Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   BWI82_RS20660 Genome accession   NZ_CP019243
Coordinates   4036024..4036464 (-) Length   146 a.a.
NCBI ID   WP_000360914.1    Uniprot ID   A0A0H2YVZ3
Organism   Escherichia coli strain Combat2C1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4031024..4041464
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BWI82_RS20640 (BWI82_20685) coaE 4031515..4032135 (-) 621 WP_001269522.1 dephospho-CoA kinase -
  BWI82_RS27760 - 4032160..4032204 (+) 45 WP_120795372.1 protein YacM -
  BWI82_RS20645 (BWI82_20690) guaC 4032359..4033402 (+) 1044 WP_001217338.1 GMP reductase -
  BWI82_RS20650 (BWI82_20695) hofC 4033437..4034639 (-) 1203 WP_000157289.1 protein transport protein HofC -
  BWI82_RS20655 (BWI82_20700) pilB 4034629..4036014 (-) 1386 WP_001025180.1 type II secretion system protein GspE Machinery gene
  BWI82_RS20660 (BWI82_20705) pilA 4036024..4036464 (-) 441 WP_000360914.1 prepilin peptidase-dependent pilin Machinery gene
  BWI82_RS20670 (BWI82_20715) nadC 4036668..4037561 (-) 894 WP_001135161.1 carboxylating nicotinate-nucleotide diphosphorylase -
  BWI82_RS20675 (BWI82_20720) ampD 4037649..4038200 (+) 552 WP_000923703.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  BWI82_RS20680 (BWI82_20725) ampE 4038197..4039051 (+) 855 WP_000171984.1 beta-lactamase regulator AmpE -
  BWI82_RS20685 (BWI82_20730) aroP 4039094..4040464 (-) 1371 WP_000399019.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15620.77 Da        Isoelectric Point: 4.5237

>NTDB_id=179063 BWI82_RS20660 WP_000360914.1 4036024..4036464(-) (pilA) [Escherichia coli strain Combat2C1]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=179063 BWI82_RS20660 WP_000360914.1 4036024..4036464(-) (pilA) [Escherichia coli strain Combat2C1]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAAATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACGCCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGTAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2YVZ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363