Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   BXO92_RS13960 Genome accession   NZ_CP019455
Coordinates   2793237..2793677 (-) Length   146 a.a.
NCBI ID   WP_024223365.1    Uniprot ID   -
Organism   Escherichia coli strain FHI_NMBU_03     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2788237..2798677
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BXO92_RS13940 (BXO92_14155) coaE 2788727..2789347 (-) 621 WP_001269524.1 dephospho-CoA kinase -
  BXO92_RS25085 - 2789372..2789416 (+) 45 WP_120795372.1 protein YacM -
  BXO92_RS13945 (BXO92_14160) guaC 2789572..2790615 (+) 1044 WP_001217338.1 GMP reductase -
  BXO92_RS13950 (BXO92_14165) hofC 2790650..2791852 (-) 1203 WP_024223366.1 protein transport protein HofC -
  BXO92_RS13955 (BXO92_14170) pilB 2791842..2793227 (-) 1386 WP_001025178.1 type II secretion system protein GspE Machinery gene
  BXO92_RS13960 (BXO92_14175) pilA 2793237..2793677 (-) 441 WP_024223365.1 prepilin peptidase-dependent pilin Machinery gene
  BXO92_RS13970 (BXO92_14185) nadC 2793881..2794774 (-) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  BXO92_RS13975 (BXO92_14190) ampD 2794862..2795413 (+) 552 WP_000923726.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  BXO92_RS13980 (BXO92_14195) ampE 2795410..2796264 (+) 855 WP_000172010.1 beta-lactamase regulator AmpE -
  BXO92_RS13985 (BXO92_14200) aroP 2796307..2797677 (-) 1371 WP_000399017.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15662.85 Da        Isoelectric Point: 4.5237

>NTDB_id=180573 BXO92_RS13960 WP_024223365.1 2793237..2793677(-) (pilA) [Escherichia coli strain FHI_NMBU_03]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTR
YVSAMSVVKGVVSLTGQESLNGLSIVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=180573 BXO92_RS13960 WP_024223365.1 2793237..2793677(-) (pilA) [Escherichia coli strain FHI_NMBU_03]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAAATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGTAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCATCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGTAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTTCGCTTTGATGACGCAAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363

Multiple sequence alignment