Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   LLJM1_RS00755 Genome accession   NZ_CP015899
Coordinates   148653..149633 (+) Length   326 a.a.
NCBI ID   WP_032951299.1    Uniprot ID   A0A1V0PEA7
Organism   Lactococcus cremoris strain JM1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 143653..154633
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LLJM1_RS00750 (LLJM1_0133) - 144708..148538 (+) 3831 Protein_138 PolC-type DNA polymerase III -
  LLJM1_RS00755 (LLJM1_0134) comGA 148653..149633 (+) 981 WP_032951299.1 competence type IV pilus ATPase ComGA Machinery gene
  LLJM1_RS00760 (LLJM1_0135) comGB 149533..150558 (+) 1026 WP_050595614.1 competence type IV pilus assembly protein ComGB Machinery gene
  LLJM1_RS00765 (LLJM1_0136) comGC 150603..150953 (+) 351 WP_050574187.1 competence type IV pilus major pilin ComGC Machinery gene
  LLJM1_RS00770 (LLJM1_0137) comGD 151155..151343 (+) 189 WP_014573336.1 hypothetical protein Machinery gene
  LLJM1_RS00775 (LLJM1_0138) comGE 151375..151611 (+) 237 WP_014573335.1 competence type IV pilus minor pilin ComGE Machinery gene
  LLJM1_RS00780 (LLJM1_0139) comGF 151574..152020 (+) 447 WP_011836043.1 competence type IV pilus minor pilin ComGF Machinery gene
  LLJM1_RS00785 (LLJM1_02515) comGG 152116..152343 (+) 228 WP_228764408.1 competence protein ComGG Machinery gene
  LLJM1_RS00790 (LLJM1_0141) - 152423..152860 (+) 438 WP_011677180.1 zinc-dependent MarR family transcriptional regulator -
  LLJM1_RS00795 (LLJM1_0142) - 152857..153699 (+) 843 WP_011677179.1 metal ABC transporter solute-binding protein, Zn/Mn family -
  LLJM1_RS00800 (LLJM1_0143) - 153878..154615 (+) 738 WP_011677178.1 metal ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 37063.37 Da        Isoelectric Point: 5.9464

>NTDB_id=182910 LLJM1_RS00755 WP_032951299.1 148653..149633(+) (comGA) [Lactococcus cremoris strain JM1]
MVQKKAQELIQKAIEMEASDIYLIASGNLYKIYIRQQLGRTLIEELNQEIGLALLTHFKFLAGMNTGERRRVQLGACWYE
LEGSSAKRLRLSTVGDFEGNESLVIRLLHDQKQELEFWFDDKLQDFRCKRGLYLFSGPVGSGKTSLMFDLSHRHFSNAQV
ITIEEPVELIESDFIQLQVNDVIGNSYDELIKLSLRHRPDLLIVGEIRDQQTARAVLRASLTGYTVFSTIHAASVKGVVQ
RLLELGLSDWELKNGLEAVVYQRLIVGKGVLDIAKSKFDTWSPKKWNEKIENLYANGHLTAIEAEREKISINQASKIDTT
DGESFE

Nucleotide


Download         Length: 981 bp        

>NTDB_id=182910 LLJM1_RS00755 WP_032951299.1 148653..149633(+) (comGA) [Lactococcus cremoris strain JM1]
ATGGTACAGAAAAAAGCACAAGAACTCATTCAAAAGGCAATTGAGATGGAGGCTTCTGATATTTATTTAATTGCTTCAGG
AAATCTTTATAAGATATATATTCGTCAACAATTAGGGCGAACTTTAATAGAGGAACTTAACCAAGAGATTGGTTTAGCAT
TACTTACTCATTTTAAATTTCTTGCTGGCATGAATACTGGTGAACGCCGGCGTGTTCAGCTGGGTGCTTGTTGGTATGAA
CTAGAGGGAAGTTCGGCAAAACGTTTGCGCCTTTCAACAGTGGGAGATTTTGAAGGAAATGAATCATTGGTTATCCGTCT
TTTGCATGACCAAAAACAAGAACTTGAGTTTTGGTTTGACGATAAACTTCAGGATTTTCGATGTAAACGAGGACTTTATT
TATTTTCTGGGCCAGTAGGGTCTGGGAAAACTTCACTAATGTTTGACTTATCCCACCGTCATTTTTCAAATGCACAGGTT
ATCACTATTGAGGAGCCTGTAGAATTAATTGAATCTGATTTTATTCAGTTACAAGTTAATGATGTAATTGGGAATAGTTA
TGATGAATTAATTAAACTTTCTTTAAGACACCGACCAGATTTACTAATTGTTGGAGAAATTCGAGATCAGCAGACAGCTC
GTGCTGTATTGCGTGCAAGTTTAACAGGCTATACAGTTTTTTCTACTATCCATGCGGCTTCCGTGAAGGGAGTGGTTCAA
CGTTTGTTGGAATTGGGTTTGAGTGATTGGGAATTGAAAAATGGATTAGAGGCAGTTGTTTATCAACGGTTAATAGTGGG
AAAAGGAGTATTAGATATTGCGAAAAGTAAATTTGACACTTGGTCGCCGAAAAAATGGAATGAAAAGATTGAGAACTTAT
ATGCAAACGGACATCTTACGGCTATTGAGGCCGAAAGGGAAAAAATTAGCATTAATCAAGCAAGCAAAATTGATACAACT
GATGGGGAATCTTTTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1V0PEA7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Lactococcus lactis subsp. cremoris KW2

98.466

100

0.985
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

56.452

95.092

0.537
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

54.808

95.706

0.525
  comGA/cglA/cilD Streptococcus pneumoniae D39

54.808

95.706

0.525
  comGA/cglA/cilD Streptococcus pneumoniae R6

54.808

95.706

0.525
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

54.808

95.706

0.525
  comYA Streptococcus gordonii str. Challis substr. CH1

54.114

96.933

0.525
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

54.167

95.706

0.518
  comYA Streptococcus mutans UA159

52.733

95.399

0.503
  comYA Streptococcus mutans UA140

52.733

95.399

0.503

Multiple sequence alignment