Detailed information NTG0176725

Bioinformatically predicted

Overview

• Name: comM
• Type: Machinery gene
• Locus tag: AMD27_RS14640
• Genome accession: NZ_CP015110
• Coordinates: 3061325..3062383 (-)
• Length: 352 a.a.
• NCBI ID: WP_067661866.1
• Uniprot ID: A0A143G773
• Organism: Acinetobacter sp. TGL-Y2
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3056325..3067383

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AMD27_RS14615 (AMD27_14615)	aroK	3056671..3057213 (-)	543	WP_067661857.1	shikimate kinase AroK	-
AMD27_RS14620 (AMD27_14620)	pilQ	3057250..3059427 (-)	2178	WP_067661859.1	type IV pilus secretin PilQ	Machinery gene
AMD27_RS14625 (AMD27_14625)	comL	3059447..3059941 (-)	495	WP_067661861.1	pilus assembly protein PilP	Machinery gene
AMD27_RS14630 (AMD27_14630)	comO	3059974..3060705 (-)	732	WP_067661862.1	type 4a pilus biogenesis protein PilO	Machinery gene
AMD27_RS14635 (AMD27_14635)	pilN	3060702..3061325 (-)	624	WP_067661864.1	PilN domain-containing protein	Machinery gene
AMD27_RS14640 (AMD27_14640)	comM	3061325..3062383 (-)	1059	WP_067661866.1	pilus assembly protein PilM	Machinery gene
AMD27_RS14645 (AMD27_14645)	ponA	3062543..3065098 (+)	2556	WP_067661867.1	penicillin-binding protein PBP1a	-
AMD27_RS14650 (AMD27_14650)	-	3065170..3065976 (-)	807	WP_067661870.1	putative RNA methyltransferase	-
AMD27_RS14655 (AMD27_14655)	mraY	3066045..3067163 (-)	1119	WP_067661872.1	phospho-N-acetylmuramoyl-pentapeptide- transferase	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 39008.55 Da
• Isoelectric Point: 4.4782

>NTDB_id=176725 AMD27_RS14640 WP_067661866.1 3061325..3062383(-) (comM) [Acinetobacter sp. TGL-Y2]
MLGLYRKPKKGLIGVDISSTSVKLLELSVKNGRYWVESYGLSPLVDGSVVEKNILNVDNVADALERAMNIANPQSSNVAI
AVPTSMVIHKTIEMDAEMSDEEREVQIRMDAEQYIPFPLDEVSLDFEVLPEKLANPNRVNVLLVATRTENVDSRIEVLDL
VGLHPQVADVDSYALERSFDVFSDTLPIGVNLVGVLDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTQDVQTRYGLSYDE
AGRAKKDRTLPDDYETEVLLPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLSKLMQQKLGYRVTIANPFL
QMGFSPQIDLRKIENDAPSLLVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=176725 AMD27_RS14640 WP_067661866.1 3061325..3062383(-) (comM) [Acinetobacter sp. TGL-Y2]
GTGCTTGGACTGTATCGTAAGCCAAAAAAGGGGTTAATAGGAGTAGACATTAGTTCTACTTCTGTAAAGTTATTGGAACT
CTCTGTAAAAAATGGACGCTATTGGGTTGAAAGCTACGGCTTAAGCCCGCTGGTGGACGGAAGTGTCGTTGAAAAAAATA
TTTTAAATGTGGACAATGTTGCTGATGCTTTAGAGCGTGCAATGAACATTGCCAATCCACAATCGTCCAATGTAGCGATT
GCAGTGCCAACCTCGATGGTGATTCATAAAACGATTGAGATGGACGCTGAGATGTCTGATGAAGAACGTGAAGTTCAAAT
TCGTATGGATGCTGAACAATATATCCCTTTCCCGCTCGATGAAGTTAGCCTTGATTTTGAAGTGCTCCCTGAAAAATTAG
CCAATCCAAACCGTGTCAATGTTTTATTGGTGGCGACCCGAACCGAAAACGTTGACTCACGTATTGAAGTTCTAGATTTG
GTCGGATTACATCCCCAAGTAGCAGATGTAGACAGTTATGCTTTAGAACGTAGTTTCGACGTGTTTTCAGACACTTTGCC
AATTGGGGTAAATCTGGTGGGTGTGTTAGATATTGGTCATACCATGACCACTTTATCGGTTATGCAAAACGGTAAAATTA
TCTATACCCGTGAACAGGTCTTTGGCGGTAAGCAGCTGACACAAGATGTTCAAACTCGTTATGGCTTGTCATATGATGAA
GCGGGGCGTGCTAAAAAAGATCGCACACTGCCAGATGATTATGAAACCGAAGTGTTATTACCTTTTTTAGATGCCGTGGT
TCAACAAGCCGCACGTTCATTGCAATTCTTTTTCTCTTCATCTCAGTTCAATGAAATTGATCATATTTTGCTGGCAGGTG
GTAATGCCAATATCCCAGGTCTATCAAAGCTCATGCAGCAAAAGCTTGGGTACCGCGTAACCATAGCCAACCCATTTTTA
CAAATGGGTTTTTCTCCTCAAATTGATCTTCGAAAAATAGAAAATGACGCACCCTCTTTATTGGTTGCCTGTGGATTAGC
ATTGAGGAGCTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: A0A143G773

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comM	Acinetobacter nosocomialis M2	82.955	100	0.83
pilM	Acinetobacter baumannii D1279779	82.67	100	0.827
comM	Acinetobacter baylyi ADP1	79.261	100	0.793
pilM	Legionella pneumophila strain ERS1305867	41.69	100	0.42