Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssb   Type   Machinery gene
Locus tag   LLUC77_RS08185 Genome accession   NZ_CP015906
Coordinates   1584979..1585431 (-) Length   150 a.a.
NCBI ID   WP_010905690.1    Uniprot ID   A0AAC9R1D2
Organism   Lactococcus lactis subsp. lactis strain UC77     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1544091..1596631 1584979..1585431 within 0


Gene organization within MGE regions


Location: 1544091..1596631
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LLUC77_RS07880 (LLUC77_1201) nth 1544091..1544747 (-) 657 WP_003130627.1 endonuclease III -
  LLUC77_RS07885 (LLUC77_1200) - 1544769..1545437 (-) 669 WP_010905741.1 DnaD domain-containing protein -
  LLUC77_RS07890 (LLUC77_1198) - 1545844..1547115 (-) 1272 WP_031296484.1 dihydroorotase -
  LLUC77_RS07895 (LLUC77_1197) pyrE 1547270..1547899 (-) 630 WP_003132758.1 orotate phosphoribosyltransferase -
  LLUC77_RS07900 (LLUC77_03170) - 1547880..1548008 (+) 129 WP_003132757.1 hypothetical protein -
  LLUC77_RS07905 (LLUC77_1195) - 1548481..1548642 (-) 162 WP_021214547.1 hypothetical protein -
  LLUC77_RS07910 (LLUC77_1194) - 1548707..1548895 (-) 189 WP_017864422.1 hypothetical protein -
  LLUC77_RS07915 (LLUC77_1193) - 1548929..1549111 (-) 183 WP_003132753.1 hypothetical protein -
  LLUC77_RS07920 (LLUC77_1192) - 1549121..1549852 (-) 732 WP_003132752.1 hypothetical protein -
  LLUC77_RS07925 (LLUC77_1191) - 1550099..1550956 (-) 858 WP_010905739.1 XRE/MutR family transcriptional regulator -
  LLUC77_RS07930 (LLUC77_1190) - 1551131..1551316 (-) 186 WP_003132749.1 hypothetical protein -
  LLUC77_RS07935 (LLUC77_1189) - 1551832..1552197 (-) 366 WP_003132744.1 hypothetical protein -
  LLUC77_RS07940 (LLUC77_1188) - 1552199..1552999 (-) 801 WP_010905736.1 hypothetical protein -
  LLUC77_RS07945 (LLUC77_1187) - 1553188..1555095 (-) 1908 WP_021214548.1 ABC-F family ATP-binding cassette domain-containing protein -
  LLUC77_RS07950 (LLUC77_1186) - 1555273..1555665 (-) 393 WP_003132739.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  LLUC77_RS07955 (LLUC77_1185) - 1555890..1556540 (+) 651 WP_023349143.1 redox-sensing transcriptional repressor Rex -
  LLUC77_RS07960 (LLUC77_1184) - 1557042..1557821 (-) 780 WP_010905733.1 peptidoglycan amidohydrolase family protein -
  LLUC77_RS07965 (LLUC77_1183) - 1557821..1558108 (-) 288 WP_010905732.1 phage holin -
  LLUC77_RS07970 (LLUC77_1182) - 1558121..1558345 (-) 225 WP_010905731.1 hemolysin XhlA family protein -
  LLUC77_RS07975 (LLUC77_1181) - 1558371..1558598 (-) 228 WP_010905730.1 hypothetical protein -
  LLUC77_RS07980 (LLUC77_1180) - 1558600..1558938 (-) 339 WP_010905729.1 hypothetical protein -
  LLUC77_RS07985 (LLUC77_1178) - 1559391..1560221 (-) 831 WP_010905727.1 hypothetical protein -
  LLUC77_RS07990 (LLUC77_1177) - 1560214..1561815 (-) 1602 WP_010905726.1 DUF2479 domain-containing protein -
  LLUC77_RS07995 (LLUC77_1176) - 1561828..1564512 (-) 2685 WP_023349144.1 phage tail spike protein -
  LLUC77_RS08000 (LLUC77_1175) - 1564509..1565258 (-) 750 WP_010905724.1 hypothetical protein -
  LLUC77_RS08005 (LLUC77_1174) - 1565259..1567520 (-) 2262 WP_010905723.1 phage tail tape measure protein -
  LLUC77_RS08010 (LLUC77_03175) - 1567534..1567710 (-) 177 WP_010905722.1 hypothetical protein -
  LLUC77_RS08015 (LLUC77_1173) - 1567737..1568096 (-) 360 WP_010905721.1 hypothetical protein -
  LLUC77_RS08020 (LLUC77_1172) - 1568106..1568714 (-) 609 WP_010905720.1 major tail protein -
  LLUC77_RS08025 (LLUC77_1171) - 1568732..1569049 (-) 318 WP_010905719.1 hypothetical protein -
  LLUC77_RS08030 (LLUC77_1170) - 1569046..1569417 (-) 372 WP_010905718.1 HK97 gp10 family phage protein -
  LLUC77_RS08035 (LLUC77_1169) - 1569407..1569724 (-) 318 WP_010905717.1 phage head closure protein -
  LLUC77_RS08040 (LLUC77_1168) - 1569711..1569992 (-) 282 WP_010905716.1 hypothetical protein -
  LLUC77_RS08045 (LLUC77_1167) - 1569985..1570842 (-) 858 WP_010905715.1 hypothetical protein -
  LLUC77_RS08050 (LLUC77_1166) - 1570897..1572090 (-) 1194 WP_010905714.1 phage major capsid protein -
  LLUC77_RS08055 (LLUC77_1165) - 1572062..1572658 (-) 597 WP_010905713.1 HK97 family phage prohead protease -
  LLUC77_RS08060 (LLUC77_1164) - 1572673..1573878 (-) 1206 WP_010905712.1 phage portal protein -
  LLUC77_RS08065 (LLUC77_1163) - 1573892..1575661 (-) 1770 WP_032941531.1 terminase large subunit -
  LLUC77_RS08070 (LLUC77_1162) - 1575654..1576022 (-) 369 WP_010905710.1 P27 family phage terminase small subunit -
  LLUC77_RS08075 (LLUC77_1161) - 1576114..1576413 (-) 300 WP_023349146.1 HNH endonuclease -
  LLUC77_RS08080 (LLUC77_1160) - 1576677..1577078 (-) 402 WP_011835815.1 DUF722 domain-containing protein -
  LLUC77_RS08085 (LLUC77_1159) - 1577156..1577449 (-) 294 WP_010905707.1 DUF1359 domain-containing protein -
  LLUC77_RS08090 (LLUC77_1157) - 1577813..1577995 (-) 183 WP_010905705.1 hypothetical protein -
  LLUC77_RS08095 (LLUC77_1156) - 1577992..1578291 (-) 300 WP_010905704.1 hypothetical protein -
  LLUC77_RS08100 (LLUC77_1155) - 1578292..1578465 (-) 174 WP_010905703.1 DUF1660 domain-containing protein -
  LLUC77_RS08105 (LLUC77_1154) - 1578507..1578692 (-) 186 WP_010905702.1 hypothetical protein -
  LLUC77_RS08110 (LLUC77_1153) - 1578739..1578903 (-) 165 WP_010905701.1 hypothetical protein -
  LLUC77_RS08115 (LLUC77_1152) - 1578925..1579269 (-) 345 WP_223203818.1 hypothetical protein -
  LLUC77_RS08120 (LLUC77_1151) - 1579262..1579759 (-) 498 WP_010905699.1 hypothetical protein -
  LLUC77_RS08125 (LLUC77_1150) - 1579762..1580181 (-) 420 WP_010905698.1 dUTP diphosphatase -
  LLUC77_RS08130 (LLUC77_1149) - 1580178..1580732 (-) 555 WP_010905358.1 DUF1642 domain-containing protein -
  LLUC77_RS08135 (LLUC77_1148) - 1580729..1581001 (-) 273 WP_010905357.1 hypothetical protein -
  LLUC77_RS08140 (LLUC77_1147) - 1580994..1581200 (-) 207 WP_010905356.1 DUF1125 domain-containing protein -
  LLUC77_RS08145 (LLUC77_1146) - 1581394..1581963 (-) 570 WP_010905697.1 DUF658 family protein -
  LLUC77_RS08150 (LLUC77_1145) - 1581953..1582132 (-) 180 WP_010905696.1 DUF1497 domain-containing protein -
  LLUC77_RS08155 (LLUC77_1144) - 1582154..1582414 (-) 261 WP_223203848.1 hypothetical protein -
  LLUC77_RS08160 (LLUC77_03180) - 1582532..1582771 (-) 240 WP_031297271.1 DUF1031 family protein -
  LLUC77_RS08165 (LLUC77_1143) - 1582764..1582958 (-) 195 WP_010905693.1 hypothetical protein -
  LLUC77_RS08170 (LLUC77_1142) - 1582961..1583350 (-) 390 WP_002819817.1 RusA family crossover junction endodeoxyribonuclease -
  LLUC77_RS08175 (LLUC77_1141) - 1583347..1584072 (-) 726 WP_015966759.1 hypothetical protein -
  LLUC77_RS08180 (LLUC77_1140) - 1584072..1584854 (-) 783 WP_015966758.1 phage replisome organiser protein -
  LLUC77_RS08185 (LLUC77_1139) ssb 1584979..1585431 (-) 453 WP_010905690.1 single-stranded DNA-binding protein Machinery gene
  LLUC77_RS08190 (LLUC77_1138) - 1585431..1586054 (-) 624 WP_010905689.1 DUF1071 domain-containing protein -
  LLUC77_RS08195 (LLUC77_1137) - 1586063..1586581 (-) 519 WP_010905688.1 host-nuclease inhibitor Gam family protein -
  LLUC77_RS08200 (LLUC77_1136) - 1586697..1586912 (-) 216 WP_015966756.1 DUF1408 domain-containing protein -
  LLUC77_RS08205 (LLUC77_1135) - 1587015..1587290 (+) 276 WP_023349243.1 hypothetical protein -
  LLUC77_RS08210 (LLUC77_03185) - 1587287..1587481 (-) 195 WP_010905685.1 hypothetical protein -
  LLUC77_RS08215 (LLUC77_1133) - 1587619..1587954 (-) 336 WP_003131910.1 DUF771 domain-containing protein -
  LLUC77_RS08220 (LLUC77_1132) - 1587967..1588710 (-) 744 WP_010905684.1 ORF6C domain-containing protein -
  LLUC77_RS08225 (LLUC77_1131) - 1588747..1588965 (-) 219 WP_010905683.1 DUF739 family protein -
  LLUC77_RS08230 (LLUC77_1130) - 1589134..1589676 (+) 543 WP_010905682.1 helix-turn-helix domain-containing protein -
  LLUC77_RS08235 (LLUC77_1129) - 1589673..1590110 (+) 438 WP_010905681.1 ImmA/IrrE family metallo-endopeptidase -
  LLUC77_RS08240 (LLUC77_1128) - 1590167..1590712 (+) 546 WP_023349242.1 PH domain-containing protein -
  LLUC77_RS08245 (LLUC77_1127) - 1590835..1591959 (+) 1125 WP_010905679.1 site-specific integrase -
  LLUC77_RS08250 (LLUC77_1126) radC 1592158..1592838 (-) 681 WP_003131898.1 DNA repair protein RadC -
  LLUC77_RS08255 (LLUC77_1125) glmS 1592969..1594786 (-) 1818 WP_081172149.1 glutamine--fructose-6-phosphate transaminase (isomerizing) -
  LLUC77_RS08260 (LLUC77_1124) - 1595093..1595983 (+) 891 WP_043736692.1 IS982-like element ISLll1 family transposase -
  LLUC77_RS08265 (LLUC77_1123) - 1595984..1596631 (-) 648 WP_081172147.1 phosphatase PAP2 family protein -

Sequence


Protein


Download         Length: 150 a.a.        Molecular weight: 16643.67 Da        Isoelectric Point: 8.9728

>NTDB_id=183270 LLUC77_RS08185 WP_010905690.1 1584979..1585431(-) (ssb) [Lactococcus lactis subsp. lactis strain UC77]
MINNVVLVGRLTRDPELRHTPQNQAVGTFGLAVNRQFKNANGEREADFINCVIWRQQAENLAKFAKKGALIGITGRIQTR
NYENQQGQKVYVTEVVADTFQMLESNKTQGQQTSKPQAQNKKPQAPDPFKAPAADPFAGGTEISDDDLPF

Nucleotide


Download         Length: 453 bp        

>NTDB_id=183270 LLUC77_RS08185 WP_010905690.1 1584979..1585431(-) (ssb) [Lactococcus lactis subsp. lactis strain UC77]
ATGATTAATAATGTTGTATTAGTAGGTCGCTTAACTCGTGACCCTGAACTTAGACATACACCACAGAACCAAGCAGTAGG
TACATTTGGATTAGCTGTAAATCGCCAGTTTAAAAATGCGAATGGCGAACGTGAGGCTGATTTCATTAACTGCGTTATTT
GGCGACAACAAGCCGAAAATTTAGCTAAGTTTGCTAAAAAAGGAGCTTTGATTGGAATAACTGGACGGATTCAAACAAGG
AACTATGAGAACCAACAAGGACAAAAAGTTTATGTTACCGAAGTTGTTGCAGATACTTTCCAAATGCTAGAAAGCAATAA
AACACAAGGTCAGCAAACAAGTAAACCGCAAGCTCAAAATAAAAAGCCACAAGCACCAGACCCTTTTAAAGCTCCTGCTG
CTGATCCATTTGCTGGTGGTACTGAAATTTCAGATGATGACCTACCATTTTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Latilactobacillus sakei subsp. sakei 23K

58.14

100

0.667

  ssbA Bacillus subtilis subsp. subtilis str. 168

52.907

100

0.607

  ssbB Bacillus subtilis subsp. subtilis str. 168

58.654

69.333

0.407

  ssb Neisseria meningitidis MC58

34.302

100

0.393

  ssb Neisseria gonorrhoeae MS11

33.721

100

0.387

  ssbB Streptococcus sobrinus strain NIDR 6715-7

52.381

70

0.367

  ssbB/cilA Streptococcus pneumoniae TIGR4

51.429

70

0.36


Multiple sequence alignment