Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   B0915_RS00550 Genome accession   NZ_CP019778
Coordinates   121505..121945 (-) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain NCTC86     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 116505..126945
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B0915_RS00530 (B0915_00550) coaE 116995..117615 (-) 621 WP_001269520.1 dephospho-CoA kinase -
  B0915_RS26815 - 117640..117684 (+) 45 WP_120795372.1 protein YacM -
  B0915_RS00535 (B0915_00555) guaC 117840..118883 (+) 1044 WP_078045876.1 GMP reductase -
  B0915_RS00540 (B0915_00560) hofC 118918..120120 (-) 1203 WP_000157266.1 protein transport protein HofC -
  B0915_RS00545 (B0915_00565) pilB 120110..121495 (-) 1386 WP_066009445.1 type II secretion system protein GspE Machinery gene
  B0915_RS00550 (B0915_00570) pilA 121505..121945 (-) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  B0915_RS00560 (B0915_00580) nadC 122148..123041 (-) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  B0915_RS00565 (B0915_00585) ampD 123129..123680 (+) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  B0915_RS00570 (B0915_00590) ampE 123677..124531 (+) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  B0915_RS00575 (B0915_00595) aroP 124574..125947 (-) 1374 WP_000969915.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=181836 B0915_RS00550 WP_000360904.1 121505..121945(-) (pilA) [Escherichia coli strain NCTC86]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=181836 B0915_RS00550 WP_000360904.1 121505..121945(-) (pilA) [Escherichia coli strain NCTC86]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363