Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BXO92_RS13955 Genome accession   NZ_CP019455
Coordinates   2791842..2793227 (-) Length   461 a.a.
NCBI ID   WP_001025178.1    Uniprot ID   A0A0H2YVM6
Organism   Escherichia coli strain FHI_NMBU_03     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2786842..2798227
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BXO92_RS13925 (BXO92_14140) mutT 2787291..2787689 (+) 399 WP_000736044.1 8-oxo-dGTP diphosphatase MutT -
  BXO92_RS13930 (BXO92_14145) yacG 2787777..2787974 (-) 198 WP_000005042.1 DNA gyrase inhibitor YacG -
  BXO92_RS13935 (BXO92_14150) zapD 2787984..2788727 (-) 744 WP_001194731.1 cell division protein ZapD -
  BXO92_RS13940 (BXO92_14155) coaE 2788727..2789347 (-) 621 WP_001269524.1 dephospho-CoA kinase -
  BXO92_RS25085 - 2789372..2789416 (+) 45 WP_120795372.1 protein YacM -
  BXO92_RS13945 (BXO92_14160) guaC 2789572..2790615 (+) 1044 WP_001217338.1 GMP reductase -
  BXO92_RS13950 (BXO92_14165) hofC 2790650..2791852 (-) 1203 WP_024223366.1 protein transport protein HofC -
  BXO92_RS13955 (BXO92_14170) pilB 2791842..2793227 (-) 1386 WP_001025178.1 type II secretion system protein GspE Machinery gene
  BXO92_RS13960 (BXO92_14175) pilA 2793237..2793677 (-) 441 WP_024223365.1 prepilin peptidase-dependent pilin Machinery gene
  BXO92_RS13970 (BXO92_14185) nadC 2793881..2794774 (-) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  BXO92_RS13975 (BXO92_14190) ampD 2794862..2795413 (+) 552 WP_000923726.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  BXO92_RS13980 (BXO92_14195) ampE 2795410..2796264 (+) 855 WP_000172010.1 beta-lactamase regulator AmpE -
  BXO92_RS13985 (BXO92_14200) aroP 2796307..2797677 (-) 1371 WP_000399017.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 50632.29 Da        Isoelectric Point: 6.5396

>NTDB_id=180572 BXO92_RS13955 WP_001025178.1 2791842..2793227(-) (pilB) [Escherichia coli strain FHI_NMBU_03]
MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFATTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQP
KAELLTRTLQSALEQRASDIHIEPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
AGNAVSFRIATLACRGGEKVVLRLLQQVNQALDVNTLGMQPSQLVDFAHALQQPQGLVLVTGPTGSGKTVTLYSALQMLN
TADINICSVEDPVEIPIAGLNQTQIHSRAGLTFQGVLRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNS
TCETLVRLQQMGVARWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGFYGRTALFE
VLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEELIRVLGMPHGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=180572 BXO92_RS13955 WP_001025178.1 2791842..2793227(-) (pilB) [Escherichia coli strain FHI_NMBU_03]
ATGAATATTCCACAGCTCACCGCCCTGTGTCTGCGTTATCAGGGAGTCTTGCTGGATGCCAGCGAAGAGGTGGTTCATGT
TGCGGTAGTCGATGCACCTTCGCATGAGCTACTGGACGCATTGCATTTCGCTACCACCAAACGTATTGAGATCACCTGCT
GGACGCGCCAACAAATGGAAGGTCACGCCAGTCGCACACAACAGACATTGCCCGTAGCTGTTCAGGAGAAGCATCAGCCC
AAAGCAGAGTTGCTGACTCGAACGTTACAATCTGCGCTGGAACAACGTGCGTCTGATATTCATATCGAACCAGCGGACAA
TGCCTACCGCATCCGCTTGCGTATCGACGGCGTATTGCATCCTTTACCGGATGTTTCACCGGATGCCGGAGTCGCATTAA
CCGCCAGATTAAAAGTGCTGGGAAACCTGGATATTGCGGAACATCGCCTGCCGCAGGACGGGCAATTCACTGTCGAACTG
GCAGGAAACGCCGTCTCATTTCGTATTGCGACCTTAGCATGTCGGGGTGGTGAAAAGGTGGTATTAAGGTTGTTACAGCA
GGTGAACCAGGCACTGGATGTTAACACGCTTGGAATGCAGCCGTCACAACTGGTGGACTTTGCTCATGCCTTGCAACAAC
CACAGGGACTGGTGCTGGTAACTGGCCCTACAGGCAGCGGCAAAACGGTCACGCTTTATAGTGCCCTGCAAATGCTGAAT
ACCGCTGACATTAATATTTGTAGCGTCGAAGATCCGGTTGAGATCCCCATAGCCGGACTAAACCAGACGCAAATCCATTC
GCGTGCCGGACTCACCTTTCAGGGCGTTTTGCGTGCGTTATTGCGCCAGGATCCTGACGTCATCATGATCGGAGAGATCC
GCGATGGCGAAACGGCAGAAATTGCCATTAAAGCCGCGCAAACCGGTCACCTGGTGTTGTCTACCCTACACACTAATTCC
ACCTGCGAAACGCTGGTACGTTTACAGCAAATGGGAGTCGCCCGCTGGATGCTCTCATCGGCGCTTACGCTGGTAATAGC
CCAGCGTCTGGTACGCAAACTTTGCCCGCATTGTCGCCGGCAGCAAGGGGAGCCCATCCATATTCCAGACAATGTATGGC
CGTCGCCGCTGCCCCACTGGCAGGCACCCGGTTGTGTACATTGCTACCACGGTTTTTATGGTCGCACGGCCTTATTTGAA
GTTCTGCCCATAACACCGGTCATACGCCAGCTTATTTCCGCTAATACCGACGTTGAATCGCTGGAAACGCACGCCCGACA
GGCGGGTATGCGAACGCTTTTTGAAAACGGCTGCCTGGCCGTGGAGCAAGGCTTAACCACCTTTGAAGAGTTAATCCGCG
TATTGGGGATGCCGCATGGCGAGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(88-353)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(8-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2YVM6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

41.037

100

0.412
  pilB Legionella pneumophila strain ERS1305867

49.479

83.297

0.412
  pilB Acinetobacter baylyi ADP1

39.53

100

0.401
  pilB Vibrio campbellii strain DS40M4

47.927

83.731

0.401
  pilB Vibrio cholerae strain A1552

46.154

84.599

0.39
  pilB Vibrio parahaemolyticus RIMD 2210633

45.641

84.599

0.386
  pilB Haemophilus influenzae 86-028NP

44.703

83.948

0.375
  pilB Haemophilus influenzae Rd KW20

43.928

83.948

0.369
  pilF Neisseria gonorrhoeae MS11

44.156

83.514

0.369
  pilB Acinetobacter baumannii D1279779

42.893

85.466

0.367