Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   A8L59_RS02025 Genome accession   NZ_CP015852
Coordinates   456248..457312 (+) Length   354 a.a.
NCBI ID   WP_064585596.1    Uniprot ID   A0AAC9BP10
Organism   Pseudomonas koreensis strain CRS05-R5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 451248..462312
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A8L59_RS02015 (A8L59_02010) - 452105..453373 (+) 1269 WP_003220889.1 malic enzyme-like NAD(P)-binding protein -
  A8L59_RS02020 (A8L59_02015) - 453584..456028 (-) 2445 WP_373292956.1 penicillin-binding protein 1A -
  A8L59_RS02025 (A8L59_02020) pilM 456248..457312 (+) 1065 WP_064585596.1 pilus assembly protein PilM Machinery gene
  A8L59_RS02030 (A8L59_02025) - 457312..457878 (+) 567 WP_064585597.1 PilN domain-containing protein -
  A8L59_RS02035 (A8L59_02030) pilO 457875..458498 (+) 624 WP_064585598.1 type 4a pilus biogenesis protein PilO -
  A8L59_RS02040 (A8L59_02035) - 458495..459022 (+) 528 WP_064585599.1 pilus assembly protein PilP -
  A8L59_RS02045 (A8L59_02040) pilQ 459036..461111 (+) 2076 WP_064585600.1 type IV pilus secretin PilQ Machinery gene
  A8L59_RS02050 (A8L59_02045) aroK 461116..461634 (+) 519 WP_027611316.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38117.64 Da        Isoelectric Point: 4.8182

>NTDB_id=182491 A8L59_RS02025 WP_064585596.1 456248..457312(+) (pilM) [Pseudomonas koreensis strain CRS05-R5]
MLGLFNKKTNTLLGIDISSTSVKLLELSRQGDRYRVEAYAVEPLPPNAVVEKNIAELEGVGHALSRVLVKARTGLKSVAV
AVAGSAVITKVIEMDAGLTDDELENQLKIEADQYIPYPLDEVAIDFEVQGVSPRNPERVNVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLATQLAASQERLTVAVVDIGATMTTLSVLHNGRIIYTREQLFGGRQLTEEIQRRYGLTV
EQAGLAKKQGGLPDDYVSEVLQPFREALVQQVSRSLQFFFASGQYNAVDHILLAGGTASVPGLDRLIEQRLGTPTQVANP
FSDMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=182491 A8L59_RS02025 WP_064585596.1 456248..457312(+) (pilM) [Pseudomonas koreensis strain CRS05-R5]
GTGCTGGGACTCTTCAATAAAAAGACCAATACGTTACTGGGGATCGACATCAGCTCCACTTCGGTGAAGCTGCTGGAGCT
GAGCCGGCAGGGCGACCGCTACCGGGTCGAGGCCTACGCGGTGGAACCGCTGCCGCCCAACGCAGTCGTCGAAAAGAACA
TCGCCGAGCTCGAAGGCGTGGGCCATGCCTTGTCCCGAGTGTTGGTCAAGGCGCGCACCGGGCTCAAGAGCGTGGCGGTG
GCGGTGGCCGGTTCGGCGGTGATCACCAAGGTCATCGAGATGGACGCCGGGCTGACCGATGACGAGCTGGAAAACCAGCT
GAAGATTGAAGCCGATCAGTACATTCCCTATCCGCTGGACGAAGTCGCCATCGATTTCGAAGTCCAGGGCGTTTCGCCGC
GTAACCCCGAACGGGTCAATGTGCTGCTGGCCGCCTGCCGCAAGGAAAACGTCGAAGTCCGCGAGGCGGCGCTCGCGCTG
GCCGGGCTTACCGCGCGGGTGGTCGATGTCGAGGCCTACGCACTGGAGCGTTCGTTCGGCCTGCTCGCCACTCAGCTGGC
GGCCTCGCAGGAGCGCCTGACCGTAGCGGTGGTGGACATCGGCGCGACCATGACCACCCTCAGCGTCCTGCACAACGGCC
GCATCATCTATACCCGCGAGCAACTGTTCGGCGGGCGGCAACTGACCGAGGAAATCCAGCGTCGCTACGGCCTGACCGTC
GAACAGGCCGGACTGGCGAAAAAGCAGGGTGGTCTGCCCGACGACTACGTCAGCGAGGTGCTGCAGCCATTCCGGGAGGC
GCTGGTGCAGCAGGTGTCGCGCTCATTGCAGTTCTTCTTTGCCTCCGGGCAGTACAACGCGGTCGATCATATTCTGCTCG
CCGGTGGCACGGCGTCGGTGCCGGGGCTGGATCGCCTGATCGAGCAGCGTCTGGGCACTCCGACCCAGGTTGCCAACCCG
TTTTCCGACATGGCCTTGAGCAGCAAGGTCAACGCCGGGGCCCTGGCCAGTGACGCGCCGGCCCTGATGATTGCCTGCGG
GCTCGCGCTCAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.215

100

0.562
  comM Acinetobacter nosocomialis M2

56.215

100

0.562
  comM Acinetobacter baylyi ADP1

55.085

100

0.551
  pilM Legionella pneumophila strain ERS1305867

48.305

100

0.483

Multiple sequence alignment