Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   LLJM1_RS00760 Genome accession   NZ_CP015899
Coordinates   149533..150558 (+) Length   341 a.a.
NCBI ID   WP_050595614.1    Uniprot ID   A0A1V0PEJ1
Organism   Lactococcus cremoris strain JM1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 144533..155558
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LLJM1_RS00750 (LLJM1_0133) - 144708..148538 (+) 3831 Protein_138 PolC-type DNA polymerase III -
  LLJM1_RS00755 (LLJM1_0134) comGA 148653..149633 (+) 981 WP_032951299.1 competence type IV pilus ATPase ComGA Machinery gene
  LLJM1_RS00760 (LLJM1_0135) comGB 149533..150558 (+) 1026 WP_050595614.1 competence type IV pilus assembly protein ComGB Machinery gene
  LLJM1_RS00765 (LLJM1_0136) comGC 150603..150953 (+) 351 WP_050574187.1 competence type IV pilus major pilin ComGC Machinery gene
  LLJM1_RS00770 (LLJM1_0137) comGD 151155..151343 (+) 189 WP_014573336.1 hypothetical protein Machinery gene
  LLJM1_RS00775 (LLJM1_0138) comGE 151375..151611 (+) 237 WP_014573335.1 competence type IV pilus minor pilin ComGE Machinery gene
  LLJM1_RS00780 (LLJM1_0139) comGF 151574..152020 (+) 447 WP_011836043.1 competence type IV pilus minor pilin ComGF Machinery gene
  LLJM1_RS00785 (LLJM1_02515) comGG 152116..152343 (+) 228 WP_228764408.1 competence protein ComGG Machinery gene
  LLJM1_RS00790 (LLJM1_0141) - 152423..152860 (+) 438 WP_011677180.1 zinc-dependent MarR family transcriptional regulator -
  LLJM1_RS00795 (LLJM1_0142) - 152857..153699 (+) 843 WP_011677179.1 metal ABC transporter solute-binding protein, Zn/Mn family -
  LLJM1_RS00800 (LLJM1_0143) - 153878..154615 (+) 738 WP_011677178.1 metal ABC transporter ATP-binding protein -
  LLJM1_RS00805 (LLJM1_0144) - 154608..155417 (+) 810 WP_011677177.1 metal ABC transporter permease -

Sequence

Protein


Download         Length: 341 a.a.        Molecular weight: 38420.20 Da        Isoelectric Point: 9.9840

>NTDB_id=182911 LLJM1_RS00760 WP_050595614.1 149533..150558(+) (comGB) [Lactococcus cremoris strain JM1]
MQTDILRLLRPKGKKLALIKQAKLIQLMGNLLNNGFHLGEVINFLALSKLVEKEFVFKMHQGLSSGNSLSEILDSLSCSK
NVVTQLALVEVHGNLVGTMQLVELHLKKQLKVKKKLIEVATYPVVLLVFLIGIMWGLKNYLLPQIDKGNNFATLLINHLP
LVFFSVGALIVLLTSLSIIIFKHLSALTNFTFLVKVPLVSSFIRLYLTAYFSREWGNLIAQGVELRQIIELMKKQKIRIF
AEVGIKLDLGLKAGQNFEQAVNHFSIFLPELSLMIEYGAIKDKLGLELSVYADECWEQFFSKIDRLMQLIQPLIFIFVAL
MIVLLYAAMSLPIYSNMGSGI

Nucleotide


Download         Length: 1026 bp        

>NTDB_id=182911 LLJM1_RS00760 WP_050595614.1 149533..150558(+) (comGB) [Lactococcus cremoris strain JM1]
ATGCAAACGGACATCTTACGGCTATTGAGGCCGAAAGGGAAAAAATTAGCATTAATCAAGCAAGCAAAATTGATACAACT
GATGGGGAATCTTTTGAATAATGGTTTCCATTTGGGAGAAGTTATCAATTTTTTAGCTTTATCAAAATTAGTAGAAAAAG
AGTTTGTATTCAAAATGCACCAAGGTCTCAGTTCTGGAAATTCATTATCTGAAATTTTAGATAGTCTCTCTTGTTCTAAA
AATGTTGTGACACAACTTGCTTTGGTAGAAGTGCATGGGAATTTAGTAGGAACCATGCAATTGGTAGAACTGCATTTAAA
AAAGCAGCTTAAGGTCAAAAAGAAATTAATTGAAGTGGCAACTTATCCAGTTGTATTGTTGGTATTTCTCATTGGAATTA
TGTGGGGCTTGAAAAATTATTTATTACCTCAAATTGATAAGGGAAATAATTTTGCTACTTTATTAATTAATCATTTACCG
CTAGTATTTTTTTCTGTTGGTGCTTTAATCGTTTTACTGACAAGTTTATCAATAATAATATTCAAACATCTTTCAGCACT
CACTAATTTTACTTTTTTGGTTAAAGTTCCATTAGTAAGTTCTTTTATCCGCTTGTATTTGACCGCTTATTTCTCCAGAG
AATGGGGAAATTTAATTGCTCAAGGAGTTGAATTGCGACAAATAATAGAATTGATGAAAAAGCAGAAAATTCGCATTTTT
GCGGAAGTTGGAATTAAACTTGATTTGGGTTTGAAGGCTGGTCAGAATTTTGAGCAAGCTGTCAATCATTTTTCGATTTT
TTTACCAGAACTTTCTTTGATGATTGAGTACGGCGCGATTAAAGATAAATTAGGGTTGGAGCTTTCTGTTTATGCTGATG
AATGTTGGGAACAATTCTTTTCTAAGATAGACCGTCTAATGCAATTAATACAACCCCTTATTTTTATTTTTGTTGCTTTG
ATGATTGTCTTATTGTATGCAGCGATGTCGTTACCCATTTATTCTAATATGGGTTCTGGAATTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(211-332)

Type II secretion system (T2SS), protein F (T2SSF)

(25-143)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1V0PEJ1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Lactococcus lactis subsp. cremoris KW2

97.361

100

0.974
  comYB Streptococcus gordonii str. Challis substr. CH1

51.039

98.827

0.504
  comGB/cglB Streptococcus pneumoniae D39

50.299

97.947

0.493
  comGB/cglB Streptococcus pneumoniae R6

50.299

97.947

0.493
  comGB/cglB Streptococcus pneumoniae Rx1

50.299

97.947

0.493
  comGB/cglB Streptococcus pneumoniae TIGR4

50.299

97.947

0.493
  comGB/cglB Streptococcus mitis SK321

50

97.947

0.49
  comGB/cglB Streptococcus mitis NCTC 12261

49.102

97.947

0.481
  comYB Streptococcus mutans UA140

50.955

92.082

0.469
  comYB Streptococcus mutans UA159

50.318

92.082

0.463

Multiple sequence alignment