Detailed information NTG0178045

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: I596_RS11845
• Genome accession: NZ_CP015249
• Coordinates: 2915670..2917385 (+)
• Length: 571 a.a.
• NCBI ID: WP_067651867.1
• Uniprot ID: -
• Organism: Dokdonella koreensis DS-123
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2910670..2922385

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I596_RS11835 (I596_2533)	tfpZ	2913282..2914022 (+)	741	WP_067648107.1	TfpX/TfpZ family type IV pilin accessory protein	-
I596_RS11840 (I596_2534)	-	2914492..2914962 (+)	471	WP_067648111.1	pilin	-
I596_RS18110	pilA/pilAI	2915093..2915482 (+)	390	WP_083965547.1	pilin	Machinery gene
I596_RS11845 (I596_2535)	pilB	2915670..2917385 (+)	1716	WP_067651867.1	type IV-A pilus assembly ATPase PilB	Machinery gene
I596_RS11850 (I596_2536)	pilC	2917389..2918681 (+)	1293	WP_067648114.1	type II secretion system F family protein	Machinery gene
I596_RS11855 (I596_2537)	pilD	2918681..2919547 (+)	867	WP_067648117.1	A24 family peptidase	Machinery gene
I596_RS11860 (I596_2538)	-	2919678..2920829 (-)	1152	WP_083965548.1	efflux RND transporter periplasmic adaptor subunit	-

Sequence

Protein

• Length: 571 a.a.
• Molecular weight: 61613.88 Da
• Isoelectric Point: 6.1491

>NTDB_id=178045 I596_RS11845 WP_067651867.1 2915670..2917385(+) (pilB) [Dokdonella koreensis DS-123]
MNTAALAGLSGIARRLVADGILGENEARKAADEAARQRIPIGPWLIEHGLVGSGLVAQATSAEFGIPLIDLAGIDVTQVP
IKLVKEELVTKHRALPLFKRGNRLFLGVSDPTNLRALDEIKFQSNHSVEPILVDDALLDRAIDQALTATNATLESLGDDE
GLENLDLGGVDGDEAETGVDANSTEDAPVVRFINKVLVDAIKRGASDIHFEPYETAYRVRLRMDGMLRAVASPPVKLNSR
IASRLKVMSGLDIAEKRVPQDGRIKLNLSKTKSIDFRVSTCPTLFGEKIVLRILDAAAAKLGIDKLGYEEDQKKLFLEAI
EKPYGMVLVTGPTGSGKTVSLYTALNILNVEGRNISTVEDPVEIRVPGINQVQQNAKRGMTFAAALRSFLRQDPDVIMVG
EIRDLETAEIAIKAAQTGHMVLSTLHTNDGPQTISRLMNMGIAPYNITSSVTLVIAQRLARRLHDCKRPANIPAPALLAE
GFTQAEIDAGLDIFEAVGCNGCNEGYKGRLGIYQVMPMSEDIQKIVLAGGNAIQIATEAKRIGINDLRASALLKVKNGHT
SLAEINRVTKD

Nucleotide

• Length: 1716 bp

>NTDB_id=178045 I596_RS11845 WP_067651867.1 2915670..2917385(+) (pilB) [Dokdonella koreensis DS-123]
ATGAATACGGCGGCGCTGGCGGGCCTGAGCGGCATCGCCCGGCGTTTGGTGGCCGACGGCATCCTCGGCGAGAACGAAGC
GCGCAAGGCCGCCGACGAAGCGGCCCGCCAGCGCATCCCGATCGGCCCCTGGCTGATCGAGCACGGCCTGGTCGGTAGCG
GCCTGGTGGCCCAGGCGACCTCGGCTGAGTTTGGTATCCCCTTGATCGACCTTGCCGGCATCGACGTCACCCAGGTGCCG
ATCAAGCTGGTCAAGGAGGAACTGGTCACCAAGCACCGCGCCCTGCCGTTGTTCAAGCGCGGCAACCGATTGTTCCTTGG
TGTGTCGGACCCGACCAACCTGCGTGCGCTGGACGAGATCAAGTTCCAGTCCAACCACAGTGTGGAGCCGATCCTGGTGG
ACGATGCCCTGTTGGACCGGGCGATCGACCAGGCACTGACGGCCACCAACGCGACGCTGGAATCCCTGGGCGACGACGAG
GGTCTGGAGAACCTGGACCTCGGCGGTGTCGATGGAGACGAGGCCGAAACCGGCGTCGACGCCAACTCCACCGAGGACGC
CCCGGTGGTGCGCTTCATCAACAAGGTGCTGGTCGACGCGATCAAGCGCGGCGCCTCGGACATCCACTTCGAGCCCTACG
AGACCGCCTACCGCGTGCGCCTGCGCATGGATGGCATGTTGCGGGCGGTCGCCTCGCCACCCGTCAAGCTCAACTCACGC
ATCGCCTCGCGCCTGAAGGTCATGAGCGGCCTGGACATCGCCGAGAAACGGGTGCCGCAGGACGGGCGCATCAAGCTCAA
CCTGTCCAAGACCAAGTCGATCGACTTCCGCGTCAGCACCTGCCCCACGCTGTTCGGCGAGAAGATCGTGCTGCGTATTT
TGGATGCCGCGGCGGCCAAGCTCGGCATCGACAAGCTGGGCTACGAGGAAGACCAGAAGAAGCTGTTCCTGGAAGCGATC
GAGAAGCCCTACGGCATGGTGCTGGTCACCGGCCCGACCGGCTCGGGCAAAACGGTATCGCTCTATACGGCGCTCAACAT
CCTCAACGTGGAGGGGCGCAACATCTCCACGGTGGAGGACCCGGTCGAAATCCGTGTGCCGGGCATCAACCAGGTGCAGC
AGAACGCCAAGCGCGGCATGACGTTCGCCGCAGCGCTGCGCAGCTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGC
GAGATCCGCGACCTGGAAACCGCCGAGATTGCGATCAAGGCCGCGCAGACCGGCCATATGGTGCTCTCGACCCTGCACAC
CAATGACGGTCCGCAAACGATCTCGCGCCTGATGAACATGGGCATTGCGCCCTACAACATCACCAGTTCCGTGACGCTGG
TGATCGCCCAGCGGCTGGCGCGGCGCCTGCACGACTGCAAGCGGCCAGCCAACATCCCTGCACCCGCCCTGCTGGCCGAA
GGCTTTACCCAGGCCGAGATCGACGCAGGACTGGACATCTTTGAAGCGGTCGGCTGCAACGGCTGTAACGAGGGCTACAA
GGGCCGCCTGGGCATCTACCAGGTGATGCCGATGAGCGAGGACATCCAGAAGATCGTTCTCGCCGGTGGCAACGCCATAC
AGATTGCCACCGAGGCCAAGCGCATCGGCATCAACGACCTGCGTGCCTCGGCCTTGCTCAAGGTCAAGAACGGGCATACC
AGCCTCGCCGAAATCAATCGCGTCACGAAGGACTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (195-462)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (45-145)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baylyi ADP1	54.609	100	0.55
pilB	Acinetobacter baumannii D1279779	55.575	98.949	0.55
pilB	Legionella pneumophila strain ERS1305867	50.175	100	0.503
pilF	Neisseria gonorrhoeae MS11	48.85	98.949	0.483
pilB	Vibrio campbellii strain DS40M4	47.018	99.825	0.469
pilB	Vibrio parahaemolyticus RIMD 2210633	47.1	99.65	0.469
pilB	Vibrio cholerae strain A1552	50.857	91.944	0.468
pilF	Thermus thermophilus HB27	38.043	96.673	0.368
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	37.23	97.373	0.363