Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   B0915_RS00545 Genome accession   NZ_CP019778
Coordinates   120110..121495 (-) Length   461 a.a.
NCBI ID   WP_066009445.1    Uniprot ID   -
Organism   Escherichia coli strain NCTC86     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 115110..126495
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B0915_RS00515 (B0915_00535) mutT 115439..115829 (+) 391 Protein_101 8-oxo-dGTP diphosphatase MutT -
  B0915_RS00520 (B0915_00540) yacG 116045..116242 (-) 198 WP_000005042.1 DNA gyrase inhibitor YacG -
  B0915_RS00525 (B0915_00545) zapD 116252..116995 (-) 744 WP_001194734.1 cell division protein ZapD -
  B0915_RS00530 (B0915_00550) coaE 116995..117615 (-) 621 WP_001269520.1 dephospho-CoA kinase -
  B0915_RS26815 - 117640..117684 (+) 45 WP_120795372.1 protein YacM -
  B0915_RS00535 (B0915_00555) guaC 117840..118883 (+) 1044 WP_078045876.1 GMP reductase -
  B0915_RS00540 (B0915_00560) hofC 118918..120120 (-) 1203 WP_000157266.1 protein transport protein HofC -
  B0915_RS00545 (B0915_00565) pilB 120110..121495 (-) 1386 WP_066009445.1 type II secretion system protein GspE Machinery gene
  B0915_RS00550 (B0915_00570) pilA 121505..121945 (-) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  B0915_RS00560 (B0915_00580) nadC 122148..123041 (-) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  B0915_RS00565 (B0915_00585) ampD 123129..123680 (+) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  B0915_RS00570 (B0915_00590) ampE 123677..124531 (+) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  B0915_RS00575 (B0915_00595) aroP 124574..125947 (-) 1374 WP_000969915.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 50572.30 Da        Isoelectric Point: 6.5786

>NTDB_id=181835 B0915_RS00545 WP_066009445.1 120110..121495(-) (pilB) [Escherichia coli strain NCTC86]
MNIPQLTALCLRYHGVLLDASEEVVHVAVVDAPSHELLDALHFATTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQP
KAELLTRTLQSALEQRASDIHIEPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
AGNAVSFRIATLPCRGGEKVVLRLLQQVGQALDVNTLGMQPLQLADFAHALQQPQGLVLVTGPTGSGKTVTLYSALQKLN
TADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGVLRALLRQDPDVIMIGEILDGETAEIAIKAAQTGHLVLSTLHTNS
TCETLVRLQQMGVARWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGFYGRTALFE
VLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEELIRVLGMPHGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=181835 B0915_RS00545 WP_066009445.1 120110..121495(-) (pilB) [Escherichia coli strain NCTC86]
ATGAATATTCCACAGCTCACTGCCCTGTGTCTGCGTTATCATGGAGTCTTGCTGGATGCCAGCGAAGAGGTGGTTCATGT
TGCGGTAGTCGATGCACCTTCGCATGAGCTACTGGACGCATTGCATTTCGCTACCACCAAACGTATTGAGATCACCTGCT
GGACGCGCCAACAAATGGAAGGTCACGCCAGTCGCACACAACAGACATTGCCCGTAGCTGTTCAGGAGAAGCATCAGCCC
AAAGCAGAGTTGCTAACTCGAACGTTACAATCTGCGCTGGAACAACGCGCGTCTGATATTCATATCGAACCAGCGGACAA
TGCCTACCGCATCCGCTTGCGTATCGACGGCGTATTGCATCCTTTACCGGATGTTTCACCGGATGCCGGAGTCGCATTAA
CCGCCAGATTAAAAGTGCTGGGAAACCTGGATATTGCGGAACATCGCCTGCCGCAGGACGGGCAATTCACTGTCGAACTG
GCAGGAAACGCCGTCTCATTTCGTATTGCGACCTTACCATGTCGGGGTGGTGAAAAGGTGGTATTAAGGTTGTTACAGCA
GGTGGGTCAGGCACTGGATGTCAACACGCTTGGAATGCAGCCGTTACAACTGGCGGACTTTGCTCATGCCTTGCAACAAC
CACAGGGACTGGTGCTGGTAACTGGCCCTACCGGCAGCGGCAAAACGGTCACGCTTTATAGTGCCCTGCAAAAGCTGAAT
ACCGCTGACATTAATATTTGTAGCGTCGAAGATCCAGTTGAGATCCCCATAGCCGGACTAAACCAGACGCAAATCCATCC
GCGTGCCGGACTCACCTTTCAGGGCGTTTTGCGTGCGTTATTGCGCCAGGATCCTGACGTCATCATGATCGGAGAGATCC
TCGATGGCGAAACAGCAGAGATCGCTATTAAAGCGGCGCAAACTGGTCACCTGGTGTTGTCTACCCTACACACTAATTCC
ACCTGCGAAACGCTGGTACGTTTACAGCAAATGGGGGTCGCCCGCTGGATGCTATCATCGGCGCTTACGCTGGTAATAGC
CCAGCGTCTGGTACGCAAACTTTGCCCACATTGTCGCCGGCAGCAAGGGGAGCCCATCCACATTCCAGACAATGTATGGC
CATCGCCGCTGCCCCACTGGCAGGCACCCGGTTGTGTACATTGCTACCACGGTTTTTATGGTCGTACGGCCTTATTTGAA
GTTCTGCCCATAACGCCGGTCATTCGTCAGCTTATTTCCGCTAATACCGACGTTGAATCGCTGGAAACGCACGCACGACA
GGCGGGTATGCGTACGCTTTTTGAAAACGGCTGCCTGGCCGTAGAGCAAGGCTTAACCACCTTTGAAGAGTTAATCCGCG
TACTGGGGATGCCGCATGGCGAGTAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(8-68)

Type II/IV secretion system protein (T2SSE)

(88-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

42.236

100

0.443
  pilB Glaesserella parasuis strain SC1401

41.037

100

0.412
  pilB Legionella pneumophila strain ERS1305867

49.476

82.863

0.41
  pilB Acinetobacter baylyi ADP1

39.744

100

0.403
  pilB Vibrio cholerae strain A1552

46.41

84.599

0.393
  pilB Vibrio parahaemolyticus RIMD 2210633

45.897

84.599

0.388
  pilB Haemophilus influenzae 86-028NP

44.961

83.948

0.377
  pilB Acinetobacter baumannii D1279779

43.401

85.466

0.371
  pilB Haemophilus influenzae Rd KW20

44.186

83.948

0.371
  pilF Neisseria gonorrhoeae MS11

44.156

83.514

0.369

Multiple sequence alignment