Detailed information NTG0183084

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: LLUC06_RS09985
• Genome accession: NZ_CP015902
• Coordinates: 1999244..1999891 (-)
• Length: 215 a.a.
• NCBI ID: WP_081213665.1
• Uniprot ID: A0A1V0P448
• Organism: Lactococcus lactis subsp. lactis strain UC06
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1994244..2004891

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LLUC06_RS09960 (LLUC06_1940)	atpF	1994313..1994819 (-)	507	WP_081213662.1	F0F1 ATP synthase subunit B	-
LLUC06_RS09965 (LLUC06_1941)	atpB	1994834..1995547 (-)	714	WP_081213663.1	F0F1 ATP synthase subunit A	-
LLUC06_RS09970 (LLUC06_1942)	-	1995592..1995807 (-)	216	WP_004255250.1	F0F1 ATP synthase subunit C	-
LLUC06_RS09975 (LLUC06_1943)	-	1995992..1996768 (-)	777	WP_058220836.1	alpha/beta hydrolase family protein	-
LLUC06_RS09980 (LLUC06_1944)	comEC	1997053..1999263 (-)	2211	WP_081213664.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
LLUC06_RS09985 (LLUC06_1945)	comEA	1999244..1999891 (-)	648	WP_081213665.1	ComEA family DNA-binding protein	Machinery gene
LLUC06_RS09990 (LLUC06_1946)	-	1999950..2001311 (-)	1362	WP_058220838.1	ABC transporter permease	-
LLUC06_RS09995 (LLUC06_1947)	-	2001308..2002240 (-)	933	WP_004255238.1	ABC transporter ATP-binding protein	-
LLUC06_RS10000 (LLUC06_1948)	-	2002345..2002743 (-)	399	WP_081213666.1	hypothetical protein	-
LLUC06_RS10005 (LLUC06_1949)	-	2002847..2003410 (-)	564	WP_004255233.1	GNAT family N-acetyltransferase	-
LLUC06_RS10010 (LLUC06_1950)	-	2003583..2004761 (-)	1179	WP_012898345.1	SLC13 family permease	-

Sequence

Protein

• Length: 215 a.a.
• Molecular weight: 23633.86 Da
• Isoelectric Point: 5.1791

>NTDB_id=183084 LLUC06_RS09985 WP_081213665.1 1999244..1999891(-) (comEA) [Lactococcus lactis subsp. lactis strain UC06]
MDKILEKVKEYWKMIVLVVCGLIAGGIFYVLTNGQKPTTNLSVENLSSVSRQSSVSKFSEPNKKSVSKIMVDLKGAVTKP
NVYQISPDERLVDLIKEAGGFTDQADQKSINLSAKLKDEEVIYVPKIGENSSTENTDSSANSVTSQVSTTTEKININQAD
LTELQKLTGVGQKKAQDIIDFRTKNGDFKSLEDLGKVSGFGDKTLEKLKDELCFE

Nucleotide

• Length: 648 bp

>NTDB_id=183084 LLUC06_RS09985 WP_081213665.1 1999244..1999891(-) (comEA) [Lactococcus lactis subsp. lactis strain UC06]
ATGGATAAGATTTTAGAAAAAGTAAAAGAATATTGGAAAATGATTGTTTTAGTTGTTTGTGGGCTCATTGCTGGTGGGAT
TTTTTACGTTTTAACCAACGGTCAAAAGCCAACTACAAATCTGTCAGTAGAAAATTTAAGTTCTGTCAGCAGGCAAAGCT
CTGTCAGTAAATTTAGTGAACCTAATAAAAAATCTGTCAGTAAAATTATGGTTGATTTAAAAGGTGCGGTAACAAAGCCT
AATGTCTATCAAATTTCGCCAGATGAACGTCTAGTTGATCTAATTAAGGAAGCTGGTGGTTTTACTGACCAAGCTGACCA
GAAATCAATTAATCTGTCAGCAAAATTAAAAGATGAAGAAGTAATTTATGTCCCAAAAATTGGTGAAAATTCAAGCACAG
AAAATACTGATAGCTCTGCTAATTCGGTCACTAGTCAAGTCTCAACAACCACTGAAAAAATAAATATCAATCAAGCAGAT
TTGACTGAACTACAAAAATTAACAGGTGTTGGTCAGAAAAAAGCTCAAGACATCATTGATTTTCGGACGAAAAATGGTGA
TTTCAAATCACTTGAAGACTTGGGAAAAGTTTCTGGTTTTGGAGATAAAACACTAGAAAAATTGAAAGATGAGTTGTGTT
TTGAATAA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (70-125)

• Helix-hairpin-helix motif (HHH_3) (151-212)

3D structure

• AlphaFold DB: A0A1V0P448

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Lactococcus lactis subsp. cremoris KW2	75.229	100	0.763
comEA/celA/cilE	Streptococcus mitis SK321	44.444	100	0.447
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	43.119	100	0.437
comEA/celA/cilE	Streptococcus pneumoniae Rx1	41.096	100	0.419
comEA/celA/cilE	Streptococcus pneumoniae D39	41.096	100	0.419
comEA/celA/cilE	Streptococcus pneumoniae R6	41.096	100	0.419
comEA/celA/cilE	Streptococcus mitis NCTC 12261	41.284	100	0.419
comEA	Latilactobacillus sakei subsp. sakei 23K	36.564	100	0.386