Detailed information NTG0183372

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: LLUL8_RS09190
• Genome accession: NZ_CP015908
• Coordinates: 1819702..1820349 (-)
• Length: 215 a.a.
• NCBI ID: WP_004255240.1
• Uniprot ID: A0A1V0NHR8
• Organism: Lactococcus lactis subsp. lactis strain UL8
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1814702..1825349

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LLUL8_RS09165 (LLUL8_1796)	atpF	1814773..1815279 (-)	507	WP_058221761.1	F0F1 ATP synthase subunit B	-
LLUL8_RS09170 (LLUL8_1797)	atpB	1815294..1816007 (-)	714	WP_004255255.1	F0F1 ATP synthase subunit A	-
LLUL8_RS09175 (LLUL8_1798)	-	1816052..1816267 (-)	216	WP_004255250.1	F0F1 ATP synthase subunit C	-
LLUL8_RS09180 (LLUL8_1799)	-	1816451..1817227 (-)	777	WP_004255244.1	alpha/beta hydrolase family protein	-
LLUL8_RS09185 (LLUL8_1800)	-	1817512..1819721 (-)	2210	Protein_1775	DNA internalization-related competence protein ComEC/Rec2	-
LLUL8_RS09190 (LLUL8_1801)	comEA	1819702..1820349 (-)	648	WP_004255240.1	ComEA family DNA-binding protein	Machinery gene
LLUL8_RS09195 (LLUL8_1802)	-	1820409..1821770 (-)	1362	WP_058221762.1	ABC transporter permease	-
LLUL8_RS09200 (LLUL8_1803)	-	1821767..1822699 (-)	933	WP_004255238.1	ABC transporter ATP-binding protein	-
LLUL8_RS09205 (LLUL8_1804)	-	1822804..1823202 (-)	399	WP_004255236.1	hypothetical protein	-
LLUL8_RS09210 (LLUL8_1805)	-	1823306..1823869 (-)	564	WP_004255233.1	GNAT family N-acetyltransferase	-
LLUL8_RS09215 (LLUL8_1806)	-	1824042..1825220 (-)	1179	WP_021214596.1	SLC13 family permease	-

Sequence

Protein

• Length: 215 a.a.
• Molecular weight: 23651.83 Da
• Isoelectric Point: 5.0062

>NTDB_id=183372 LLUL8_RS09190 WP_004255240.1 1819702..1820349(-) (comEA) [Lactococcus lactis subsp. lactis strain UL8]
MDKILEKVKEYWKMIVLVVCGLIAGGIFYVLTNGQKPTTNLSVENLSSVSRQSSVSKFSEPNEKSVSKIMVDLKGAVTKP
NVYQISSDERLVDLIKEAGGFTDQADQKSINLSAKLKDEEVIYVPKIGENSSTENTDSSANSVTSQVSKTTEKININQAD
LTELQKLTGVGQKKAQDIIDFRTKNGDFKSLEDLGKVSGFGDKTLEKLKDELCFE

Nucleotide

• Length: 648 bp

>NTDB_id=183372 LLUL8_RS09190 WP_004255240.1 1819702..1820349(-) (comEA) [Lactococcus lactis subsp. lactis strain UL8]
ATGGATAAGATTTTAGAAAAAGTAAAAGAATATTGGAAAATGATTGTTTTAGTTGTTTGTGGGCTCATTGCTGGTGGGAT
TTTTTACGTTTTAACCAACGGTCAAAAGCCAACTACAAATCTGTCAGTAGAAAATTTAAGTTCTGTCAGCAGGCAAAGCT
CTGTCAGTAAATTTAGTGAACCTAATGAAAAATCTGTCAGTAAAATTATGGTTGATTTAAAAGGTGCGGTAACAAAGCCT
AATGTCTATCAAATTTCGTCAGATGAACGTCTAGTTGATTTAATTAAAGAAGCTGGTGGTTTTACTGACCAAGCTGACCA
GAAATCAATTAATCTGTCAGCAAAATTAAAAGATGAAGAAGTAATTTATGTCCCAAAAATTGGTGAAAATTCAAGCACAG
AAAATACTGACAGCTCTGCTAATTCGGTCACTAGTCAAGTCTCAAAAACCACTGAAAAAATAAATATCAATCAAGCAGAT
TTGACTGAACTACAAAAATTAACAGGTGTTGGTCAGAAAAAAGCTCAAGACATCATCGATTTTCGGACGAAAAATGGTGA
TTTCAAATCACTTGAAGACTTGGGAAAAGTTTCTGGTTTTGGAGATAAAACACTAGAAAAATTGAAAGATGAGTTGTGTT
TTGAATAA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (70-125)

• Helix-hairpin-helix motif (HHH_3) (151-212)

3D structure

• AlphaFold DB: A0A1V0NHR8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Lactococcus lactis subsp. cremoris KW2	75.688	100	0.767
comEA/celA/cilE	Streptococcus mitis SK321	44.907	100	0.451
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	43.578	100	0.442
comEA/celA/cilE	Streptococcus pneumoniae Rx1	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae D39	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae R6	41.553	100	0.423
comEA/celA/cilE	Streptococcus mitis NCTC 12261	41.743	100	0.423
comEA	Latilactobacillus sakei subsp. sakei 23K	36.564	100	0.386
comEA	Staphylococcus aureus MW2	37.089	99.07	0.367