Detailed information NTG0182886

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: LL275_RS09435
• Genome accession: NZ_CP015897
• Coordinates: 1901825..1902472 (-)
• Length: 215 a.a.
• NCBI ID: WP_004255240.1
• Uniprot ID: A0A1V0NHR8
• Organism: Lactococcus lactis subsp. lactis strain 275
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1896825..1907472

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LL275_RS09410 (LL275_1823)	atpF	1896895..1897401 (-)	507	WP_004255258.1	F0F1 ATP synthase subunit B	-
LL275_RS09415 (LL275_1824)	atpB	1897416..1898129 (-)	714	WP_004255255.1	F0F1 ATP synthase subunit A	-
LL275_RS09420 (LL275_1825)	-	1898174..1898389 (-)	216	WP_004255250.1	F0F1 ATP synthase subunit C	-
LL275_RS09425 (LL275_1826)	-	1898573..1899349 (-)	777	WP_004255244.1	alpha/beta hydrolase family protein	-
LL275_RS09430 (LL275_1827)	comEC	1899634..1901844 (-)	2211	WP_081144329.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
LL275_RS09435 (LL275_1828)	comEA	1901825..1902472 (-)	648	WP_004255240.1	ComEA family DNA-binding protein	Machinery gene
LL275_RS09440 (LL275_1829)	-	1902532..1903893 (-)	1362	WP_004255239.1	ABC transporter permease	-
LL275_RS09445 (LL275_1830)	-	1903890..1904822 (-)	933	WP_004255238.1	ABC transporter ATP-binding protein	-
LL275_RS09450 (LL275_1831)	-	1904927..1905325 (-)	399	WP_004255236.1	hypothetical protein	-
LL275_RS09455 (LL275_1832)	-	1905429..1905992 (-)	564	WP_004255233.1	GNAT family N-acetyltransferase	-
LL275_RS09460 (LL275_1833)	-	1906165..1907343 (-)	1179	WP_021214596.1	SLC13 family permease	-

Sequence

Protein

• Length: 215 a.a.
• Molecular weight: 23651.83 Da
• Isoelectric Point: 5.0062

>NTDB_id=182886 LL275_RS09435 WP_004255240.1 1901825..1902472(-) (comEA) [Lactococcus lactis subsp. lactis strain 275]
MDKILEKVKEYWKMIVLVVCGLIAGGIFYVLTNGQKPTTNLSVENLSSVSRQSSVSKFSEPNEKSVSKIMVDLKGAVTKP
NVYQISSDERLVDLIKEAGGFTDQADQKSINLSAKLKDEEVIYVPKIGENSSTENTDSSANSVTSQVSKTTEKININQAD
LTELQKLTGVGQKKAQDIIDFRTKNGDFKSLEDLGKVSGFGDKTLEKLKDELCFE

Nucleotide

• Length: 648 bp

>NTDB_id=182886 LL275_RS09435 WP_004255240.1 1901825..1902472(-) (comEA) [Lactococcus lactis subsp. lactis strain 275]
ATGGATAAGATTTTAGAAAAAGTAAAAGAATATTGGAAAATGATTGTTTTAGTTGTTTGTGGGCTCATTGCTGGTGGGAT
TTTTTACGTTTTAACCAACGGTCAAAAGCCAACTACAAATCTGTCAGTAGAAAATTTAAGTTCTGTCAGCAGGCAAAGCT
CTGTCAGTAAATTTAGTGAACCTAATGAAAAATCTGTCAGTAAAATTATGGTTGATTTAAAAGGTGCGGTAACAAAGCCT
AATGTCTATCAAATTTCGTCAGATGAACGTCTAGTTGATTTAATTAAAGAAGCTGGTGGTTTTACTGACCAAGCTGACCA
GAAATCAATTAATCTGTCAGCAAAATTAAAAGATGAAGAAGTAATTTATGTCCCAAAAATTGGTGAAAATTCAAGCACAG
AAAATACTGACAGCTCTGCTAATTCGGTCACTAGTCAAGTCTCAAAAACCACTGAAAAAATAAATATCAATCAAGCAGAT
TTGACTGAACTACAAAAATTAACAGGTGTTGGTCAGAAAAAAGCTCAAGACATCATCGATTTTCGGACGAAAAATGGTGA
TTTCAAATCACTTGAAGACTTGGGAAAAGTTTCTGGTTTTGGAGATAAAACACTAGAAAAATTGAAAGATGAGTTGTGTT
TTGAATAA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (70-125)

• Helix-hairpin-helix motif (HHH_3) (151-212)

3D structure

• AlphaFold DB: A0A1V0NHR8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Lactococcus lactis subsp. cremoris KW2	75.688	100	0.767
comEA/celA/cilE	Streptococcus mitis SK321	44.907	100	0.451
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	43.578	100	0.442
comEA/celA/cilE	Streptococcus pneumoniae Rx1	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae D39	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae R6	41.553	100	0.423
comEA/celA/cilE	Streptococcus mitis NCTC 12261	41.743	100	0.423
comEA	Latilactobacillus sakei subsp. sakei 23K	36.564	100	0.386
comEA	Staphylococcus aureus MW2	37.089	99.07	0.367