Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BWI82_RS20655 Genome accession   NZ_CP019243
Coordinates   4034629..4036014 (-) Length   461 a.a.
NCBI ID   WP_001025180.1    Uniprot ID   A0A0H2V435
Organism   Escherichia coli strain Combat2C1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4029629..4041014
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BWI82_RS20625 (BWI82_20670) mutT 4030079..4030477 (+) 399 WP_000736044.1 8-oxo-dGTP diphosphatase MutT -
  BWI82_RS20630 (BWI82_20675) yacG 4030565..4030762 (-) 198 WP_000005042.1 DNA gyrase inhibitor YacG -
  BWI82_RS20635 (BWI82_20680) zapD 4030772..4031515 (-) 744 WP_001194731.1 cell division protein ZapD -
  BWI82_RS20640 (BWI82_20685) coaE 4031515..4032135 (-) 621 WP_001269522.1 dephospho-CoA kinase -
  BWI82_RS27760 - 4032160..4032204 (+) 45 WP_120795372.1 protein YacM -
  BWI82_RS20645 (BWI82_20690) guaC 4032359..4033402 (+) 1044 WP_001217338.1 GMP reductase -
  BWI82_RS20650 (BWI82_20695) hofC 4033437..4034639 (-) 1203 WP_000157289.1 protein transport protein HofC -
  BWI82_RS20655 (BWI82_20700) pilB 4034629..4036014 (-) 1386 WP_001025180.1 type II secretion system protein GspE Machinery gene
  BWI82_RS20660 (BWI82_20705) pilA 4036024..4036464 (-) 441 WP_000360914.1 prepilin peptidase-dependent pilin Machinery gene
  BWI82_RS20670 (BWI82_20715) nadC 4036668..4037561 (-) 894 WP_001135161.1 carboxylating nicotinate-nucleotide diphosphorylase -
  BWI82_RS20675 (BWI82_20720) ampD 4037649..4038200 (+) 552 WP_000923703.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  BWI82_RS20680 (BWI82_20725) ampE 4038197..4039051 (+) 855 WP_000171984.1 beta-lactamase regulator AmpE -
  BWI82_RS20685 (BWI82_20730) aroP 4039094..4040464 (-) 1371 WP_000399019.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 50602.20 Da        Isoelectric Point: 6.5396

>NTDB_id=179062 BWI82_RS20655 WP_001025180.1 4034629..4036014(-) (pilB) [Escherichia coli strain Combat2C1]
MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFATTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQP
KAELLTRTLQSALEQRASDIHIEPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
AGNAVSFRIATLACRGGEKVVLRLLQQVNQALDVNTLGMQPSQLVDFAHALQQPQGLVLVTGPTGSGKTVTLYSALQTLN
TADINICSVEDPVEIPIAGLNQTQIHSRAGLTFQGVLRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNS
TCETLVRLQQMGVARWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGFYGRTALFE
VLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEELIRVLGMPHGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=179062 BWI82_RS20655 WP_001025180.1 4034629..4036014(-) (pilB) [Escherichia coli strain Combat2C1]
ATGAATATTCCACAGCTCACTGCCCTGTGTCTGCGTTATCAGGGAGTCTTGCTGGATGCCAGCGAAGAGGTGGTTCATGT
TGCGGTAGTCGATGCACCTTCGCATGAGCTACTGGACGCATTGCATTTCGCTACCACCAAACGTATTGAGATCACCTGCT
GGACACGCCAACAAATGGAAGGTCACGCCAGTCGCACACAACAGACATTGCCCGTAGCTGTTCAGGAGAAGCATCAGCCC
AAAGCAGAGTTGCTGACTCGAACGTTACAATCTGCGCTAGAACAACGCGCGTCTGATATTCATATCGAACCAGCGGACAA
TGCCTACCGCATCCGCTTGCGTATCGACGGCGTATTGCATCCTTTACCGGACGTTTCACCGGATGCCGGAGTCGCATTAA
CCGCCAGATTAAAAGTGCTGGGAAACCTGGATATTGCGGAACATCGCCTGCCGCAGGACGGGCAATTCACTGTCGAACTG
GCAGGAAACGCCGTCTCATTTCGTATTGCGACCTTAGCATGTCGGGGTGGTGAAAAGGTGGTATTAAGGTTGTTACAGCA
GGTGAACCAGGCACTGGATGTTAACACGCTTGGAATGCAGCCGTCACAACTGGTGGACTTTGCTCATGCCTTGCAACAAC
CACAGGGACTGGTGCTGGTAACTGGCCCTACAGGCAGCGGCAAAACGGTCACGCTTTATAGTGCCCTGCAAACGCTGAAT
ACCGCTGACATTAATATTTGTAGCGTCGAAGATCCGGTTGAGATCCCCATAGCCGGATTAAACCAGACGCAAATCCATTC
GCGTGCCGGACTCACCTTTCAGGGCGTTTTGCGTGCGTTATTGCGCCAGGATCCTGACGTCATCATGATCGGAGAGATCC
GCGATGGCGAAACGGCAGAAATTGCCATTAAAGCCGCGCAAACCGGTCACCTGGTGTTGTCTACCCTACACACTAACTCC
ACCTGCGAAACGCTGGTACGTTTACAGCAAATGGGAGTCGCCCGCTGGATGCTATCATCGGCGCTTACGCTGGTAATAGC
CCAGCGTCTGGTACGCAAACTTTGCCCGCATTGTCGCCGGCAGCAAGGGGAGCCCATCCATATTCCAGACAATGTATGGC
CATCGCCGCTGCCCCACTGGCAGGCACCCGGTTGTGTACATTGCTACCACGGTTTTTATGGTCGCACGGCCTTATTTGAA
GTTCTGCCCATAACACCGGTCATACGTCAGCTTATTTCCGCTAATACCGACGTTGAATCGCTGGAAACGCACGCCCGACA
GGCGGGTATGCGAACGCTTTTTGAAAACGGCTGCCTGGCCGTGGAGCAAGGCTTAACCACCTTTGAAGAGTTAATCCGCG
TATTGGGGATGCCGCATGGCGAGTAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(8-68)

Type II/IV secretion system protein (T2SSE)

(88-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2V435

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

41.037

100

0.412
  pilB Legionella pneumophila strain ERS1305867

49.479

83.297

0.412
  pilB Acinetobacter baylyi ADP1

39.53

100

0.401
  pilB Vibrio campbellii strain DS40M4

47.927

83.731

0.401
  pilB Vibrio cholerae strain A1552

46.154

84.599

0.39
  pilB Vibrio parahaemolyticus RIMD 2210633

45.641

84.599

0.386
  pilB Haemophilus influenzae 86-028NP

44.703

83.948

0.375
  pilB Haemophilus influenzae Rd KW20

43.928

83.948

0.369
  pilF Neisseria gonorrhoeae MS11

44.156

83.514

0.369
  pilB Acinetobacter baumannii D1279779

42.893

85.466

0.367