Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   LLJM1_RS06845 Genome accession   NZ_CP015899
Coordinates   1264146..1264994 (+) Length   282 a.a.
NCBI ID   WP_032950088.1    Uniprot ID   Q25AX2
Organism   Lactococcus cremoris strain JM1     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1259146..1269994
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LLJM1_RS06820 (LLJM1_1305) ilvN 1259879..1260355 (+) 477 WP_032950082.1 acetolactate synthase small subunit -
  LLJM1_RS06825 (LLJM1_1306) ilvC 1260401..1261423 (+) 1023 WP_021211415.1 ketol-acid reductoisomerase -
  LLJM1_RS06830 (LLJM1_1307) ilvA 1261482..1262732 (+) 1251 WP_032950084.1 threonine ammonia-lyase IlvA -
  LLJM1_RS06835 (LLJM1_1308) budA 1262867..1263577 (+) 711 WP_011676302.1 acetolactate decarboxylase -
  LLJM1_RS06840 (LLJM1_1309) - 1263617..1263997 (+) 381 WP_014572603.1 RidA family protein -
  LLJM1_RS06845 (LLJM1_1310) dprA 1264146..1264994 (+) 849 WP_032950088.1 DNA-processing protein DprA Machinery gene
  LLJM1_RS06850 (LLJM1_1311) topA 1265260..1267386 (+) 2127 WP_015082465.1 type I DNA topoisomerase -
  LLJM1_RS06855 (LLJM1_1312) trmFO 1267458..1268804 (+) 1347 WP_032950090.1 methylenetetrahydrofolate--tRNA-(uracil(54)- C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO -
  LLJM1_RS06860 (LLJM1_1313) xerS 1268854..1269924 (-) 1071 WP_014572599.1 tyrosine recombinase XerS -

Sequence

Protein


Download         Length: 282 a.a.        Molecular weight: 31955.09 Da        Isoelectric Point: 9.8385

>NTDB_id=182936 LLJM1_RS06845 WP_032950088.1 1264146..1264994(+) (dprA) [Lactococcus cremoris strain JM1]
MITNFDLFRWKKAGMTNLGVNKLLKFFRKYDIKISLRQMGQVAQVKSIPNFIEQYKNQDVKKLRIDYKKFSSFSILDDLY
PERLREIYNPPVLIFYQGNIDLLKNPKLAFVGSRLAGQSGIKAVQKIITELNQSFTIVSGLAKGIDTASHLSAIKTKTPT
IAVIGTGLDIFYPLENRKIQEYLAKNQLVLSEYSLGEKPLKYHFPERNRIIAGLSRGVVVVEAKLRSGSLITCERALEEG
RDIFAIPGNIADGTSDDCNHLIQQGAKLVYQAQDILEEYLYN

Nucleotide


Download         Length: 849 bp        

>NTDB_id=182936 LLJM1_RS06845 WP_032950088.1 1264146..1264994(+) (dprA) [Lactococcus cremoris strain JM1]
ATGATTACAAATTTTGATTTATTTCGTTGGAAAAAAGCAGGAATGACCAATCTTGGTGTAAATAAGCTCTTAAAATTTTT
CCGCAAATATGATATAAAAATAAGCTTACGACAAATGGGACAAGTGGCTCAAGTCAAATCAATCCCTAACTTTATCGAAC
AATATAAAAATCAGGACGTAAAGAAACTCAGGATAGACTATAAAAAATTTTCCTCCTTTTCCATTCTTGATGACCTTTAT
CCCGAGCGTCTAAGAGAAATCTACAACCCACCCGTTTTAATTTTTTATCAGGGAAATATTGATTTATTAAAAAATCCTAA
ACTTGCCTTTGTTGGAAGCCGCCTAGCAGGTCAATCAGGGATAAAAGCTGTCCAAAAAATTATCACAGAATTGAATCAAT
CCTTTACAATAGTAAGCGGGCTTGCTAAAGGGATTGACACAGCCAGCCATCTGTCAGCAATTAAAACTAAAACACCAACA
ATTGCTGTTATTGGTACAGGATTGGATATTTTCTATCCACTAGAAAATCGTAAAATACAGGAATATCTAGCAAAAAATCA
ATTAGTTTTAAGTGAATATTCATTAGGCGAAAAACCACTTAAATACCACTTTCCAGAGCGCAATCGAATTATTGCTGGTC
TTTCACGGGGAGTTGTTGTCGTGGAAGCAAAATTAAGAAGTGGCAGTTTAATCACCTGTGAAAGGGCACTGGAAGAAGGA
AGAGATATCTTTGCCATCCCTGGAAATATTGCAGATGGAACATCTGATGACTGTAATCATCTTATTCAACAAGGAGCAAA
ACTTGTCTATCAAGCTCAAGATATTTTAGAAGAATATTTGTACAATTAA

Domains

Predicted by InterproScan.

DNA recombination-mediator protein A (DNA_processg_A)

(69-270)

DNA processing protein A sterile alpha motif domain (SAM_DprA)

(2-63)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q25AX2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Lactococcus lactis subsp. cremoris KW2

97.872

100

0.979
  dprA Streptococcus mutans UA159

58.993

98.582

0.582
  dprA/cilB/dalA Streptococcus mitis SK321

55.714

99.291

0.553
  dprA/cilB/dalA Streptococcus pneumoniae R6

55.357

99.291

0.55
  dprA/cilB/dalA Streptococcus pneumoniae TIGR4

55.357

99.291

0.55
  dprA/cilB/dalA Streptococcus mitis NCTC 12261

55.357

99.291

0.55
  dprA/cilB/dalA Streptococcus pneumoniae Rx1

55.357

99.291

0.55
  dprA/cilB/dalA Streptococcus pneumoniae D39

55.357

99.291

0.55
  dprA Vibrio campbellii strain DS40M4

35.314

100

0.379
  dprA Bacillus subtilis subsp. subtilis str. 168

38.909

97.518

0.379
  dprA Legionella pneumophila strain ERS1305867

38.768

97.872

0.379
  dprA Haemophilus influenzae Rd KW20

39.259

95.745

0.376

Multiple sequence alignment