLFIVLCSLFVGYSPLAAAAKVETTAASSAEAQAGKLNINAADVVAIQDRLIGIGEAKAKAIVQYREENGPFS
SVDDLLEVKGIGVKTLDRNRDRLSVE

Nucleotide


Download         Length: 321 bp        

>NTDB_id=184974 THL1_RS09870 WP_069083111.1 2140605..2140925(+) (comE1/comEA) [Pseudomonas sp. TCU-HL1 isolate Hualien Taiwan]
ATGATTCGCGTTCGAATCGCCTCGCTGTTTATCGTCCTCTGTTCTCTCTTCGTTGGTTACAGCCCCCTCGCAGCTGCTGC
GAAGGTAGAGACAACTGCCGCTTCAAGTGCTGAAGCACAGGCTGGCAAGCTAAACATCAACGCAGCCGATGTGGTTGCTA
TCCAGGATCGGTTGATTGGTATTGGCGAAGCGAAAGCCAAGGCGATCGTCCAGTACCGCGAGGAGAACGGACCGTTCTCT
TCGGTCGATGACCTGCTTGAGGTCAAGGGGATTGGTGTAAAGACGCTTGATAGGAATCGCGACAGGCTTAGCGTGGAGTA
A

Domains


Predicted by InterproScan.

(41-103)


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A1B3E4S9

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE1/comEA Haemophilus influenzae Rd KW20

46.364

100

0.481

  comEA Vibrio cholerae C6706

43.137

96.226

0.415

  comEA Vibrio cholerae strain A1552

43.137

96.226

0.415

  comE Neisseria gonorrhoeae MS11

43.299

91.509

0.396

  comE Neisseria gonorrhoeae MS11

43.299

91.509

0.396

  comE Neisseria gonorrhoeae MS11

43.299

91.509

0.396

  comE Neisseria gonorrhoeae MS11

43.299

91.509

0.396

  comEA Vibrio parahaemolyticus RIMD 2210633

43.158

89.623

0.387

  comEA Vibrio campbellii strain DS40M4

42.708

90.566

0.387


Multiple sequence alignment