Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   A8C75_RS05330 Genome accession   NZ_CP015839
Coordinates   1216172..1217050 (-) Length   292 a.a.
NCBI ID   WP_067379139.1    Uniprot ID   A0A1A9EUX5
Organism   Marinobacterium aestuarii strain ST58-10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1211172..1222050
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A8C75_RS05310 (A8C75_05310) argJ 1213013..1214227 (+) 1215 WP_067379127.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -
  A8C75_RS05315 (A8C75_05315) - 1214320..1215273 (+) 954 WP_067379130.1 Nudix family hydrolase -
  A8C75_RS05320 (A8C75_05320) yacG 1215286..1215528 (-) 243 WP_067379133.1 DNA gyrase inhibitor YacG -
  A8C75_RS05325 (A8C75_05325) coaE 1215525..1216133 (-) 609 WP_067379136.1 dephospho-CoA kinase -
  A8C75_RS05330 (A8C75_05330) pilD 1216172..1217050 (-) 879 WP_067379139.1 prepilin peptidase Machinery gene
  A8C75_RS05335 (A8C75_05335) pilC 1217144..1218367 (-) 1224 WP_067379142.1 type II secretion system F family protein Machinery gene
  A8C75_RS05340 (A8C75_05340) pilB 1218392..1220101 (-) 1710 WP_067379145.1 type IV-A pilus assembly ATPase PilB Machinery gene
  A8C75_RS05345 (A8C75_05345) - 1220435..1220950 (+) 516 WP_157890226.1 pilin -
  A8C75_RS05350 (A8C75_05350) - 1221271..1221615 (+) 345 WP_067379148.1 hypothetical protein -

Sequence

Protein


Download         Length: 292 a.a.        Molecular weight: 32508.72 Da        Isoelectric Point: 6.5906

>NTDB_id=182389 A8C75_RS05330 WP_067379139.1 1216172..1217050(-) (pilD) [Marinobacterium aestuarii strain ST58-10]
MQIELFNTQGLELTLLGVTLAFVFSLLVGSFLNVVIHRLPLMMEREWQAMAAGADCSAEETSASSFNLATPASRCPHCQH
HIRWYENIPLISYMALLGKCSACSAPISLRYPVVELLTALISTFVLWHFGFNSVGYSLVALSWALITLTFIDIDHQLLPD
RITLPLIWLGLILNSFTLFTTLESALWGAVIGYLALWSIYWLFKLMTGKEGMGYGDFKLLAALGAWCGVEQLPLIILMSS
VVGILLAIVLMLLRKHQMANPLPFGPYLAIAGWVAIIWGRPITEAYLQLFRL

Nucleotide


Download         Length: 879 bp        

>NTDB_id=182389 A8C75_RS05330 WP_067379139.1 1216172..1217050(-) (pilD) [Marinobacterium aestuarii strain ST58-10]
ATGCAGATTGAGTTATTCAATACACAGGGCCTGGAGCTTACGCTTCTCGGGGTCACACTGGCGTTTGTATTTTCACTGCT
GGTCGGCAGTTTCCTTAATGTCGTTATCCATCGCCTGCCCCTGATGATGGAGCGTGAATGGCAGGCCATGGCGGCAGGCG
CCGACTGTAGCGCCGAGGAAACGAGCGCTAGCAGCTTTAATCTGGCGACTCCCGCATCCCGCTGCCCCCACTGCCAGCAC
CATATCCGCTGGTATGAAAATATTCCACTGATCAGCTATATGGCGCTGCTGGGCAAATGCTCCGCCTGCTCGGCGCCGAT
CTCGCTACGTTACCCGGTGGTCGAACTGCTAACCGCCCTGATCAGCACCTTTGTGCTCTGGCACTTCGGCTTTAATTCCG
TCGGCTACAGTCTGGTGGCACTGAGCTGGGCCCTGATCACTCTGACGTTTATCGATATTGACCACCAGTTGCTGCCGGAC
CGCATCACCCTGCCACTCATCTGGCTGGGGCTGATCCTCAACAGCTTTACACTCTTCACAACACTGGAATCGGCCCTCTG
GGGCGCGGTGATTGGCTACCTCGCGCTCTGGTCTATCTACTGGCTGTTCAAGCTCATGACCGGCAAGGAAGGCATGGGCT
ATGGCGACTTCAAGCTGCTGGCGGCACTCGGCGCCTGGTGCGGCGTGGAACAGCTGCCACTGATTATTCTGATGTCCTCG
GTAGTAGGCATCCTGCTGGCCATAGTGCTGATGCTGCTGCGCAAGCACCAGATGGCCAACCCATTGCCCTTTGGCCCCTA
CCTCGCGATTGCCGGCTGGGTCGCCATCATCTGGGGCCGCCCTATCACCGAAGCCTACCTGCAACTATTCAGGCTCTAA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(23-129)

Type IV leader peptidase family (Peptidase_A24)

(140-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1A9EUX5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Acinetobacter nosocomialis M2

53.43

94.863

0.507
  pilD Acinetobacter baumannii D1279779

53.43

94.863

0.507
  pilD Vibrio cholerae strain A1552

53.875

92.808

0.5
  pilD Vibrio campbellii strain DS40M4

51.838

93.151

0.483
  pilD Neisseria gonorrhoeae MS11

48.289

90.068

0.435

Multiple sequence alignment