Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   Eco28_RS22425 Genome accession   NZ_CP019944
Coordinates   4386329..4387714 (+) Length   461 a.a.
NCBI ID   WP_001025192.1    Uniprot ID   -
Organism   Escherichia coli strain DSM 103246     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4381329..4392714
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Eco28_RS22395 (Eco28_04311) - 4382313..4383263 (+) 951 WP_001154423.1 family 43 glycosylhydrolase -
  Eco28_RS22400 (Eco28_04312) ampE 4383293..4384147 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  Eco28_RS22405 (Eco28_04313) ampD 4384144..4384695 (-) 552 WP_000923722.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  Eco28_RS22410 (Eco28_04314) nadC 4384783..4385676 (+) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  Eco28_RS22420 (Eco28_04315) ppdD 4385879..4386319 (+) 441 WP_000360900.1 prepilin peptidase-dependent pilin -
  Eco28_RS22425 (Eco28_04316) pilB 4386329..4387714 (+) 1386 WP_001025192.1 type II secretion system protein GspE Machinery gene
  Eco28_RS22430 (Eco28_04317) hofC 4387704..4388906 (+) 1203 WP_000157263.1 protein transport protein HofC -
  Eco28_RS22435 (Eco28_04318) guaC 4388941..4389984 (-) 1044 WP_021576953.1 GMP reductase -
  Eco28_RS28500 - 4390140..4390184 (-) 45 WP_120795372.1 protein YacM -
  Eco28_RS22440 (Eco28_04319) coaE 4390209..4390829 (+) 621 WP_001269520.1 dephospho-CoA kinase -
  Eco28_RS22445 (Eco28_04320) zapD 4390829..4391572 (+) 744 WP_001194734.1 cell division protein ZapD -
  Eco28_RS22450 (Eco28_04321) yacG 4391582..4391779 (+) 198 WP_000005042.1 DNA gyrase inhibitor YacG -
  Eco28_RS22455 (Eco28_04322) mutT 4391995..4392384 (-) 390 WP_021576952.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 50581.22 Da        Isoelectric Point: 6.4542

>NTDB_id=182360 Eco28_RS22425 WP_001025192.1 4386329..4387714(+) (pilB) [Escherichia coli strain DSM 103246]
MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFATTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQP
KAELLTRTLQSALEQRASDIHIEPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLVLVTGPTGSGKTVTLYSALQTLN
TADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGVLRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNS
TCETLVRLQQMGVARWMLSSALTLVIAQRLVRKLCPHCRQQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGFYGRTALFE
VLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEELIRVLGMPHGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=182360 Eco28_RS22425 WP_001025192.1 4386329..4387714(+) (pilB) [Escherichia coli strain DSM 103246]
ATGAATATTCCACAGCTCACGGCCCTGTGTCTGCGTTATCAGGGAGTCTTGCTGGATGCCAGCGAAGAAGTGGTTCATGT
TGCGGTGGTCGATGCCCCCTCACATGAGTTGCTGGACGCATTGCATTTCGCTACCACCAAACGTATTGAGATCACCTGCT
GGACGCGCCAACAAATGGAAGGTCACGCCAGTCGCACACAACAGACATTGCCCGTAGCTGTTCAGGAGAAGCATCAGCCC
AAAGCAGAGTTGCTGACTCGAACGTTACAATCTGCGCTGGAACAACGCGCGTCTGATATTCATATCGAACCAGCGGACAA
TGCCTACCGCATCCGCTTGCGTATCGACGGCGTATTGCATCCTTTACCGGATGTTTCACCGGATGCCGGAGTCGCATTAA
CCGCCAGATTAAAAGTGCTGGGAAACCTGGATATTGCGGAACATCGCCTGCCGCAGGACGGGCAATTCACTGTCGAACTG
GCAGGAAACGCCGTCTCATTTCGTATTGCGACCTTACCATGTCGGGGTGGTGAAAAGGTGGTATTAAGGTTGTTACAGCA
GGTGAGCCAGGCACTGGATGTCAACACGCTGGGAATGCAGCCGTTACAACTGGCGGACTTTGCTCATGCCTTGCAACAAC
CACAGGGACTGGTGCTGGTAACTGGCCCTACCGGCAGCGGCAAAACGGTCACGCTTTATAGTGCCCTGCAAACGCTGAAT
ACCGCTGACATTAATATTTGTAGCGTCGAAGATCCGGTTGAGATCCCCATAGCCGGACTAAACCAGACGCAAATCCATCC
GCGTGCCGGACTCACCTTTCAGGGCGTTTTGCGTGCGTTATTGCGCCAGGATCCTGACGTCATCATGATCGGAGAGATCC
GCGATGGCGAAACGGCAGAGATCGCTATTAAAGCCGCGCAAACCGGTCACCTGGTGTTGTCTACCCTACACACCAATTCC
ACCTGCGAAACGCTGGTACGTTTACAGCAAATGGGAGTCGCCCGCTGGATGCTCTCATCAGCGCTTACGCTGGTAATAGC
CCAGCGTCTGGTACGTAAACTTTGCCCACATTGTCGCCAGCAGCAAGGGGAGCCCATCCATATTCCAGACAATGTATGGC
CGTCGCCGCTGCCCCACTGGCAGGCACCTGGTTGTGTACATTGCTACCACGGTTTTTATGGTCGCACGGCCTTATTTGAA
GTTCTGCCCATAACACCGGTCATACGTCAGCTTATTTCCGCTAATACCGACGTTGAATCGCTGGAAACTCACGCCCGACA
GGCGGGTATGCGTACGCTTTTTGAAAACGGCTGCCTCGCCGTGGAGCAAGGCTTAACCACCTTTGAAGAGTTAATCCGCG
TACTGGGGATGCCGCATGGCGAGTAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(8-68)

Type II/IV secretion system protein (T2SSE)

(88-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

50

83.297

0.416
  pilB Glaesserella parasuis strain SC1401

41.253

100

0.414
  pilB Vibrio campbellii strain DS40M4

48.705

83.731

0.408
  pilB Acinetobacter baylyi ADP1

39.957

100

0.406
  pilB Vibrio cholerae strain A1552

46.667

84.599

0.395
  pilB Vibrio parahaemolyticus RIMD 2210633

46.667

84.599

0.395
  pilB Haemophilus influenzae 86-028NP

45.22

83.948

0.38
  pilB Acinetobacter baumannii D1279779

43.909

85.466

0.375
  pilF Neisseria gonorrhoeae MS11

44.675

83.514

0.373
  pilB Haemophilus influenzae Rd KW20

44.444

83.948

0.373
  pilF Thermus thermophilus HB27

40.587

88.72

0.36