Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   A8C75_RS05340 Genome accession   NZ_CP015839
Coordinates   1218392..1220101 (-) Length   569 a.a.
NCBI ID   WP_067379145.1    Uniprot ID   -
Organism   Marinobacterium aestuarii strain ST58-10     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1213392..1225101
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A8C75_RS05315 (A8C75_05315) - 1214320..1215273 (+) 954 WP_067379130.1 Nudix family hydrolase -
  A8C75_RS05320 (A8C75_05320) yacG 1215286..1215528 (-) 243 WP_067379133.1 DNA gyrase inhibitor YacG -
  A8C75_RS05325 (A8C75_05325) coaE 1215525..1216133 (-) 609 WP_067379136.1 dephospho-CoA kinase -
  A8C75_RS05330 (A8C75_05330) pilD 1216172..1217050 (-) 879 WP_067379139.1 prepilin peptidase Machinery gene
  A8C75_RS05335 (A8C75_05335) pilC 1217144..1218367 (-) 1224 WP_067379142.1 type II secretion system F family protein Machinery gene
  A8C75_RS05340 (A8C75_05340) pilB 1218392..1220101 (-) 1710 WP_067379145.1 type IV-A pilus assembly ATPase PilB Machinery gene
  A8C75_RS05345 (A8C75_05345) - 1220435..1220950 (+) 516 WP_157890226.1 pilin -
  A8C75_RS05350 (A8C75_05350) - 1221271..1221615 (+) 345 WP_067379148.1 hypothetical protein -
  A8C75_RS05355 (A8C75_05355) nadC 1221840..1222679 (-) 840 WP_067379151.1 carboxylating nicotinate-nucleotide diphosphorylase -
  A8C75_RS05360 (A8C75_05360) ampD 1222773..1223357 (+) 585 WP_067379154.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  A8C75_RS05365 (A8C75_05365) - 1223354..1224166 (+) 813 WP_067379155.1 hypothetical protein -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 62355.64 Da        Isoelectric Point: 5.5624

>NTDB_id=182391 A8C75_RS05340 WP_067379145.1 1218392..1220101(-) (pilB) [Marinobacterium aestuarii strain ST58-10]
MNAFQPLSGLAKRLVNDGVFSAEVAVDAIAQARAAQKPFISHIIEQRLVSAHRAACAASDEFGLPLLDLDVISREALPQG
LVSEALISKHHALPLMKRENRLFIAIADPTNIQALDEIKFHTGTTTEGVIVEEDKLRRLIDAFLDNQHSALDDLADTDLD
HLDVGDRPEVDIAETAEEAANDAPIVRFVNKILLDAIKNGASDIHFEPYEKNYRIRSRIDGILQEVAKPPANLAPRLAAR
LKVMSRMDISERRMPQDGRIKMKISAKRAIDFRVNSLPTLWGEKIVLRILDPASAKMGVDALGFEPDQKEIFLATLSKPQ
GMILVTGPTGSGKTVTLYTGLNILNDAERNISTAEDPVEINMEGINQVHVNTKVGLTFAEALRAFLRQDPDIVMVGEIRD
LETAEIGIKAAQTGHMVLSTLHTNSSPETLTRLRNMGVPAFNIATSVTLIIAQRLARRLCESCKRPADIPETALLEAGFS
AEQLETTTLFEANSEGCSKCNRGYKGRVGIYEMLPMSNEVANVIMNNGNSLDILRVAQAEGFRTLRLSGLIKVGKGLTSL
EEMNRVIVT

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=182391 A8C75_RS05340 WP_067379145.1 1218392..1220101(-) (pilB) [Marinobacterium aestuarii strain ST58-10]
GTGAACGCTTTTCAACCTCTCAGCGGTCTCGCCAAGCGACTGGTAAATGACGGCGTGTTCTCAGCCGAAGTCGCGGTCGA
TGCCATTGCCCAGGCAAGGGCCGCGCAAAAGCCCTTTATCAGCCATATTATTGAGCAGCGCCTGGTAAGTGCACACCGGG
CGGCCTGCGCCGCATCCGATGAATTCGGCCTGCCATTGCTTGATCTGGATGTCATCAGCCGCGAAGCCCTGCCCCAGGGC
CTGGTATCCGAAGCCCTGATCAGCAAGCATCACGCACTGCCGTTAATGAAGCGTGAAAATCGGCTCTTTATTGCCATCGC
CGACCCCACCAATATCCAGGCGCTGGATGAGATAAAATTTCATACAGGCACCACAACCGAAGGCGTCATTGTCGAAGAAG
ACAAGCTGCGCCGGCTGATTGATGCCTTTCTCGACAACCAGCACAGCGCGCTGGACGATCTGGCGGATACCGACCTAGAC
CACCTGGATGTCGGTGATCGCCCGGAAGTCGACATTGCCGAAACGGCGGAAGAAGCCGCCAACGATGCCCCCATCGTGCG
CTTTGTTAACAAGATACTGCTGGATGCCATCAAGAATGGCGCATCCGATATCCACTTTGAGCCCTACGAGAAAAATTACC
GCATCCGCAGCCGCATTGACGGCATATTGCAGGAGGTTGCCAAACCTCCAGCCAACCTGGCACCTCGCCTGGCAGCACGC
CTGAAGGTTATGTCCCGCATGGATATCTCCGAGCGGCGCATGCCTCAGGATGGCCGCATCAAGATGAAGATCTCGGCCAA
GCGCGCCATCGACTTTCGTGTCAACAGCCTGCCAACCCTGTGGGGGGAAAAAATCGTACTGCGAATTCTGGATCCTGCCA
GTGCCAAGATGGGTGTCGATGCCCTGGGTTTCGAGCCTGACCAGAAGGAAATATTCCTGGCAACGCTGTCCAAGCCCCAG
GGCATGATCCTGGTGACAGGCCCCACCGGCAGCGGCAAGACGGTGACACTCTATACCGGCCTGAATATTCTGAATGACGC
AGAGCGCAACATCTCCACCGCCGAGGATCCGGTGGAGATCAATATGGAGGGCATCAACCAGGTTCATGTGAACACCAAGG
TTGGCCTCACCTTCGCCGAAGCACTGCGCGCCTTTTTGCGCCAGGATCCCGATATTGTCATGGTCGGCGAGATACGTGAC
CTGGAAACCGCCGAGATTGGTATCAAGGCCGCCCAAACCGGCCATATGGTGCTCTCTACCCTGCACACCAACAGCTCTCC
CGAAACCCTGACGCGCCTGCGCAACATGGGCGTGCCTGCGTTCAACATCGCGACCTCGGTCACGCTGATCATTGCCCAGC
GACTGGCGCGGCGACTGTGCGAATCCTGCAAGCGACCAGCAGACATTCCCGAGACCGCCCTGCTGGAAGCAGGCTTTAGC
GCCGAACAATTGGAAACGACTACACTGTTTGAAGCCAACTCCGAGGGCTGCTCCAAATGCAACCGGGGCTACAAGGGCCG
GGTCGGGATTTACGAAATGCTGCCGATGAGTAACGAAGTGGCCAACGTCATCATGAACAACGGCAACTCACTCGATATTC
TTCGTGTCGCCCAGGCCGAGGGGTTTCGCACTCTGCGACTGTCCGGGCTGATCAAGGTTGGCAAGGGCCTTACCAGCCTG
GAAGAAATGAACCGGGTTATCGTTACCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-149)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.576

98.594

0.568
  pilB Acinetobacter baylyi ADP1

56.567

100

0.568
  pilB Legionella pneumophila strain ERS1305867

55.654

99.473

0.554
  pilB Vibrio cholerae strain A1552

49.912

100

0.499
  pilB Vibrio parahaemolyticus RIMD 2210633

48.85

99.297

0.485
  pilB Vibrio campbellii strain DS40M4

47.795

99.649

0.476
  pilF Neisseria gonorrhoeae MS11

48.301

98.243

0.475
  pilB/pilB1 Synechocystis sp. PCC 6803

41.085

90.685

0.373

Multiple sequence alignment