Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   A8L59_RS02045 Genome accession   NZ_CP015852
Coordinates   459036..461111 (+) Length   691 a.a.
NCBI ID   WP_064585600.1    Uniprot ID   A0AAC9BNU6
Organism   Pseudomonas koreensis strain CRS05-R5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 454036..466111
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A8L59_RS02025 (A8L59_02020) pilM 456248..457312 (+) 1065 WP_064585596.1 pilus assembly protein PilM Machinery gene
  A8L59_RS02030 (A8L59_02025) - 457312..457878 (+) 567 WP_064585597.1 PilN domain-containing protein -
  A8L59_RS02035 (A8L59_02030) pilO 457875..458498 (+) 624 WP_064585598.1 type 4a pilus biogenesis protein PilO -
  A8L59_RS02040 (A8L59_02035) - 458495..459022 (+) 528 WP_064585599.1 pilus assembly protein PilP -
  A8L59_RS02045 (A8L59_02040) pilQ 459036..461111 (+) 2076 WP_064585600.1 type IV pilus secretin PilQ Machinery gene
  A8L59_RS02050 (A8L59_02045) aroK 461116..461634 (+) 519 WP_027611316.1 shikimate kinase AroK -
  A8L59_RS02055 (A8L59_02050) aroB 461786..462886 (+) 1101 WP_064585601.1 3-dehydroquinate synthase -
  A8L59_RS02060 (A8L59_02055) - 462897..464486 (+) 1590 WP_064585602.1 AAA family ATPase -

Sequence

Protein


Download         Length: 691 a.a.        Molecular weight: 74973.27 Da        Isoelectric Point: 5.5724

>NTDB_id=182492 A8L59_RS02045 WP_064585600.1 459036..461111(+) (pilQ) [Pseudomonas koreensis strain CRS05-R5]
MNRIFSTLGFSLWIALLSPMVQAASLKALDVAALPGDRVELKLSFDGPPPQPKGYTTESPARIALDLPGVASQLTSKTRD
LGSGNARSATMVEANDRTRLIINLTQLTPYTARVEGNNLFVVVGQGAKPAAPRPATAVAPRPAAAPTKAYAPTVKAIRGV
DFQRGTAGEGNVVIDLSDPTIAPDIQEHDGKIILSFARTQLPDRLRVRLDVKDFATPVQYVNSAATGDRAVITVEPSGTF
DYSTYQTDNKLTVSIRPMTVDDLQKRNADRQAYSGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQNVP
WDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFQSVTSAEAKI
DERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNKGNWNTSGVSNG
TSTTIGTPGSTSTNSPFVDMGTVGNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPYQ
EASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFSNT
QSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2076 bp        

>NTDB_id=182492 A8L59_RS02045 WP_064585600.1 459036..461111(+) (pilQ) [Pseudomonas koreensis strain CRS05-R5]
ATGAACAGGATTTTCTCCACCCTCGGTTTTTCGCTATGGATAGCGCTGCTTTCACCGATGGTTCAGGCGGCCAGCCTCAA
GGCGCTGGATGTGGCGGCGTTACCCGGCGATCGCGTCGAGTTGAAGCTGTCGTTCGACGGCCCGCCCCCACAGCCCAAGG
GCTACACCACTGAGTCACCGGCGCGAATCGCGCTGGATCTGCCGGGTGTCGCCAGCCAGCTGACGAGCAAGACCCGCGAT
CTGGGCAGCGGCAACGCACGCAGCGCCACGATGGTCGAGGCCAATGATCGGACGCGCCTGATCATCAACCTGACGCAACT
GACGCCCTACACCGCGCGGGTCGAAGGCAACAATTTGTTCGTGGTGGTCGGGCAGGGTGCCAAACCGGCTGCACCACGGC
CGGCCACTGCGGTTGCGCCACGCCCGGCCGCGGCGCCGACGAAGGCTTACGCACCCACCGTCAAGGCCATTCGCGGCGTC
GACTTCCAGCGGGGTACGGCAGGAGAGGGCAACGTGGTCATCGACCTGTCGGACCCGACCATCGCCCCTGACATTCAGGA
GCATGACGGCAAGATCATCCTCAGTTTTGCCCGCACCCAGTTGCCCGACCGCTTGCGAGTACGTCTCGACGTCAAGGATT
TCGCCACCCCGGTGCAGTACGTCAACAGCGCCGCAACTGGCGATCGCGCAGTCATCACTGTCGAACCCAGCGGCACGTTC
GACTATTCGACCTACCAGACCGACAACAAGCTGACCGTCAGCATTCGCCCGATGACGGTCGACGACCTGCAAAAGCGTAA
TGCCGACCGCCAGGCCTACAGTGGCGAAAAGCTCTCGCTGAATTTCCAGGACATCGATGTGCGTTCGGTGCTGCAACTGA
TCGCCGACTTCACCAACCTGAATCTGGTGGCCAGCGACACGGTGCAGGGCGGTATCACCTTGCGTCTGCAGAATGTGCCG
TGGGATCAGGCGCTGGATCTGGTGCTGAAAACCAAAGGCCTGGATAAACGCAAGATCGGCAACGTATTGCTCGTGGCGCC
GGCTGACGAAATCGCCGCGCGCGAACGCCAGGAACTGGAGTCGCAGAAGCAGATCGCTGAACTGGCACCACTGCGCCGCG
AGTTGCTGCAGGTGAACTACGCCAAGGCCGCGGACATCGCCAAGCTGTTTCAGTCGGTGACCAGCGCCGAAGCGAAAATC
GATGAGCGCGGCTCGATCACGGTGGATGAGCGGACCAACAACATCATTGCCTACCAGACCCAGGACCGCCTCGACGAACT
GCGGCGGATCGTGGCGCAACTGGATATTCCGGTGCGCCAGGTGATGATCGAGGCACGGATCGTCGAGGCCAACGTCGATT
ACGACAAGAGCCTGGGCGTGCGCTGGGGCGGTTCGATCCAGAACAAGGGCAACTGGAACACGTCCGGGGTCAGTAACGGC
ACCTCGACCACCATCGGCACGCCGGGCAGCACCAGCACCAACTCGCCGTTCGTCGACATGGGCACGGTCGGCAACACGTC
GGGGATTGGTATCGCCTTCATCACCGACAATGTGCTGCTCGACCTTGAGCTGACGGCGATGGAAAAAACCGGCAACGGTG
AAATCGTCTCCCAGCCGAAAGTGGTCACCTCCGACAAGGAGACCGCGAAGATCCTCAAGGGCACCGAGATTCCGTATCAG
GAGGCCAGCTCCAGCGGCGCGACGTCAGTGTCTTTCAAGGAGGCCTCGCTGTCGCTCGAAGTGACGCCGCAGATCACCCC
CGACAACCGCATCATCATGGAGGTCAAGGTCACCAAGGATGAGCCGGATTACCTGAACAAGGTGCAGGATGTCCCGCCGA
TCAAGAAGAACGAGGTCAACGCCAAAGTGCTGGTGAACGACGGCGAGACCATCGTGATTGGGGGCGTTTTCTCAAATACT
CAAAGCAAGGTCGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATGTGGTTTC
GGAGAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(301-347)

Bacterial type II and III secretion system protein (Secretin)

(526-682)

Bacterial type II/III secretion system short domain (Secretin_N)

(375-440)

AMIN domain (AMIN)

(37-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.184

100

0.758
  comQ Acinetobacter baylyi ADP1

41.04

100

0.411
  pilQ Acinetobacter baumannii D1279779

37.769

100

0.407
  pilQ Acinetobacter baumannii strain A118

37.838

100

0.405
  pilQ Legionella pneumophila strain ERS1305867

38.542

97.25

0.375
  pilQ Legionella pneumophila strain Lp02

38.542

97.25

0.375

Multiple sequence alignment