Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BE949_RS16410 Genome accession   NZ_CP018976
Coordinates   2865740..2867125 (+) Length   461 a.a.
NCBI ID   WP_001550607.1    Uniprot ID   -
Organism   Escherichia coli strain Ecol_545     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2860740..2872125
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BE949_RS16380 (BE949_16635) aroP 2861288..2862661 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  BE949_RS16385 (BE949_16640) ampE 2862704..2863558 (-) 855 WP_001550611.1 beta-lactamase regulator AmpE -
  BE949_RS16390 (BE949_16645) ampD 2863555..2864106 (-) 552 WP_001550610.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  BE949_RS16395 (BE949_16650) nadC 2864194..2865087 (+) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  BE949_RS16405 (BE949_16660) ppdD 2865291..2865730 (+) 440 Protein_2729 prepilin peptidase-dependent pilin -
  BE949_RS16410 (BE949_16665) pilB 2865740..2867125 (+) 1386 WP_001550607.1 type II secretion system protein GspE Machinery gene
  BE949_RS16415 (BE949_16670) hofC 2867115..2868316 (+) 1202 Protein_2731 protein transport protein HofC -
  BE949_RS16420 (BE949_16675) guaC 2868351..2869394 (-) 1044 WP_001217337.1 GMP reductase -
  BE949_RS27850 - 2869550..2869594 (-) 45 WP_120795372.1 protein YacM -
  BE949_RS16425 (BE949_16680) coaE 2869619..2870239 (+) 621 WP_001269520.1 dephospho-CoA kinase -
  BE949_RS16430 (BE949_16685) zapD 2870239..2870982 (+) 744 WP_001194736.1 cell division protein ZapD -
  BE949_RS16435 (BE949_16690) yacG 2870992..2871189 (+) 198 WP_000005042.1 DNA gyrase inhibitor YacG -
  BE949_RS16440 (BE949_16695) mutT 2871277..2871675 (-) 399 WP_001550604.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 50699.41 Da        Isoelectric Point: 6.6782

>NTDB_id=177367 BE949_RS16410 WP_001550607.1 2865740..2867125(+) (pilB) [Escherichia coli strain Ecol_545]
MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFATTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQP
KAELLTRTLQSALEQRASDIHIEPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLVLVTGPTGSGKTVTLYSALQTLN
TADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGVLRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNS
TCETLVRLQQMGVARWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPRWQAPGCRHCYHGFYGRTALFE
VLPITPIIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEELIRVLGMPHGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=177367 BE949_RS16410 WP_001550607.1 2865740..2867125(+) (pilB) [Escherichia coli strain Ecol_545]
ATGAATATTCCACAGCTCACCGCCCTGTGTCTGCGTTATCAGGGAGTCTTGCTGGATGCCAGCGAAGAGGTGGTTCATGT
TGCGGTGGTCGATGCCCCTTCGCATGAGCTACTGGACGCATTGCATTTCGCTACCACCAAACGTATTGAGATCACCTGCT
GGACGCGCCAACAAATGGAAGGTCACGCCAGTCGCACACAACAGACATTGCCCGTAGCTGTTCAGGAGAAGCATCAGCCC
AAAGCAGAGTTGCTGACTCGAACGTTACAATCTGCGCTGGAACAACGCGCGTCTGATATTCATATCGAACCAGCGGACAA
TGCCTACCGCATCCGCTTGCGTATCGACGGCGTATTGCATCCTTTACCGGATGTTTCACCGGATGCCGGAGTCGCATTAA
CCGCCAGATTAAAAGTGCTGGGCAACCTGGATATTGCGGAACATCGCCTGCCGCAGGACGGGCAATTCACTGTCGAACTG
GCAGGAAACGCCGTCTCATTTCGTATTGCGACCTTACCATGTCGGGGTGGTGAAAAGGTGGTATTAAGATTGTTACAGCA
GGTGAGTCAGGCACTGGATGTCAACACGCTTGGAATGCAGCCGTTACAACTGGCGGACTTTGCTCATGCCTTGCAACAAC
CACAGGGACTGGTGCTGGTAACAGGCCCTACAGGCAGCGGCAAAACGGTCACGCTTTATAGTGCCCTGCAAACGCTGAAT
ACTGCTGATATTAATATTTGTAGCGTCGAAGATCCGGTTGAGATCCCCATAGCCGGACTAAACCAGACGCAAATCCATCC
GCGTGCCGGTCTTACCTTTCAGGGCGTTTTGCGTGCGTTATTGCGCCAGGACCCTGACGTCATTATGATCGGAGAGATCC
GCGATGGCGAAACAGCAGAGATCGCTATTAAAGCGGCGCAAACCGGTCACCTGGTGTTGTCTACCCTACACACCAACTCC
ACCTGCGAAACGCTGGTGCGTTTACAGCAAATGGGCGTTGCTCGCTGGATGCTCTCATCAGCGCTTACGCTGGTAATAGC
CCAGCGTCTGGTACGCAAACTTTGCCCACATTGTCGCCGACAGCAAGGGGAGCCAATCCATATTCCAGACAATGTATGGC
CGTCGCCGCTGCCCCGCTGGCAAGCCCCTGGTTGTAGACACTGCTACCACGGTTTTTATGGTCGTACGGCCTTATTTGAA
GTTCTACCCATAACACCGATCATACGCCAGCTTATTTCCGCTAATACCGACGTTGAATCGCTGGAAACACACGCCCGACA
GGCGGGTATGCGTACGCTTTTTGAAAACGGCTGCCTGGCCGTGGAGCAAGGCTTAACCACCTTTGAAGAGTTAATCCGCG
TATTGGGGATGCCGCATGGCGAGTAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(8-68)

Type II/IV secretion system protein (T2SSE)

(88-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

50

83.297

0.416
  pilB Glaesserella parasuis strain SC1401

41.253

100

0.414
  pilB Acinetobacter baylyi ADP1

40.385

100

0.41
  pilB Vibrio campbellii strain DS40M4

48.446

83.731

0.406
  pilB Vibrio cholerae strain A1552

46.667

84.599

0.395
  pilB Vibrio parahaemolyticus RIMD 2210633

46.667

84.599

0.395
  pilB Haemophilus influenzae 86-028NP

45.22

83.948

0.38
  pilB Haemophilus influenzae Rd KW20

44.444

83.948

0.373
  pilF Neisseria gonorrhoeae MS11

44.416

83.514

0.371
  pilB Acinetobacter baumannii D1279779

43.846

84.599

0.371
  pilF Thermus thermophilus HB27

41.278

88.286

0.364